Restriction map for
pF1KB5606
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BanI BbeI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI Psp1406I PstI PvuI PvuII
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 1601 CGCG CG^CG
ApaI 739 GGGCCC GGGCC^C
AseI 1408 ATTAAT AT^TAAT
AsnI 1408 ATTAAT AT^TAAT
AvaI 1638 CCCGGG C^[CT]CG[GA]G
BalI 1116 TGGCCA TGG^CCA
BamHI 1532 GGATCC G^GATCC
BbrPI 1312 CACGTG CAC^GTG
BbvCI 607 CCTCAGC CCTCAGC(-5/-2)
BciVI 1035 GTATCC GTATCC(6/5)
BfaI 223 CTAG C^TAG
BsaAI 1312 CACGTG [CT]AC^GT[GA]
BsoBI 1638 CCCGGG C^[CT]CG[GA]G
BsrBI 1453 GAGCGG CCGCTC(-3/-3)
BstEII 961 GGTCACC G^GT[ACGT]ACC
BstPI 961 GGTCACC G^GT[ACGT]ACC
BstUI 1601 CGCG CG^CG
EarI 286 GAAGAG CTCTTC(1/4)
EcoO65I 961 GGTCACC G^GT[ACGT]ACC
HaeII 338 GGCGCT [GA]GCGC^[CT]
Ksp632I 286 GAAGAG CTCTTC(1/4)
KspI 1600 CCGCGG CCGC^GG
MaeI 223 CTAG C^TAG
MluNI 1116 TGGCCA TGG^CCA
MscI 1116 TGGCCA TGG^CCA
MvnI 1601 CGCG CG^CG
NcoI 1295 CCATGG C^CATGG
PmaCI 1312 CACGTG CAC^GTG
PmlI 1312 CACGTG CAC^GTG
PshAI 465 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1408 ATTAAT AT^TAAT
SacI 429 GAGCTC GAGCT^C
SacII 1600 CCGCGG CCGC^GG
SmaI 1638 CCCGGG CCC^GGG
XbaI 222 TCTAGA T^CTAGA
XmaCI 1638 CCCGGG C^CCGGG
XmaI 1638 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 108 GTCTAC GT^[AC][GT]AC
531 GTCTAC
AcsI 725 AAATTT [GA]^AATT[CT]
1591 AAATTT
ApoI 725 AAATTT [GA]^AATT[CT]
1591 AAATTT
AvaII 565 GGACC G^G[AT]CC
1048 GGTCC
BbsI 1056 GAAGAC GAAGAC(2/6)
1306 GTCTTC
BpuAI 1056 GAAGAC GAAGAC(2/6)
1306 GTCTTC
BsaI 70 GAGACC GGTCTC(1/5)
1720 GGTCTC
BsrDI 1100 CATTGC GCAATG(2/0)
1427 GCAATG
BstAPI 1331 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1778 GCAAGACCTGC
BstYI 1071 AGATCC [GA]^GATC[CT]
1532 GGATCC
CfoI 339 GCGC GCG^C
1093 GCGC
EcoNI 712 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1003 CCTGGAGCAGG
HgaI 352 GACGC GACGC(5/10)
881 GACGC
HhaI 339 GCGC GCG^C
1093 GCGC
HinP1I 339 GCGC G^CGC
1093 GCGC
HincII 953 GTCAAC GT[CT]^[GA]AC
1809 GTTGAC
HindII 953 GTCAAC GT[CT]^[GA]AC
1809 GTTGAC
MaeII 1265 ACGT A^CGT
1313 ACGT
MflI 1071 AGATCC [GA]^GATC[CT]
1532 GGATCC
MseI 662 TTAA T^TAA
1409 TTAA
MspA1I 1242 CCGCTG C[AC]G^C[GT]G
1600 CCGCGG
PleI 364 GACTC GAGTC(4/5)
934 GACTC
StuI 652 AGGCCT AGG^CCT
1478 AGGCCT
TaqI 463 TCGA T^CGA
1677 TCGA
Tru9I 662 TTAA T^TAA
1409 TTAA
Tsp509I 726 AATT ^AATT
1592 AATT
TthHB8I 463 TCGA T^CGA
1677 TCGA
XcmI 1017 CCAGAACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1439 CCAAGCCCATCATGG
XhoII 1071 AGATCC [GA]^GATC[CT]
1532 GGATCC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TfiI TseI Tsp45I TspRI Van91I