Restriction map for
pF1KB9259
##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BbrPI BcgI
BclI BfrI BlnI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MseI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1065 TCCGGA T^CCGGA
AgeI 1138 ACCGGT A^CCGGT
AhdI 971 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 971 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 598 GCTGAGG CCTCAGC(-5/-2)
BciVI 1027 GGATAC GTATCC(6/5)
BfaI 500 CTAG C^TAG
BglII 2906 AGATCT A^GATCT
BlpI 1386 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1386 GCTCAGC GC^T[ACGT]AGC
BsaAI 2336 CACGTA [CT]AC^GT[GA]
BsaBI 2907 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1069 GAGACC GGTCTC(1/5)
BseAI 1065 TCCGGA T^CCGGA
BsiWI 2237 CGTACG C^GTACG
BsmBI 1918 GAGACG CGTCTC(1/5)
BspEI 1065 TCCGGA T^CCGGA
BssSI 1696 CACGAG CACGAG(-5/-1)
CelII 1386 GCTCAGC GC^T[ACGT]AGC
Eam1105I 971 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 2301 GGCCGGCC GGCCGG^CC
MaeI 500 CTAG C^TAG
MamI 2907 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1065 TCCGGA T^CCGGA
NdeI 64 CATATG CA^TATG
PinAI 1138 ACCGGT A^CCGGT
SacI 1344 GAGCTC GAGCT^C
SexAI 515 ACCTGGT A^CC[AT]GGT
SphI 1490 GCATGC GCATG^C
TfiI 1930 GATTC G^A[AT]TC
XcmI 1014 CCAGGGCCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 276 GTAC GT^AC
2238 GTAC
AlwNI 1405 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2608 CAGGACCTG
AocI 217 CCTGAGG CC^T[ACGT]AGG
2716 CCTAAGG
Aor51HI 2015 AGCGCT AGC^GCT
2789 AGCGCT
AspHI 1344 GAGCTC G[AT]GC[AT]^C
2428 GAGCAC
BamHI 1331 GGATCC G^GATCC
2577 GGATCC
BbeI 564 GGCGCC GGCGC^C
1791 GGCGCC
BsaWI 1065 TCCGGA [AT]^CCGG[AT]
1138 ACCGGT
BsiHKAI 1344 GAGCTC G[AT]GC[AT]^C
2428 GAGCAC
BsrBI 1179 GAGCGG CCGCTC(-3/-3)
1215 GAGCGG
BstEII 7 GGTTACC G^GT[ACGT]ACC
1129 GGTTACC
BstPI 7 GGTTACC G^GT[ACGT]ACC
1129 GGTTACC
BstXI 956 CCATGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1965 CCACTCTATTGG
Bsu36I 217 CCTGAGG CC^T[ACGT]AGG
2716 CCTAAGG
EarI 605 GAAGAG CTCTTC(1/4)
1987 GAAGAG
Eco47III 2015 AGCGCT AGC^GCT
2789 AGCGCT
Eco81I 217 CCTGAGG CC^T[ACGT]AGG
2716 CCTAAGG
EcoO65I 7 GGTTACC G^GT[ACGT]ACC
1129 GGTTACC
KasI 564 GGCGCC G^GCGCC
1791 GGCGCC
Ksp632I 605 GAAGAG CTCTTC(1/4)
1987 GAAGAG
MaeII 71 ACGT A^CGT
2337 ACGT
NarI 564 GGCGCC GG^CGCC
1791 GGCGCC
NspI 950 GCATGT [GA]CATG^[CT]
1490 GCATGC
PvuII 1408 CAGCTG CAG^CTG
2697 CAGCTG
RsaI 276 GTAC GT^AC
2238 GTAC
SfoI 564 GGCGCC GGC^GCC
1791 GGCGCC
SgrAI 1137 CACCGGTG C[GA]^CCGG[CT]G
1793 CGCCGGTG
StuI 116 AGGCCT AGG^CCT
2073 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
ApaI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI