Restriction map for
pF1KE1019
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsgI BsiEI BsiWI
Bsp1407I BspEI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstZ17I CelII CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoNI
EcoRI EcoRV FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspI
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AatII 508 GACGTC GACGT^C
Acc65I 814 GGTACC G^GTACC
AccII 422 CGCG CG^CG
AlwNI 1234 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 981 AGCGCT AGC^GCT
Asp700I 1209 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 814 GGTACC G^GTACC
AspHI 735 GAGCTC G[AT]GC[AT]^C
AvaI 108 CTCGAG C^[CT]CG[GA]G
BalI 123 TGGCCA TGG^CCA
BamHI 1094 GGATCC G^GATCC
BanI 814 GGTACC G^G[CT][GA]CC
BanII 735 GAGCTC G[GA]GC[CT]^C
BbsI 226 GAAGAC GAAGAC(2/6)
BglI 1264 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 735 GAGCTC G[AGT]GC[ACT]^C
BpuAI 226 GAAGAC GAAGAC(2/6)
BsaBI 1936 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 462 GGTCTC GGTCTC(1/5)
BseRI 661 GAGGAG GAGGAG(10/8)
BsiHKAI 735 GAGCTC G[AT]GC[AT]^C
BsmI 1016 GAATGC GAATGC(1/-1)
BsoBI 108 CTCGAG C^[CT]CG[GA]G
Bsp1286I 735 GAGCTC G[AGT]GC[ACT]^C
BspDI 185 ATCGAT AT^CGAT
BspHI 1424 TCATGA T^CATGA
BsrDI 1435 GCAATG GCAATG(2/0)
BsrFI 349 GCCGGT [GA]^CCGG[CT]
BstBI 1758 TTCGAA TT^CGAA
BstEII 829 GGTGACC G^GT[ACGT]ACC
BstPI 829 GGTGACC G^GT[ACGT]ACC
BstUI 422 CGCG CG^CG
BstXI 1830 CCAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 982 GCGC GCG^C
Cfr10I 349 GCCGGT [GA]^CCGG[CT]
ClaI 185 ATCGAT AT^CGAT
DraII 1364 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 61 GACCAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1769 CCATGG C^C[GA][CT]GG
EaeI 123 TGGCCA [CT]^GGCC[GA]
Eco47III 981 AGCGCT AGC^GCT
EcoO109I 1364 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 829 GGTGACC G^GT[ACGT]ACC
EcoT22I 1469 ATGCAT ATGCA^T
HaeII 981 AGCGCT [GA]GCGC^[CT]
HapII 350 CCGG C^CGG
HhaI 982 GCGC GCG^C
HinP1I 982 GCGC G^CGC
HpaII 350 CCGG C^CGG
KpnI 814 GGTACC GGTAC^C
MamI 1936 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 123 TGGCCA TGG^CCA
MscI 123 TGGCCA TGG^CCA
MslI 1923 CACTTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 350 CCGG C^CGG
MvnI 422 CGCG CG^CG
NcoI 1769 CCATGG C^CATGG
NsiI 1469 ATGCAT ATGCA^T
NspV 1758 TTCGAA TT^CGAA
PaeR7I 108 CTCGAG C^TCGAG
PflMI 21 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1514 CAGCTG CAG^CTG
RcaI 1424 TCATGA T^CATGA
SacI 735 GAGCTC GAGCT^C
SfcI 1306 CTACAG C^T[GA][CT]AG
SfuI 1758 TTCGAA TT^CGAA
SspI 1894 AATATT AAT^ATT
StuI 1083 AGGCCT AGG^CCT
Van91I 21 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 108 CTCGAG C^TCGAG
XmnI 1209 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1375 GAATTT [GA]^AATT[CT]
1501 GAATTT
AcyI 508 GACGTC G[GA]^CG[CT]C
666 GACGCC
AocI 658 CCTGAGG CC^T[ACGT]AGG
1723 CCTCAGG
ApoI 1375 GAATTT [GA]^AATT[CT]
1501 GAATTT
AvaII 60 GGACC G^G[AT]CC
425 GGACC
BsaHI 508 GACGTC G[GA]^CG[CT]C
666 GACGCC
BsmBI 457 GAGACG CGTCTC(1/5)
664 GAGACG
BstF5I 1329 CATCC GGATG(2/0)
210 GGATG
BstYI 384 AGATCC [GA]^GATC[CT]
1094 GGATCC
Bsu36I 658 CCTGAGG CC^T[ACGT]AGG
1723 CCTCAGG
Eco81I 658 CCTGAGG CC^T[ACGT]AGG
1723 CCTCAGG
FokI 1329 CATCC GGATG(9/13)
210 GGATG
HgaI 420 GACGC GACGC(5/10)
666 GACGC
Hin1I 508 GACGTC G[GA]^CG[CT]C
666 GACGCC
HphI 637 GGTGA GGTGA(8/7)
829 GGTGA
MaeII 509 ACGT A^CGT
1718 ACGT
MaeIII 576 GTCAC ^GT[ACGT]AC
830 GTGAC
MflI 384 AGATCC [GA]^GATC[CT]
1094 GGATCC
MspA1I 51 CAGCGG C[AC]G^C[GT]G
1514 CAGCTG
SapI 756 GAAGAGC GCTCTTC(1/4)
1212 GCTCTTC
Tsp45I 576 GTCAC ^GT[GC]AC
830 GTGAC
XcmI 155 CCATCAAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1954 CCAAATGCTGAGTGG
XhoII 384 AGATCC [GA]^GATC[CT]
1094 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI HaeIII HinfI
ItaI Ksp632I MaeI MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I