Restriction map for
pF1KE1024
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BbrPI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiWI Bsp1407I BspDI BspHI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI Eam1105I
EarI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI Ksp632I MamI MfeI MluI
MluNI MscI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 708 TCCGGA T^CCGGA
AviII 902 TGCGCA TGC^GCA
BamHI 1079 GGATCC G^GATCC
BfaI 955 CTAG C^TAG
BseAI 708 TCCGGA T^CCGGA
BsiEI 197 CGGCCG CG[GA][CT]^CG
BsmBI 4 GAGACG CGTCTC(1/5)
BsmI 1839 GAATGC GAATGC(1/-1)
BspEI 708 TCCGGA T^CCGGA
BstEII 1797 GGTGACC G^GT[ACGT]ACC
BstPI 1797 GGTGACC G^GT[ACGT]ACC
DraIII 554 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 834 GACCTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 197 CGGCCG C^GGCCG
EclXI 197 CGGCCG C^GGCCG
Eco52I 197 CGGCCG C^GGCCG
EcoO65I 1797 GGTGACC G^GT[ACGT]ACC
FspI 902 TGCGCA TGC^GCA
HindIII 2332 AAGCTT A^AGCTT
MaeI 955 CTAG C^TAG
MroI 708 TCCGGA T^CCGGA
MseI 1646 TTAA T^TAA
NaeI 1937 GCCGGC GCC^GGC
NgoMIV 1937 GCCGGC G^CCGGC
PstI 1882 CTGCAG CTGCA^G
ScaI 941 AGTACT AGT^ACT
SfiI 132 GGCCACTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1936 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1769 GCATGC GCATG^C
Tru9I 1646 TTAA T^TAA
XcmI 743 CCACGTCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1010 ACATGT A^C[GA][CT]GT
1625 ACATGT
AlwI 1080 GATCC GGATC(4/5)
1079 GGATC
AocI 539 CCTCAGG CC^T[ACGT]AGG
638 CCTCAGG
BbeI 15 GGCGCC GGCGC^C
46 GGCGCC
BbsI 399 GAAGAC GAAGAC(2/6)
1777 GAAGAC
BcgI 201 CGAGACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1242 CGAGATGTTTGC
BglII 1409 AGATCT A^GATCT
1475 AGATCT
BpuAI 399 GAAGAC GAAGAC(2/6)
1777 GAAGAC
BsaWI 678 TCCGGT [AT]^CCGG[AT]
708 TCCGGA
BspLU11I 1010 ACATGT A^CATGT
1625 ACATGT
BssSI 1240 CACGAG CACGAG(-5/-1)
1927 CACGAG
BstAPI 553 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1764 GCAGTGCATGC
Bsu36I 539 CCTCAGG CC^T[ACGT]AGG
638 CCTCAGG
Eco81I 539 CCTCAGG CC^T[ACGT]AGG
638 CCTCAGG
EcoNI 646 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2068 CCTGCCCCAGG
KasI 15 GGCGCC G^GCGCC
46 GGCGCC
KspI 53 CCGCGG CCGC^GG
341 CCGCGG
MaeII 745 ACGT A^CGT
977 ACGT
NarI 15 GGCGCC GG^CGCC
46 GGCGCC
NcoI 866 CCATGG C^CATGG
1736 CCATGG
PaeR7I 776 CTCGAG C^TCGAG
1256 CTCGAG
PflMI 1100 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1683 CCACACGCTGG
SacII 53 CCGCGG CCGC^GG
341 CCGCGG
SfcI 1437 CTACAG C^T[GA][CT]AG
1882 CTGCAG
SfoI 15 GGCGCC GGC^GCC
46 GGCGCC
StuI 284 AGGCCT AGG^CCT
1298 AGGCCT
Van91I 1100 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1683 CCACACGCTGG
XhoI 776 CTCGAG C^TCGAG
1256 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SmaI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI