Restriction map for
pF1KE1026
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AclI AcsI AflII AhdI Aor51HI
ApoI AscI AseI AsnI AspEI
AvrII BamHI BciVI BclI BfaI
BfrI BlnI BsaBI BsiEI BsiWI
BsmI BspDI BspHI BsrDI BstBI
BstEII BstPI ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI MaeI
MamI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1861 GACGTC GACGT^C
Acc65I 592 GGTACC G^GTACC
AccIII 2132 TCCGGA T^CCGGA
AgeI 262 ACCGGT A^CCGGT
AocI 1789 CCTGAGG CC^T[ACGT]AGG
Asp700I 1905 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 592 GGTACC G^GTACC
AspI 342 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1085 GCAGCCAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 739 AGATCT A^GATCT
BlpI 2322 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2322 GCTGAGC GC^T[ACGT]AGC
BseAI 2132 TCCGGA T^CCGGA
BspEI 2132 TCCGGA T^CCGGA
BspLU11I 2654 ACATGT A^CATGT
BsrBI 1452 CCGCTC CCGCTC(-3/-3)
BssSI 2370 CTCGTG CACGAG(-5/-1)
Bst1107I 15 GTATAC GTA^TAC
BstYI 739 AGATCT [GA]^GATC[CT]
BstZ17I 15 GTATAC GTA^TAC
Bsu36I 1789 CCTGAGG CC^T[ACGT]AGG
CelII 2322 GCTGAGC GC^T[ACGT]AGC
DraIII 200 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1789 CCTGAGG CC^T[ACGT]AGG
KpnI 592 GGTACC GGTAC^C
KspI 2216 CCGCGG CCGC^GG
MflI 739 AGATCT [GA]^GATC[CT]
MroI 2132 TCCGGA T^CCGGA
PflFI 342 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 262 ACCGGT A^CCGGT
SacII 2216 CCGCGG CCGC^GG
SalI 1858 GTCGAC G^TCGAC
SgrAI 626 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1804 CCCGGG CCC^GGG
StuI 2366 AGGCCT AGG^CCT
Tsp509I 1724 AATT ^AATT
Tth111I 342 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 739 AGATCT [GA]^GATC[CT]
XmaCI 1804 CCCGGG C^CCGGG
XmaI 1804 CCCGGG C^CCGGG
XmnI 1905 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 15 GTATAC GT^[AC][GT]AC
1858 GTCGAC
Alw44I 266 GTGCAC G^TGCAC
604 GTGCAC
ApaLI 266 GTGCAC G^TGCAC
604 GTGCAC
AviII 1526 TGCGCA TGC^GCA
2597 TGCGCA
BbeI 1944 GGCGCC GGCGC^C
2224 GGCGCC
BbsI 1905 GAAGAC GAAGAC(2/6)
1459 GTCTTC
BpuAI 1905 GAAGAC GAAGAC(2/6)
1459 GTCTTC
Bsp1407I 965 TGTACA T^GTACA
1959 TGTACA
BsrGI 965 TGTACA T^GTACA
1959 TGTACA
EcoNI 1910 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2268 CCTGGCGCAGG
FspI 1526 TGCGCA TGC^GCA
2597 TGCGCA
HinfI 1356 GAGTC G^A[ACGT]TC
2842 GAGTC
KasI 1944 GGCGCC G^GCGCC
2224 GGCGCC
NaeI 2518 GCCGGC GCC^GGC
2877 GCCGGC
NarI 1944 GGCGCC GG^CGCC
2224 GGCGCC
NgoMIV 2518 GCCGGC G^CCGGC
2877 GCCGGC
NspI 1256 ACATGC [GA]CATG^[CT]
2654 ACATGT
PleI 1356 GAGTC GAGTC(4/5)
2842 GAGTC
PpuMI 1826 AGGTCCT [GA]G^G[AT]CC[CT]
2585 GGGACCT
PvuII 484 CAGCTG CAG^CTG
691 CAGCTG
SacI 229 GAGCTC GAGCT^C
1762 GAGCTC
SfoI 1944 GGCGCC GGC^GCC
2224 GGCGCC
SspBI 965 TGTACA T^GTACA
1959 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BcnI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PmaCI PmlI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI