Restriction map for
pF1KE1027
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BciVI BfrI BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MslI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AhdI 2010 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1425 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2010 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2291 TGGCCA TGG^CCA
BamHI 1205 GGATCC G^GATCC
BbrPI 2191 CACGTG CAC^GTG
BbsI 2313 GAAGAC GAAGAC(2/6)
BcgI 2978 GCACCAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1571 TGATCA T^GATCA
BglII 1251 AGATCT A^GATCT
BpuAI 2313 GAAGAC GAAGAC(2/6)
BsaAI 2191 CACGTG [CT]AC^GT[GA]
BsmBI 662 GAGACG CGTCTC(1/5)
BssHII 1748 GCGCGC G^CGCGC
BstAPI 2721 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3044 CCAGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 2010 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1571 TGATCA T^GATCA
MluNI 2291 TGGCCA TGG^CCA
MscI 2291 TGGCCA TGG^CCA
MseI 2000 TTAA T^TAA
NaeI 1501 GCCGGC GCC^GGC
NgoMIV 1501 GCCGGC G^CCGGC
PmaCI 2191 CACGTG CAC^GTG
PmlI 2191 CACGTG CAC^GTG
SacI 2074 GAGCTC GAGCT^C
SbfI 1314 CCTGCAGG CCTGCA^GG
Sse8387I 1314 CCTGCAGG CCTGCA^GG
Tru9I 2000 TTAA T^TAA
XbaI 2025 TCTAGA T^CTAGA
XmnI 1425 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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BbvCI 1949 CCTCAGC CCTCAGC(-5/-2)
297 GCTGAGG
BfaI 1030 CTAG C^TAG
2026 CTAG
BsmAI 10 GAGAC GTCTC(1/5)
662 GAGAC
BsmI 1831 GCATTC GAATGC(1/-1)
2681 GCATTC
BsrFI 1501 GCCGGC [GA]^CCGG[CT]
1901 GCCGGT
Cfr10I 1501 GCCGGC [GA]^CCGG[CT]
1901 GCCGGT
EagI 378 CGGCCG C^GGCCG
1184 CGGCCG
EclXI 378 CGGCCG C^GGCCG
1184 CGGCCG
Eco52I 378 CGGCCG C^GGCCG
1184 CGGCCG
HgaI 2316 GACGC GACGC(5/10)
2109 GCGTC
MaeI 1030 CTAG C^TAG
2026 CTAG
NcoI 1410 CCATGG C^CATGG
2765 CCATGG
SapI 172 GAAGAGC GCTCTTC(1/4)
2170 GAAGAGC
SfiI 1671 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2958 GGCCTTCCAGGCC
SmlI 943 CTCAAG C^T[CT][GA]AG
2782 CTCAAG
TfiI 1921 GATTC G^A[AT]TC
2794 GAATC
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI