Restriction map for
pF1KB6654
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tru9I
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 82 GTCGAC GT^[AC][GT]AC
AflIII 669 ACGTGT A^C[GA][CT]GT
AlwNI 43 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 361 CCCGAG C^[CT]CG[GA]G
BalI 128 TGGCCA TGG^CCA
BbsI 530 GTCTTC GAAGAC(2/6)
BcnI 193 CCCGG CC^[GC]GG
BlpI 72 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 72 GCTCAGC GC^T[ACGT]AGC
BpuAI 530 GTCTTC GAAGAC(2/6)
BsaWI 709 TCCGGT [AT]^CCGG[AT]
BsoBI 361 CCCGAG C^[CT]CG[GA]G
BsrFI 102 GCCGGC [GA]^CCGG[CT]
BstAPI 42 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 72 GCTCAGC GC^T[ACGT]AGC
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
DraII 115 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 128 TGGCCA [CT]^GGCC[GA]
EcoO109I 115 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1140 GAATTC G^AATTC
EcoRV 265 GATATC GAT^ATC
HincII 82 GTCGAC GT[CT]^[GA]AC
HindII 82 GTCGAC GT[CT]^[GA]AC
HinfI 349 GAATC G^A[ACGT]TC
HphI 664 GGTGA GGTGA(8/7)
MaeII 669 ACGT A^CGT
MluNI 128 TGGCCA TGG^CCA
MscI 128 TGGCCA TGG^CCA
NaeI 102 GCCGGC GCC^GGC
NciI 193 CCCGG CC^[GC]GG
NcoI 500 CCATGG C^CATGG
NgoMIV 102 GCCGGC G^CCGGC
PflMI 384 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 115 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 117 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 82 GTCGAC G^TCGAC
SexAI 836 ACCTGGT A^CC[AT]GGT
SmlI 736 CTCAAG C^T[CT][GA]AG
SspI 1054 AATATT AAT^ATT
StuI 917 AGGCCT AGG^CCT
TfiI 349 GAATC G^A[AT]TC
Van91I 384 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 571 GAATTT [GA]^AATT[CT]
1140 GAATTC
AfaI 258 GTAC GT^AC
841 GTAC
AlwI 556 GATCC GGATC(4/5)
149 GGATC
ApoI 571 GAATTT [GA]^AATT[CT]
1140 GAATTC
BanII 70 GGGCTC G[GA]GC[CT]^C
160 GAGCCC
BmyI 70 GGGCTC G[AGT]GC[ACT]^C
160 GAGCCC
Bsp1286I 70 GGGCTC G[AGT]GC[ACT]^C
160 GAGCCC
BsrBI 19 CCGCTC CCGCTC(-3/-3)
111 GAGCGG
BstYI 149 GGATCT [GA]^GATC[CT]
555 AGATCC
CfoI 25 GCGC GCG^C
633 GCGC
DpnII 150 GATC ^GATC
556 GATC
DsaI 164 CCACGG C^C[GA][CT]GG
500 CCATGG
EarI 577 CTCTTC CTCTTC(1/4)
1089 GAAGAG
EcoNI 324 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
909 CCTCACTGAGG
HgaI 122 GCGTC GACGC(5/10)
721 GCGTC
HhaI 25 GCGC GCG^C
633 GCGC
HinP1I 25 GCGC G^CGC
633 GCGC
Ksp632I 577 CTCTTC CTCTTC(1/4)
1089 GAAGAG
MboI 150 GATC ^GATC
556 GATC
MflI 149 GGATCT [GA]^GATC[CT]
555 AGATCC
MslI 936 CATTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
951 CACTCTAATG
NdeII 150 GATC ^GATC
556 GATC
PstI 49 CTGCAG CTGCA^G
686 CTGCAG
PvuII 46 CAGCTG CAG^CTG
185 CAGCTG
RsaI 258 GTAC GT^AC
841 GTAC
Sau3AI 150 GATC ^GATC
556 GATC
SfcI 49 CTGCAG C^T[GA][CT]AG
686 CTGCAG
XhoII 149 GGATCT [GA]^GATC[CT]
555 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BsaJI BseRI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp509I TspRI TthHB8I