Restriction map for
pF1KB9954
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BlnI BpmI
BsaAI BsaWI BseAI BseRI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRV
FauI FseI FspI KasI KpnI
KspI MluI MluNI MroI MscI
NarI NciI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 100 CGCG CG^CG
AcyI 1190 GACGCC G[GA]^CG[CT]C
Aor51HI 163 AGCGCT AGC^GCT
BamHI 1795 GGATCC G^GATCC
BciVI 1597 GTATCC GTATCC(6/5)
BglII 1623 AGATCT A^GATCT
BlpI 1270 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1270 GCTAAGC GC^T[ACGT]AGC
BsaBI 886 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1190 GACGCC G[GA]^CG[CT]C
BsaI 266 GAGACC GGTCTC(1/5)
BsgI 1427 GTGCAG GTGCAG(16/14)
BsmBI 661 CGTCTC CGTCTC(1/5)
BspMI 1830 ACCTGC ACCTGC(4/8)
BsrBI 572 GAGCGG CCGCTC(-3/-3)
BsrFI 1193 GCCGGC [GA]^CCGG[CT]
BstEII 565 GGTGACC G^GT[ACGT]ACC
BstPI 565 GGTGACC G^GT[ACGT]ACC
BstUI 100 CGCG CG^CG
CelII 1270 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1193 GCCGGC [GA]^CCGG[CT]
DraI 1999 TTTAAA TTT^AAA
DsaI 521 CCGTGG C^C[GA][CT]GG
Eco47III 163 AGCGCT AGC^GCT
EcoO65I 565 GGTGACC G^GT[ACGT]ACC
HaeII 163 AGCGCT [GA]GCGC^[CT]
HapII 1194 CCGG C^CGG
Hin1I 1190 GACGCC G[GA]^CG[CT]C
HindIII 41 AAGCTT A^AGCTT
HinfI 1644 GAGTC G^A[ACGT]TC
HpaI 1227 GTTAAC GTT^AAC
HpaII 1194 CCGG C^CGG
MamI 886 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 908 CAATTG C^AATTG
MspI 1194 CCGG C^CGG
MunI 908 CAATTG C^AATTG
MvnI 100 CGCG CG^CG
NaeI 1193 GCCGGC GCC^GGC
NgoMIV 1193 GCCGGC G^CCGGC
NspI 949 GCATGT [GA]CATG^[CT]
PleI 1644 GAGTC GAGTC(4/5)
SmlI 914 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 99 CCGC CCGC(-3/-1)
574 GCGG
AlwI 1796 GATCC GGATC(4/5)
1795 GGATC
AspHI 407 GAGCAC G[AT]GC[AT]^C
1820 GTGCTC
BglI 98 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
176 GCCAGTTGGGC
BmyI 407 GAGCAC G[AGT]GC[ACT]^C
1820 GTGCTC
BsiHKAI 407 GAGCAC G[AT]GC[AT]^C
1820 GTGCTC
Bsp1286I 407 GAGCAC G[AGT]GC[ACT]^C
1820 GTGCTC
BsrDI 185 GCAATG GCAATG(2/0)
1000 GCAATG
BstYI 1623 AGATCT [GA]^GATC[CT]
1795 GGATCC
CfoI 101 GCGC GCG^C
164 GCGC
EarI 704 GAAGAG CTCTTC(1/4)
944 GAAGAG
EcoRI 1350 GAATTC G^AATTC
1400 GAATTC
HgaI 1190 GACGC GACGC(5/10)
660 GCGTC
HhaI 101 GCGC GCG^C
164 GCGC
HinP1I 101 GCGC G^CGC
164 GCGC
HincII 1227 GTTAAC GT[CT]^[GA]AC
1318 GTCAAC
HindII 1227 GTTAAC GT[CT]^[GA]AC
1318 GTCAAC
Ksp632I 704 GAAGAG CTCTTC(1/4)
944 GAAGAG
MaeII 75 ACGT A^CGT
585 ACGT
MflI 1623 AGATCT [GA]^GATC[CT]
1795 GGATCC
MslI 205 CATGATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
232 CACCATCATG
MspA1I 271 CAGCTG C[AC]G^C[GT]G
695 CAGCTG
PstI 125 CTGCAG CTGCA^G
511 CTGCAG
PvuII 271 CAGCTG CAG^CTG
695 CAGCTG
SapI 704 GAAGAGC GCTCTTC(1/4)
944 GAAGAGC
Tsp45I 553 GTGAC ^GT[GC]AC
566 GTGAC
XhoII 1623 AGATCT [GA]^GATC[CT]
1795 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbsI BbvI BclI BfaI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BspHI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NsiI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp509I TspRI