Restriction map for
pF1KE1043
##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BciVI BclI BfaI BfrI
BglI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SgrAI SmaI SnaBI SpeI SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 493 GTCTAC GT^[AC][GT]AC
AccII 76 CGCG CG^CG
AcsI 1245 GAATTT [GA]^AATT[CT]
AcyI 410 GGCGCC G[GA]^CG[CT]C
AlwNI 868 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 163 CCTGAGG CC^T[ACGT]AGG
ApoI 1245 GAATTT [GA]^AATT[CT]
Asp700I 1242 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 692 CTCGGG C^[CT]CG[GA]G
BalI 833 TGGCCA TGG^CCA
BanII 1272 GGGCTC G[GA]GC[CT]^C
BbeI 410 GGCGCC GGCGC^C
BbrPI 1263 CACGTG CAC^GTG
BbvCI 1404 CCTCAGC CCTCAGC(-5/-2)
BcgI 876 GCAGGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 194 AGATCT A^GATCT
BlpI 360 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 360 GCTGAGC GC^T[ACGT]AGC
BsaAI 1263 CACGTG [CT]AC^GT[GA]
BsaHI 410 GGCGCC G[GA]^CG[CT]C
BsaI 1216 GAGACC GGTCTC(1/5)
BsmAI 1216 GAGAC GTCTC(1/5)
BsmI 1048 GCATTC GAATGC(1/-1)
BsoBI 692 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1094 TGTACA T^GTACA
BsrFI 857 GCCGGC [GA]^CCGG[CT]
BsrGI 1094 TGTACA T^GTACA
BstBI 1334 TTCGAA TT^CGAA
BstUI 76 CGCG CG^CG
BstXI 1258 CCAGGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 163 CCTGAGG CC^T[ACGT]AGG
CelII 360 GCTGAGC GC^T[ACGT]AGC
Cfr10I 857 GCCGGC [GA]^CCGG[CT]
DraII 698 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 163 CCTGAGG CC^T[ACGT]AGG
EcoO109I 698 AGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 410 GGCGCC [GA]GCGC^[CT]
Hin1I 410 GGCGCC G[GA]^CG[CT]C
KasI 410 GGCGCC G^GCGCC
MluNI 833 TGGCCA TGG^CCA
MscI 833 TGGCCA TGG^CCA
MvnI 76 CGCG CG^CG
NaeI 857 GCCGGC GCC^GGC
NarI 410 GGCGCC GG^CGCC
NgoMIV 857 GCCGGC G^CCGGC
NspV 1334 TTCGAA TT^CGAA
PmaCI 1263 CACGTG CAC^GTG
PmlI 1263 CACGTG CAC^GTG
PpuMI 698 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 723 CAGCTG CAG^CTG
SbfI 126 CCTGCAGG CCTGCA^GG
SfoI 410 GGCGCC GGC^GCC
SfuI 1334 TTCGAA TT^CGAA
SphI 546 GCATGC GCATG^C
Sse8387I 126 CCTGCAGG CCTGCA^GG
SspBI 1094 TGTACA T^GTACA
StuI 452 AGGCCT AGG^CCT
Tsp509I 1246 AATT ^AATT
XmnI 1242 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 1401 GATCC GGATC(4/5)
1173 GGATC
AspHI 742 GTGCTC G[AT]GC[AT]^C
925 GTGCTC
AvaII 168 GGACC G^G[AT]CC
699 GGACC
BbsI 151 GTCTTC GAAGAC(2/6)
1366 GTCTTC
BcnI 207 CCCGG CC^[GC]GG
1394 CCCGG
BpuAI 151 GTCTTC GAAGAC(2/6)
1366 GTCTTC
BsiHKAI 742 GTGCTC G[AT]GC[AT]^C
925 GTGCTC
BsrDI 793 GCAATG GCAATG(2/0)
1061 GCAATG
BstAPI 584 GCACCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
867 GCAGGAGCTGC
BstYI 194 AGATCT [GA]^GATC[CT]
1400 AGATCC
EaeI 102 CGGCCA [CT]^GGCC[GA]
833 TGGCCA
EcoT14I 643 CCAAGG C^C[AT][AT]GG
971 CCAAGG
HinfI 149 GAGTC G^A[ACGT]TC
760 GACTC
MflI 194 AGATCT [GA]^GATC[CT]
1400 AGATCC
MslI 1018 CATAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1098 CATTCGTGTG
NciI 207 CCCGG CC^[GC]GG
1394 CCCGG
NspI 546 GCATGC [GA]CATG^[CT]
804 GCATGT
PleI 760 GACTC GAGTC(4/5)
149 GAGTC
StyI 643 CCAAGG C^C[AT][AT]GG
971 CCAAGG
TaqI 11 TCGA T^CGA
1335 TCGA
TthHB8I 11 TCGA T^CGA
1335 TCGA
XhoII 194 AGATCT [GA]^GATC[CT]
1400 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TseI
Tsp45I TspRI