Restriction map for
pF1KE1047
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI MaeI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 412 GTCTAC GT^[AC][GT]AC
AcsI 664 GAATTT [GA]^AATT[CT]
AcyI 430 GACGCC G[GA]^CG[CT]C
AfaI 598 GTAC GT^AC
AflIII 834 ACGTGT A^C[GA][CT]GT
AlwI 9 GGATC GGATC(4/5)
ApoI 664 GAATTT [GA]^AATT[CT]
AspI 1026 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 482 TGCGCA TGC^GCA
BanII 750 GAGCCC G[GA]GC[CT]^C
BbrPI 547 CACGTG CAC^GTG
BglI 685 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 533 AGATCT A^GATCT
BlpI 732 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 732 GCTGAGC GC^T[ACGT]AGC
BsaAI 547 CACGTG [CT]AC^GT[GA]
BsaHI 430 GACGCC G[GA]^CG[CT]C
BspMI 965 ACCTGC ACCTGC(4/8)
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BsrFI 896 ACCGGC [GA]^CCGG[CT]
BstF5I 183 CATCC GGATG(2/0)
CelII 732 GCTGAGC GC^T[ACGT]AGC
Cfr10I 896 ACCGGC [GA]^CCGG[CT]
FokI 183 CATCC GGATG(9/13)
FspI 482 TGCGCA TGC^GCA
Hin1I 430 GACGCC G[GA]^CG[CT]C
MaeIII 775 GTGAC ^GT[ACGT]AC
MseI 47 TTAA T^TAA
NcoI 785 CCATGG C^CATGG
PflFI 1026 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 637 GACTC GAGTC(4/5)
PmaCI 547 CACGTG CAC^GTG
PmlI 547 CACGTG CAC^GTG
RsaI 598 GTAC GT^AC
SexAI 770 ACCAGGT A^CC[AT]GGT
TfiI 24 GAATC G^A[AT]TC
Tru9I 47 TTAA T^TAA
Tsp45I 775 GTGAC ^GT[GC]AC
Tth111I 1026 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AspHI 544 GAGCAC G[AT]GC[AT]^C
949 GTGCTC
AvaI 130 CCCGAG C^[CT]CG[GA]G
608 CTCGGG
BbsI 336 GAAGAC GAAGAC(2/6)
427 GAAGAC
BcnI 258 CCCGG CC^[GC]GG
935 CCCGG
BpuAI 336 GAAGAC GAAGAC(2/6)
427 GAAGAC
BsaBI 4 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
534 GATCTTCATC
BseRI 975 CTCCTC GAGGAG(10/8)
790 GAGGAG
BsiHKAI 544 GAGCAC G[AT]GC[AT]^C
949 GTGCTC
BsoBI 130 CCCGAG C^[CT]CG[GA]G
608 CTCGGG
BsrI 15 ACTGG ACTGG(1/-1)
1029 CCAGT
BstYI 9 GGATCT [GA]^GATC[CT]
533 AGATCT
DraII 590 AGGTCCC [GA]G^G[ACGT]CC[CT]
1024 GGGACCC
EaeI 314 TGGCCG [CT]^GGCC[GA]
551 TGGCCG
EarI 105 GAAGAG CTCTTC(1/4)
910 GAAGAG
EcoO109I 590 AGGTCCC [GA]G^G[ACGT]CC[CT]
1024 GGGACCC
EcoT14I 785 CCATGG C^C[AT][AT]GG
849 CCTTGG
HinfI 24 GAATC G^A[ACGT]TC
637 GACTC
HphI 774 GGTGA GGTGA(8/7)
879 GGTGA
Ksp632I 105 GAAGAG CTCTTC(1/4)
910 GAAGAG
MamI 4 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
534 GATCTTCATC
MflI 9 GGATCT [GA]^GATC[CT]
533 AGATCT
NciI 258 CCCGG CC^[GC]GG
935 CCCGG
PflMI 844 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
870 CCAGCTCCTGG
PpuMI 590 AGGTCCC [GA]G^G[AT]CC[CT]
1024 GGGACCC
PvuII 199 CAGCTG CAG^CTG
343 CAGCTG
StuI 393 AGGCCT AGG^CCT
677 AGGCCT
StyI 785 CCATGG C^C[AT][AT]GG
849 CCTTGG
TaqI 542 TCGA T^CGA
635 TCGA
TthHB8I 542 TCGA T^CGA
635 TCGA
Van91I 844 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
870 CCAGCTCCTGG
XhoII 9 GGATCT [GA]^GATC[CT]
533 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BbvI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
KspI MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PstI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp509I TspRI