Restriction map for
pF1KE1049
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstYI Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI DsaI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 280 GTATAC GT^[AC][GT]AC
Aor51HI 128 AGCGCT AGC^GCT
AspHI 67 GAGCAC G[AT]GC[AT]^C
BmyI 67 GAGCAC G[AGT]GC[ACT]^C
BseRI 11 GAGGAG GAGGAG(10/8)
BsiEI 38 CGGCCG CG[GA][CT]^CG
BsiHKAI 67 GAGCAC G[AT]GC[AT]^C
Bsp1286I 67 GAGCAC G[AGT]GC[ACT]^C
Bst1107I 280 GTATAC GTA^TAC
BstF5I 505 GGATG GGATG(2/0)
BstXI 170 CCATCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 280 GTATAC GTA^TAC
EaeI 38 CGGCCG [CT]^GGCC[GA]
EagI 38 CGGCCG C^GGCCG
EclXI 38 CGGCCG C^GGCCG
Eco47III 128 AGCGCT AGC^GCT
Eco52I 38 CGGCCG C^GGCCG
EcoNI 72 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 505 GGATG GGATG(9/13)
HindIII 591 AAGCTT A^AGCTT
HphI 87 GGTGA GGTGA(8/7)
MaeII 134 ACGT A^CGT
MaeIII 434 GTGAC ^GT[ACGT]AC
MslI 171 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 232 CAGCTG C[AC]G^C[GT]G
NotI 37 GCGGCCGC GC^GGCCGC
PstI 229 CTGCAG CTGCA^G
PvuII 232 CAGCTG CAG^CTG
SapI 144 GCTCTTC GCTCTTC(1/4)
SfaNI 119 GCATC GCATC(5/9)
SfcI 229 CTGCAG C^T[GA][CT]AG
TfiI 573 GATTC G^A[AT]TC
Tsp45I 434 GTGAC ^GT[GC]AC
TspRI 667 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 167 CCACCATCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 64 CGCG CG^CG
246 CGCG
AvaI 55 CCCGAG C^[CT]CG[GA]G
275 CCCGAG
BfaI 562 CTAG C^TAG
631 CTAG
BsaI 58 GAGACC GGTCTC(1/5)
640 GAGACC
BsmAI 58 GAGAC GTCTC(1/5)
640 GAGAC
BsoBI 55 CCCGAG C^[CT]CG[GA]G
275 CCCGAG
BsrI 203 ACTGG ACTGG(1/-1)
666 CCAGT
BstNI 30 CCTGG CC^[AT]GG
468 CCAGG
BstUI 64 CGCG CG^CG
246 CGCG
DraII 20 GGGACCC [GA]G^G[ACGT]CC[CT]
194 AGGTCCT
EarI 73 CTCTTC CTCTTC(1/4)
145 CTCTTC
EcoO109I 20 GGGACCC [GA]G^G[ACGT]CC[CT]
194 AGGTCCT
EcoRII 30 CCTGG ^CC[AT]GG
468 CCAGG
HaeII 51 AGCGCC [GA]GCGC^[CT]
128 AGCGCT
Ksp632I 73 CTCTTC CTCTTC(1/4)
145 CTCTTC
MaeI 562 CTAG C^TAG
631 CTAG
MseI 352 TTAA T^TAA
626 TTAA
MvaI 30 CCTGG CC^[AT]GG
468 CCAGG
MvnI 64 CGCG CG^CG
246 CGCG
NlaIII 270 CATG CATG^
493 CATG
PleI 216 GACTC GAGTC(4/5)
459 GAGTC
PpuMI 20 GGGACCC [GA]G^G[AT]CC[CT]
194 AGGTCCT
SmlI 190 CTCAAG C^T[CT][GA]AG
645 CTTGAG
Tru9I 352 TTAA T^TAA
626 TTAA
Tsp509I 479 AATT ^AATT
624 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BsiYI BslI BsmFI BssKI
Cac8I CfoI Cfr13I DpnII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MnlI MspI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI