Restriction map for
pF1KB6154
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BclI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FbaI FseI FspI
HaeII HgaI Hin1I HindIII KasI
KpnI KspI MaeIII MamI MluI
MluNI MroI MscI MslI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tsp45I Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 1290 GAATTC [GA]^AATT[CT]
AflII 1381 CTTAAG C^TTAAG
AflIII 1773 ACATGT A^C[GA][CT]GT
ApoI 1290 GAATTC [GA]^AATT[CT]
BamHI 193 GGATCC G^GATCC
BbsI 1914 GTCTTC GAAGAC(2/6)
BcnI 197 CCCGG CC^[GC]GG
BfrI 1381 CTTAAG C^TTAAG
BpuAI 1914 GTCTTC GAAGAC(2/6)
Bsp1407I 506 TGTACA T^GTACA
BspHI 1110 TCATGA T^CATGA
BspLU11I 1773 ACATGT A^CATGT
BspMI 1619 ACCTGC ACCTGC(4/8)
BsrDI 1471 GCAATG GCAATG(2/0)
BsrGI 506 TGTACA T^GTACA
BstXI 1151 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 31 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 6 GCGC GCG^C
DraI 463 TTTAAA TTT^AAA
DraIII 538 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 33 TGGCCG [CT]^GGCC[GA]
EcoRI 1290 GAATTC G^AATTC
FauI 1683 GCGGG CCCGC(4/6)
HapII 198 CCGG C^CGG
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
HpaI 1636 GTTAAC GTT^AAC
HpaII 198 CCGG C^CGG
HphI 604 TCACC GGTGA(8/7)
MaeII 1749 ACGT A^CGT
MfeI 1462 CAATTG C^AATTG
MspI 198 CCGG C^CGG
MunI 1462 CAATTG C^AATTG
MwoI 401 GCTTCCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 197 CCCGG CC^[GC]GG
PflMI 112 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 748 CTGCAG CTGCA^G
RcaI 1110 TCATGA T^CATGA
SspBI 506 TGTACA T^GTACA
SspI 1864 AATATT AAT^ATT
StuI 81 AGGCCT AGG^CCT
Van91I 112 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 876 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AseI 1138 ATTAAT AT^TAAT
1414 ATTAAT
AsnI 1138 ATTAAT AT^TAAT
1414 ATTAAT
AvaI 246 CCCGAG C^[CT]CG[GA]G
1327 CCCGAG
AvaII 243 GGACC G^G[AT]CC
1155 GGACC
BanI 715 GGTGCC G^G[CT][GA]CC
1336 GGTGCC
BcgI 1329 CGAGGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
279 GCAGTGTATTCG
BciVI 1449 GGATAC GTATCC(6/5)
477 GTATCC
BglII 380 AGATCT A^GATCT
1851 AGATCT
BsaI 249 GAGACC GGTCTC(1/5)
988 GGTCTC
BsoBI 246 CCCGAG C^[CT]CG[GA]G
1327 CCCGAG
BsrI 224 CCAGT ACTGG(1/-1)
1586 CCAGT
Cfr13I 243 GGACC G^G[ACGT]CC
1155 GGACC
DraII 242 AGGACCC [GA]G^G[ACGT]CC[CT]
1154 AGGACCT
EarI 445 GAAGAG CTCTTC(1/4)
1567 GAAGAG
EcoO109I 242 AGGACCC [GA]G^G[ACGT]CC[CT]
1154 AGGACCT
EcoT22I 658 ATGCAT ATGCA^T
1839 ATGCAT
HincII 206 GTCAAC GT[CT]^[GA]AC
1636 GTTAAC
HindII 206 GTCAAC GT[CT]^[GA]AC
1636 GTTAAC
Ksp632I 445 GAAGAG CTCTTC(1/4)
1567 GAAGAG
MspA1I 21 CAGCGG C[AC]G^C[GT]G
1681 CAGCGG
NsiI 658 ATGCAT ATGCA^T
1839 ATGCAT
NspI 1773 ACATGT [GA]CATG^[CT]
1841 GCATGT
PpuMI 242 AGGACCC [GA]G^G[AT]CC[CT]
1154 AGGACCT
PshBI 1138 ATTAAT AT^TAAT
1414 ATTAAT
Sau96I 243 GGACC G^G[ACGT]CC
1155 GGACC
ScaI 1079 AGTACT AGT^ACT
1630 AGTACT
SpeI 181 ACTAGT A^CTAGT
1695 ACTAGT
TaqI 288 TCGA T^CGA
1050 TCGA
TthHB8I 288 TCGA T^CGA
1050 TCGA
XcmI 503 CCATGTACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
975 CCAGAAATTAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
BanII BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmI
Bsp1286I BssKI BstF5I BstNI BstYI
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MboI MboII MflI MnlI MseI
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII XmnI