################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FokI FseI FspI HgaI HindIII HpaI KpnI KspI MaeIII MamI MfeI MluI MroI MseI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 193 GACGTC GACGT^C AfaI 186 GTAC GT^AC AspHI 633 GAGCTC G[AT]GC[AT]^C AvaI 18 CCCGGG C^[CT]CG[GA]G BalI 470 TGGCCA TGG^CCA BbeI 775 GGCGCC GGCGC^C BglI 443 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 890 AGATCT A^GATCT BsaI 626 GGTCTC GGTCTC(1/5) BsaWI 266 ACCGGA [AT]^CCGG[AT] BsiHKAI 633 GAGCTC G[AT]GC[AT]^C BsmBI 551 CGTCTC CGTCTC(1/5) BsoBI 18 CCCGGG C^[CT]CG[GA]G BstAPI 166 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EaeI 470 TGGCCA [CT]^GGCC[GA] EarI 606 CTCTTC CTCTTC(1/4) EcoRI 802 GAATTC G^AATTC HincII 37 GTCAAC GT[CT]^[GA]AC HindII 37 GTCAAC GT[CT]^[GA]AC KasI 775 GGCGCC G^GCGCC Ksp632I 606 CTCTTC CTCTTC(1/4) MluNI 470 TGGCCA TGG^CCA MscI 470 TGGCCA TGG^CCA NarI 775 GGCGCC GG^CGCC RsaI 186 GTAC GT^AC SacI 633 GAGCTC GAGCT^C SapI 605 GCTCTTC GCTCTTC(1/4) SfoI 775 GGCGCC GGC^GCC SmaI 18 CCCGGG CCC^GGG TfiI 418 GATTC G^A[AT]TC XcmI 845 CCATGGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 18 CCCGGG C^CCGGG XmaI 18 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 802 GAATTC [GA]^AATT[CT] 829 AAATTC AcyI 193 GACGTC G[GA]^CG[CT]C 775 GGCGCC AlwI 69 GGATC GGATC(4/5) 867 GGATC ApaI 22 GGGCCC GGGCC^C 690 GGGCCC ApoI 802 GAATTC [GA]^AATT[CT] 829 AAATTC BfaI 652 CTAG C^TAG 685 CTAG BsaHI 193 GACGTC G[GA]^CG[CT]C 775 GGCGCC BseRI 154 GAGGAG GAGGAG(10/8) 208 GAGGAG BsmAI 552 GTCTC GTCTC(1/5) 627 GTCTC BstXI 461 CCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 765 CCAGAGCTATGG BstYI 69 GGATCT [GA]^GATC[CT] 890 AGATCT HaeII 100 AGCGCC [GA]GCGC^[CT] 775 GGCGCC Hin1I 193 GACGTC G[GA]^CG[CT]C 775 GGCGCC HphI 137 TCACC GGTGA(8/7) 833 TCACC MaeI 652 CTAG C^TAG 685 CTAG MaeII 194 ACGT A^CGT 367 ACGT MboII 222 GAAGA GAAGA(8/7) 607 TCTTC MflI 69 GGATCT [GA]^GATC[CT] 890 AGATCT MslI 384 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 462 CACGGCAGTG NcoI 671 CCATGG C^CATGG 845 CCATGG PleI 238 GAGTC GAGTC(4/5) 502 GAGTC PpuMI 726 GGGACCT [GA]G^G[AT]CC[CT] 809 AGGACCC SfaNI 75 GATGC GCATC(5/9) 410 GCATC SfcI 423 CTACAG C^T[GA][CT]AG 780 CTACAG Tsp509I 803 AATT ^AATT 830 AATT XhoII 69 GGATCT [GA]^GATC[CT] 890 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AocI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI TspRI