Restriction map for
pF1KE4265
##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AccII AccIII AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspI
AviII BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KspI MamI MluI MroI MvnI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 2971 AACGTT AA^CGTT
AcyI 2507 GGCGTC G[GA]^CG[CT]C
AflIII 1962 ACATGT A^C[GA][CT]GT
AvrII 2580 CCTAGG C^CTAGG
BciVI 2901 GGATAC GTATCC(6/5)
BglII 583 AGATCT A^GATCT
BlnI 2580 CCTAGG C^CTAGG
BsaHI 2507 GGCGTC G[GA]^CG[CT]C
BsmBI 178 GAGACG CGTCTC(1/5)
BspLU11I 1962 ACATGT A^CATGT
BsrDI 2533 CATTGC GCAATG(2/0)
BsrFI 2696 GCCGGC [GA]^CCGG[CT]
BssSI 3012 CACGAG CACGAG(-5/-1)
BstAPI 2875 GCAAAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1170 GGTCACC G^GT[ACGT]ACC
BstPI 1170 GGTCACC G^GT[ACGT]ACC
BstXI 2118 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2696 GCCGGC [GA]^CCGG[CT]
DraI 418 TTTAAA TTT^AAA
EcoNI 3130 CCTACCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1170 GGTCACC G^GT[ACGT]ACC
HaeII 1767 GGCGCT [GA]GCGC^[CT]
HgaI 2508 GCGTC GACGC(5/10)
Hin1I 2507 GGCGTC G[GA]^CG[CT]C
HincII 2575 GTTGAC GT[CT]^[GA]AC
HindII 2575 GTTGAC GT[CT]^[GA]AC
MfeI 2801 CAATTG C^AATTG
MspA1I 3076 CAGCTG C[AC]G^C[GT]G
MunI 2801 CAATTG C^AATTG
NaeI 2696 GCCGGC GCC^GGC
NcoI 2673 CCATGG C^CATGG
NdeI 505 CATATG CA^TATG
NgoMIV 2696 GCCGGC G^CCGGC
Psp1406I 2971 AACGTT AA^CGTT
PvuII 3076 CAGCTG CAG^CTG
SapI 1283 GAAGAGC GCTCTTC(1/4)
SmaI 2609 CCCGGG CCC^GGG
SphI 2880 GCATGC GCATG^C
StuI 2665 AGGCCT AGG^CCT
XbaI 198 TCTAGA T^CTAGA
XcmI 286 CCAGTGAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2609 CCCGGG C^CCGGG
XmaI 2609 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1073 GGTACC G^GTACC
1826 GGTACC
AcsI 68 AAATTC [GA]^AATT[CT]
1593 AAATTC
AhdI 2439 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2578 GACCTAGGGTC
ApoI 68 AAATTC [GA]^AATT[CT]
1593 AAATTC
Asp718I 1073 GGTACC G^GTACC
1826 GGTACC
AspEI 2439 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2578 GACCTAGGGTC
AvaI 9 CCCGAG C^[CT]CG[GA]G
2609 CCCGGG
BsoBI 9 CCCGAG C^[CT]CG[GA]G
2609 CCCGGG
BspMI 3105 ACCTGC ACCTGC(4/8)
3141 ACCTGC
BsrBI 2539 CCGCTC CCGCTC(-3/-3)
2653 GAGCGG
BstYI 141 GGATCT [GA]^GATC[CT]
583 AGATCT
CfoI 1768 GCGC GCG^C
2661 GCGC
Eam1105I 2439 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2578 GACCTAGGGTC
EarI 1120 GAAGAG CTCTTC(1/4)
1283 GAAGAG
FauI 1091 CCCGC CCCGC(4/6)
2538 CCCGC
HhaI 1768 GCGC GCG^C
2661 GCGC
HinP1I 1768 GCGC G^CGC
2661 GCGC
KpnI 1073 GGTACC GGTAC^C
1826 GGTACC
Ksp632I 1120 GAAGAG CTCTTC(1/4)
1283 GAAGAG
MflI 141 GGATCT [GA]^GATC[CT]
583 AGATCT
PleI 1471 GACTC GAGTC(4/5)
2704 GACTC
PstI 1387 CTGCAG CTGCA^G
2620 CTGCAG
ScaI 767 AGTACT AGT^ACT
3037 AGTACT
SfcI 1387 CTGCAG C^T[GA][CT]AG
2620 CTGCAG
SmlI 348 CTTGAG C^T[CT][GA]AG
2710 CTCAAG
XhoII 141 GGATCT [GA]^GATC[CT]
583 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I