Restriction map for
pF1KE9630
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsoBI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MluI MroI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1821 GTCTAC GT^[AC][GT]AC
ApaI 3200 GGGCCC GGGCC^C
BalI 1199 TGGCCA TGG^CCA
BclI 632 TGATCA T^GATCA
BcnI 9 CCCGG CC^[GC]GG
BglII 2421 AGATCT A^GATCT
BlpI 2928 GCTCAGC GC^T[ACGT]AGC
BpmI 581 CTGGAG CTGGAG(16/14)
Bpu1102I 2928 GCTCAGC GC^T[ACGT]AGC
BsaBI 3118 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2000 CGTCTC CGTCTC(1/5)
BsmI 2699 GAATGC GAATGC(1/-1)
BspDI 2956 ATCGAT AT^CGAT
BstAPI 1810 GCACAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 273 CCATGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2928 GCTCAGC GC^T[ACGT]AGC
CfoI 1009 GCGC GCG^C
ClaI 2956 ATCGAT AT^CGAT
DraIII 1250 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1199 TGGCCA [CT]^GGCC[GA]
EcoNI 2041 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 588 GATATC GAT^ATC
EcoT22I 1493 ATGCAT ATGCA^T
FbaI 632 TGATCA T^GATCA
HaeII 1008 AGCGCC [GA]GCGC^[CT]
HapII 10 CCGG C^CGG
HhaI 1009 GCGC GCG^C
HinP1I 1009 GCGC G^CGC
HpaII 10 CCGG C^CGG
MamI 3118 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2341 CAATTG C^AATTG
MluNI 1199 TGGCCA TGG^CCA
MscI 1199 TGGCCA TGG^CCA
MspI 10 CCGG C^CGG
MunI 2341 CAATTG C^AATTG
NciI 9 CCCGG CC^[GC]GG
NsiI 1493 ATGCAT ATGCA^T
PshAI 2836 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2599 GAGCTC GAGCT^C
ScaI 1231 AGTACT AGT^ACT
SnaBI 3206 TACGTA TAC^GTA
StuI 1991 AGGCCT AGG^CCT
TaqI 2957 TCGA T^CGA
Tsp45I 239 GTGAC ^GT[GC]AC
TthHB8I 2957 TCGA T^CGA
XbaI 1476 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AciI 935 CCGC CCGC(-3/-1)
316 GCGG
AflIII 2006 ACATGT A^C[GA][CT]GT
2540 ACATGT
AlwI 2128 GATCC GGATC(4/5)
927 GGATC
AlwNI 997 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
2102 CAGACACTG
Asp700I 2734 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3088 GAATTTTTTC
BbsI 811 GAAGAC GAAGAC(2/6)
1016 GTCTTC
BpuAI 811 GAAGAC GAAGAC(2/6)
1016 GTCTTC
BsaAI 968 CACGTA [CT]AC^GT[GA]
3206 TACGTA
BseRI 1148 CTCCTC GAGGAG(10/8)
3029 GAGGAG
BsgI 1808 CTGCAC GTGCAG(16/14)
1366 GTGCAG
Bsp1407I 266 TGTACA T^GTACA
302 TGTACA
BspHI 1236 TCATGA T^CATGA
1412 TCATGA
BspLU11I 2006 ACATGT A^CATGT
2540 ACATGT
BspMI 696 GCAGGT ACCTGC(4/8)
2047 GCAGGT
BsrGI 266 TGTACA T^GTACA
302 TGTACA
FauI 934 CCCGC CCCGC(4/6)
316 GCGGG
HgaI 2110 GACGC GACGC(5/10)
1819 GCGTC
HindIII 721 AAGCTT A^AGCTT
1837 AAGCTT
MaeII 969 ACGT A^CGT
3207 ACGT
PpuMI 1651 AGGACCT [GA]G^G[AT]CC[CT]
2500 GGGACCC
RcaI 1236 TCATGA T^CATGA
1412 TCATGA
SmlI 461 CTCAAG C^T[CT][GA]AG
2669 CTCAAG
SphI 348 GCATGC GCATG^C
2990 GCATGC
SspBI 266 TGTACA T^GTACA
302 TGTACA
SspI 653 AATATT AAT^ATT
3109 AATATT
XcmI 1187 CCATGTTTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2367 CCATCACCTTCTTGG
XmnI 2734 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3088 GAATTTTTTC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AcsI AfaI AluI Alw44I ApaLI
ApoI AspHI AvaII BanI BanII
BbvI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI
Van91I XhoII