Restriction map for
pF1KB7945
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsgI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I EarI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 941 GGTACC G^GTACC
AccII 715 CGCG CG^CG
AcsI 200 AAATTC [GA]^AATT[CT]
AflIII 951 ACACGT A^C[GA][CT]GT
Aor51HI 1117 AGCGCT AGC^GCT
ApoI 200 AAATTC [GA]^AATT[CT]
Asp700I 601 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 941 GGTACC G^GTACC
BalI 842 TGGCCA TGG^CCA
BamHI 253 GGATCC G^GATCC
BanII 760 GAGCCC G[GA]GC[CT]^C
BfaI 389 CTAG C^TAG
BsiEI 521 CGGCCG CG[GA][CT]^CG
BsmBI 596 GAGACG CGTCTC(1/5)
BsrBI 361 GAGCGG CCGCTC(-3/-3)
BstUI 715 CGCG CG^CG
BstXI 278 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 253 GGATCC [GA]^GATC[CT]
DraII 148 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 492 GACGCAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 521 CGGCCG C^GGCCG
EclXI 521 CGGCCG C^GGCCG
Eco47III 1117 AGCGCT AGC^GCT
Eco52I 521 CGGCCG C^GGCCG
EcoO109I 148 AGGCCCT [GA]G^G[ACGT]CC[CT]
KpnI 941 GGTACC GGTAC^C
MaeI 389 CTAG C^TAG
MboII 746 TCTTC GAAGA(8/7)
MflI 253 GGATCC [GA]^GATC[CT]
MluNI 842 TGGCCA TGG^CCA
MscI 842 TGGCCA TGG^CCA
MvnI 715 CGCG CG^CG
NaeI 523 GCCGGC GCC^GGC
NgoMIV 523 GCCGGC G^CCGGC
PstI 1145 CTGCAG CTGCA^G
PvuII 697 CAGCTG CAG^CTG
SfiI 834 GGCCAATCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XhoII 253 GGATCC [GA]^GATC[CT]
XmnI 601 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 416 TCCGGA T^CCGGA
659 TCCGGA
AlwNI 147 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
694 CAGCAGCTG
AspHI 510 GAGCAC G[AT]GC[AT]^C
924 GAGCAC
BglI 802 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
835 GCCAATCTGGC
BseAI 416 TCCGGA T^CCGGA
659 TCCGGA
BsiHKAI 510 GAGCAC G[AT]GC[AT]^C
924 GAGCAC
BspEI 416 TCCGGA T^CCGGA
659 TCCGGA
BsrFI 347 ACCGGC [GA]^CCGG[CT]
523 GCCGGC
Cfr10I 347 ACCGGC [GA]^CCGG[CT]
523 GCCGGC
DsaI 183 CCATGG C^C[GA][CT]GG
797 CCATGG
EcoT14I 183 CCATGG C^C[AT][AT]GG
797 CCATGG
HaeII 571 AGCGCC [GA]GCGC^[CT]
1117 AGCGCT
HphI 507 GGTGA GGTGA(8/7)
749 TCACC
MaeII 562 ACGT A^CGT
953 ACGT
MroI 416 TCCGGA T^CCGGA
659 TCCGGA
MslI 136 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
184 CATGGTTGTG
NcoI 183 CCATGG C^CATGG
797 CCATGG
SfcI 1035 CTACAG C^T[GA][CT]AG
1145 CTGCAG
SmaI 937 CCCGGG CCC^GGG
1086 CCCGGG
StyI 183 CCATGG C^C[AT][AT]GG
797 CCATGG
TfiI 60 GAATC G^A[AT]TC
517 GATTC
XcmI 272 CCACACCCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
797 CCATGGCCTCGCTGG
XmaCI 937 CCCGGG C^CCGGG
1086 CCCGGG
XmaI 937 CCCGGG C^CCGGG
1086 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I Tsp509I TspRI