##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BclI        BcnI        BfaI        BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssKI       BssSI       Bst1107I    BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        ItaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TseI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AciI          663  GCGG                 CCGC(-3/-1)
AgeI          757  ACCGGT               A^CCGGT
Asp700I       971  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI         1244  GGCACC               G^G[CT][GA]CC
BciVI         146  GTATCC               GTATCC(6/5)
BglII         897  AGATCT               A^GATCT
BpmI         1010  CTGGAG               CTGGAG(16/14)
BsaWI         757  ACCGGT               [AT]^CCGG[AT]
BsrFI         757  ACCGGT               [GA]^CCGG[CT]
BstAPI        307  GCAAACCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        757  ACCGGT               [GA]^CCGG[CT]
EcoT22I      1003  ATGCAT               ATGCA^T
HapII         758  CCGG                 C^CGG
HpaII         758  CCGG                 C^CGG
MspI          758  CCGG                 C^CGG
MwoI          307  GCAAACCTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV        1244  GGCACC               GG[ACGT]^[ACGT]CC
NsiI         1003  ATGCAT               ATGCA^T
PinAI         757  ACCGGT               A^CCGGT
SmlI         1314  CTCAAG               C^T[CT][GA]AG
XmnI          971  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI         1166  AGCT                 AG^CT
             1233  AGCT                
BbsI          127  GAAGAC               GAAGAC(2/6)
             1044  GAAGAC              
BpuAI         127  GAAGAC               GAAGAC(2/6)
             1044  GAAGAC              
BsiYI         563  CCCTTATTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              653  CCCAACATCGG         
BslI          563  CCCTTATTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              653  CCCAACATCGG         
BstYI          85  GGATCT               [GA]^GATC[CT]
              897  AGATCT              
MboII         127  GAAGA                GAAGA(8/7)
             1044  GAAGA               
MflI           85  GGATCT               [GA]^GATC[CT]
              897  AGATCT              
MnlI           44  GAGG                 CCTC(7/6)
             1392  GAGG                
MseI          884  TTAA                 T^TAA
             1238  TTAA                
NlaIII        132  CATG                 CATG^
              701  CATG                
NspI          131  ACATGC               [GA]CATG^[CT]
              700  GCATGT              
Tru9I         884  TTAA                 T^TAA
             1238  TTAA                
XhoII          85  GGATCT               [GA]^GATC[CT]
              897  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AcsI        AlwI        ApoI        BsmI        BsrI        
DdeI        DpnII       EcoRI       HaeIII      HinfI       
HphI        MboI        MslI        NdeII       Sau3AI      
SfaNI       StuI        TaqI        TfiI        Tsp509I     
TspRI       TthHB8I