Restriction map for
pF1KB7312
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# NO CUT ENZYMES ( 79 enzymes) #
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Acc65I AclI AcsI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I BbsI BcgI BciVI BclI
BfrI BglII BpuAI BsaBI BsiWI
BsmBI BsmI BspHI BspLU11I Bst1107I
BstBI BstZ17I Bsu36I CpoI DraI
EarI Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI HindIII HpaI KpnI
Ksp632I KspI MamI MluI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 2611 GACGTC GACGT^C
AccIII 1885 TCCGGA T^CCGGA
AhdI 1166 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1166 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1444 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2014 TGCGCA TGC^GCA
AvrII 2646 CCTAGG C^CTAGG
BbrPI 166 CACGTG CAC^GTG
BglI 2413 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2646 CCTAGG C^CTAGG
BsaI 223 GAGACC GGTCTC(1/5)
BseAI 1885 TCCGGA T^CCGGA
BspDI 1413 ATCGAT AT^CGAT
BspEI 1885 TCCGGA T^CCGGA
BsrDI 1489 GCAATG GCAATG(2/0)
BssHII 2695 GCGCGC G^CGCGC
BssSI 28 CTCGTG CACGAG(-5/-1)
BstEII 1449 GGTCACC G^GT[ACGT]ACC
BstPI 1449 GGTCACC G^GT[ACGT]ACC
ClaI 1413 ATCGAT AT^CGAT
Eam1105I 1166 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1449 GGTCACC G^GT[ACGT]ACC
FseI 3103 GGCCGGCC GGCCGG^CC
FspI 2014 TGCGCA TGC^GCA
HincII 2038 GTCGAC GT[CT]^[GA]AC
HindII 2038 GTCGAC GT[CT]^[GA]AC
MfeI 389 CAATTG C^AATTG
MroI 1885 TCCGGA T^CCGGA
MseI 2144 TTAA T^TAA
MunI 389 CAATTG C^AATTG
PflFI 1444 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 166 CACGTG CAC^GTG
PmlI 166 CACGTG CAC^GTG
SalI 2038 GTCGAC G^TCGAC
SbfI 3041 CCTGCAGG CCTGCA^GG
SmlI 1000 CTCAAG C^T[CT][GA]AG
Sse8387I 3041 CCTGCAGG CCTGCA^GG
StuI 1483 AGGCCT AGG^CCT
Tru9I 2144 TTAA T^TAA
Tsp509I 390 AATT ^AATT
Tth111I 1444 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 2038 GTCGAC GT^[AC][GT]AC
2614 GTCTAC
AlwNI 295 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
1063 CAGGCACTG
BbeI 2457 GGCGCC GGCGC^C
2540 GGCGCC
BbvCI 786 CCTCAGC CCTCAGC(-5/-2)
2922 CCTCAGC
BlpI 300 GCTGAGC GC^T[ACGT]AGC
400 GCTGAGC
Bpu1102I 300 GCTGAGC GC^T[ACGT]AGC
400 GCTGAGC
BsaWI 197 TCCGGT [AT]^CCGG[AT]
1885 TCCGGA
Bsp1407I 848 TGTACA T^GTACA
2159 TGTACA
BsrGI 848 TGTACA T^GTACA
2159 TGTACA
CelII 300 GCTGAGC GC^T[ACGT]AGC
400 GCTGAGC
DraIII 25 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
1067 CACTGTGTG
DrdI 1009 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1774 GACCTGGCTGTC
KasI 2457 GGCGCC G^GCGCC
2540 GGCGCC
MboII 1713 GAAGA GAAGA(8/7)
956 TCTTC
NarI 2457 GGCGCC GG^CGCC
2540 GGCGCC
NcoI 1334 CCATGG C^CATGG
2885 CCATGG
NspI 1316 GCATGC [GA]CATG^[CT]
2972 GCATGC
PflMI 2443 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2627 CCAAGCAGTGG
SacI 403 GAGCTC GAGCT^C
1927 GAGCTC
SfoI 2457 GGCGCC GGC^GCC
2540 GGCGCC
SphI 1316 GCATGC GCATG^C
2972 GCATGC
SspBI 848 TGTACA T^GTACA
2159 TGTACA
TfiI 194 GAATC G^A[AT]TC
1226 GAATC
Van91I 2443 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2627 CCAAGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI