################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AspEI AspI AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI BstBI BstEII BstPI Bsu36I CelII ClaI CpoI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV FauI FbaI FseI HaeII HindIII HpaI KasI KspI MamI MfeI MluI MunI NarI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 1356 GACGTC GACGT^C Acc65I 2517 GGTACC G^GTACC AccII 2046 CGCG CG^CG AccIII 1914 TCCGGA T^CCGGA AlwNI 2357 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 2586 GGGCCC GGGCC^C AseI 454 ATTAAT AT^TAAT AsnI 454 ATTAAT AT^TAAT Asp718I 2517 GGTACC G^GTACC AspHI 856 GAGCAC G[AT]GC[AT]^C AviII 1886 TGCGCA TGC^GCA BcgI 1263 GCAACTATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 1190 TACGTG [CT]AC^GT[GA] BsaWI 1914 TCCGGA [AT]^CCGG[AT] BseAI 1914 TCCGGA T^CCGGA BsiEI 2544 CGGCCG CG[GA][CT]^CG BsiHKAI 856 GAGCAC G[AT]GC[AT]^C BsmBI 1378 CGTCTC CGTCTC(1/5) BspEI 1914 TCCGGA T^CCGGA BspHI 2250 TCATGA T^CATGA BsrDI 998 GCAATG GCAATG(2/0) BsrFI 2542 GCCGGC [GA]^CCGG[CT] Bst1107I 678 GTATAC GTA^TAC BstUI 2046 CGCG CG^CG BstZ17I 678 GTATAC GTA^TAC CfoI 1887 GCGC GCG^C Cfr10I 2542 GCCGGC [GA]^CCGG[CT] DraI 1783 TTTAAA TTT^AAA DraIII 2560 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2469 GACCATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2544 CGGCCG C^GGCCG EarI 318 GAAGAG CTCTTC(1/4) EclXI 2544 CGGCCG C^GGCCG Eco52I 2544 CGGCCG C^GGCCG EcoNI 2590 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1886 TGCGCA TGC^GCA HgaI 538 GACGC GACGC(5/10) HhaI 1887 GCGC GCG^C HinP1I 1887 GCGC G^CGC HincII 1626 GTCAAC GT[CT]^[GA]AC HindII 1626 GTCAAC GT[CT]^[GA]AC KpnI 2517 GGTACC GGTAC^C Ksp632I 318 GAAGAG CTCTTC(1/4) MroI 1914 TCCGGA T^CCGGA MvnI 2046 CGCG CG^CG NaeI 2542 GCCGGC GCC^GGC NdeI 1151 CATATG CA^TATG NgoMIV 2542 GCCGGC G^CCGGC NspI 1017 GCATGT [GA]CATG^[CT] PflMI 2115 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2644 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 454 ATTAAT AT^TAAT RcaI 2250 TCATGA T^CATGA SapI 318 GAAGAGC GCTCTTC(1/4) SexAI 2561 ACCTGGT A^CC[AT]GGT SspI 1171 AATATT AAT^ATT StuI 2579 AGGCCT AGG^CCT TaqI 1452 TCGA T^CGA TthHB8I 1452 TCGA T^CGA Van91I 2115 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 678 GTATAC GT^[AC][GT]AC 1536 GTCTAC AcyI 538 GACGCC G[GA]^CG[CT]C 1356 GACGTC AvaI 1818 CCCGAG C^[CT]CG[GA]G 2165 CCCGAG BalI 771 TGGCCA TGG^CCA 2333 TGGCCA BanI 2517 GGTACC G^G[CT][GA]CC 2565 GGTGCC BsaHI 538 GACGCC G[GA]^CG[CT]C 1356 GACGTC BsaI 1568 GAGACC GGTCTC(1/5) 2226 GAGACC BseRI 263 CTCCTC GAGGAG(10/8) 1342 GAGGAG BsmI 84 GCATTC GAATGC(1/-1) 1889 GCATTC BsoBI 1818 CCCGAG C^[CT]CG[GA]G 2165 CCCGAG BspMI 1484 ACCTGC ACCTGC(4/8) 1608 GCAGGT BstAPI 2170 GCAGCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2356 GCAGTGGCTGC BstXI 2030 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2384 CCAGGCTGGTGG EcoT22I 82 ATGCAT ATGCA^T 837 ATGCAT Hin1I 538 GACGCC G[GA]^CG[CT]C 1356 GACGTC MluNI 771 TGGCCA TGG^CCA 2333 TGGCCA MscI 771 TGGCCA TGG^CCA 2333 TGGCCA NcoI 774 CCATGG C^CATGG 2471 CCATGG NsiI 82 ATGCAT ATGCA^T 837 ATGCAT PvuII 295 CAGCTG CAG^CTG 628 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmnI