##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AspEI AspI AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV FauI FbaI FseI HaeII
HindIII HpaI KasI KspI MamI
MfeI MluI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 1356 GACGTC GACGT^C
Acc65I 2517 GGTACC G^GTACC
AccII 2046 CGCG CG^CG
AccIII 1914 TCCGGA T^CCGGA
AlwNI 2357 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2586 GGGCCC GGGCC^C
AseI 454 ATTAAT AT^TAAT
AsnI 454 ATTAAT AT^TAAT
Asp718I 2517 GGTACC G^GTACC
AspHI 856 GAGCAC G[AT]GC[AT]^C
AviII 1886 TGCGCA TGC^GCA
BcgI 1263 GCAACTATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1190 TACGTG [CT]AC^GT[GA]
BsaWI 1914 TCCGGA [AT]^CCGG[AT]
BseAI 1914 TCCGGA T^CCGGA
BsiEI 2544 CGGCCG CG[GA][CT]^CG
BsiHKAI 856 GAGCAC G[AT]GC[AT]^C
BsmBI 1378 CGTCTC CGTCTC(1/5)
BspEI 1914 TCCGGA T^CCGGA
BspHI 2250 TCATGA T^CATGA
BsrDI 998 GCAATG GCAATG(2/0)
BsrFI 2542 GCCGGC [GA]^CCGG[CT]
Bst1107I 678 GTATAC GTA^TAC
BstUI 2046 CGCG CG^CG
BstZ17I 678 GTATAC GTA^TAC
CfoI 1887 GCGC GCG^C
Cfr10I 2542 GCCGGC [GA]^CCGG[CT]
DraI 1783 TTTAAA TTT^AAA
DraIII 2560 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2469 GACCATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2544 CGGCCG C^GGCCG
EarI 318 GAAGAG CTCTTC(1/4)
EclXI 2544 CGGCCG C^GGCCG
Eco52I 2544 CGGCCG C^GGCCG
EcoNI 2590 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1886 TGCGCA TGC^GCA
HgaI 538 GACGC GACGC(5/10)
HhaI 1887 GCGC GCG^C
HinP1I 1887 GCGC G^CGC
HincII 1626 GTCAAC GT[CT]^[GA]AC
HindII 1626 GTCAAC GT[CT]^[GA]AC
KpnI 2517 GGTACC GGTAC^C
Ksp632I 318 GAAGAG CTCTTC(1/4)
MroI 1914 TCCGGA T^CCGGA
MvnI 2046 CGCG CG^CG
NaeI 2542 GCCGGC GCC^GGC
NdeI 1151 CATATG CA^TATG
NgoMIV 2542 GCCGGC G^CCGGC
NspI 1017 GCATGT [GA]CATG^[CT]
PflMI 2115 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2644 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 454 ATTAAT AT^TAAT
RcaI 2250 TCATGA T^CATGA
SapI 318 GAAGAGC GCTCTTC(1/4)
SexAI 2561 ACCTGGT A^CC[AT]GGT
SspI 1171 AATATT AAT^ATT
StuI 2579 AGGCCT AGG^CCT
TaqI 1452 TCGA T^CGA
TthHB8I 1452 TCGA T^CGA
Van91I 2115 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 678 GTATAC GT^[AC][GT]AC
1536 GTCTAC
AcyI 538 GACGCC G[GA]^CG[CT]C
1356 GACGTC
AvaI 1818 CCCGAG C^[CT]CG[GA]G
2165 CCCGAG
BalI 771 TGGCCA TGG^CCA
2333 TGGCCA
BanI 2517 GGTACC G^G[CT][GA]CC
2565 GGTGCC
BsaHI 538 GACGCC G[GA]^CG[CT]C
1356 GACGTC
BsaI 1568 GAGACC GGTCTC(1/5)
2226 GAGACC
BseRI 263 CTCCTC GAGGAG(10/8)
1342 GAGGAG
BsmI 84 GCATTC GAATGC(1/-1)
1889 GCATTC
BsoBI 1818 CCCGAG C^[CT]CG[GA]G
2165 CCCGAG
BspMI 1484 ACCTGC ACCTGC(4/8)
1608 GCAGGT
BstAPI 2170 GCAGCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2356 GCAGTGGCTGC
BstXI 2030 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2384 CCAGGCTGGTGG
EcoT22I 82 ATGCAT ATGCA^T
837 ATGCAT
Hin1I 538 GACGCC G[GA]^CG[CT]C
1356 GACGTC
MluNI 771 TGGCCA TGG^CCA
2333 TGGCCA
MscI 771 TGGCCA TGG^CCA
2333 TGGCCA
NcoI 774 CCATGG C^CATGG
2471 CCATGG
NsiI 82 ATGCAT ATGCA^T
837 ATGCAT
PvuII 295 CAGCTG CAG^CTG
628 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII XmnI