Restriction map for
pF1KB0067
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# NO CUT ENZYMES ( 42 enzymes) #
##################################
Acc65I AccIII AflII AscI Asp718I
AviII AvrII BamHI BfrI BglII
BlnI BsaBI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI FseI FspI HpaI KpnI
MamI MroI NdeI NheI NotI
NspV PmeI PvuI SacI SbfI
ScaI SfuI SnaBI SpeI Sse8387I
SwaI XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 3466 GACGTC GACGT^C
AclI 1168 AACGTT AA^CGTT
AgeI 1756 ACCGGT A^CCGGT
AseI 592 ATTAAT AT^TAAT
AsnI 592 ATTAAT AT^TAAT
BbrPI 5137 CACGTG CAC^GTG
BciVI 7097 GGATAC GTATCC(6/5)
BclI 4467 TGATCA T^GATCA
BsaWI 1756 ACCGGT [AT]^CCGG[AT]
BsiEI 6137 CGGCCG CG[GA][CT]^CG
Bsp1407I 4286 TGTACA T^GTACA
BsrGI 4286 TGTACA T^GTACA
BstEII 2775 GGTGACC G^GT[ACGT]ACC
BstPI 2775 GGTGACC G^GT[ACGT]ACC
CpoI 21 CGGACCG CG^G[AT]CCG
EagI 6137 CGGCCG C^GGCCG
EclXI 6137 CGGCCG C^GGCCG
Eco52I 6137 CGGCCG C^GGCCG
EcoO65I 2775 GGTGACC G^GT[ACGT]ACC
EcoT22I 3868 ATGCAT ATGCA^T
FbaI 4467 TGATCA T^GATCA
HindIII 3538 AAGCTT A^AGCTT
KspI 6448 CCGCGG CCGC^GG
MluI 5342 ACGCGT A^CGCGT
NruI 3666 TCGCGA TCG^CGA
NsiI 3868 ATGCAT ATGCA^T
PacI 589 TTAATTAA TTAAT^TAA
PaeR7I 702 CTCGAG C^TCGAG
PinAI 1756 ACCGGT A^CCGGT
PmaCI 5137 CACGTG CAC^GTG
PmlI 5137 CACGTG CAC^GTG
PshAI 2117 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 592 ATTAAT AT^TAAT
Psp1406I 1168 AACGTT AA^CGTT
RsrII 21 CGGACCG CG^G[AT]CCG
SacII 6448 CCGCGG CCGC^GG
SalI 4948 GTCGAC G^TCGAC
SspBI 4286 TGTACA T^GTACA
SspI 1946 AATATT AAT^ATT
XhoI 702 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 4948 GTCGAC GT^[AC][GT]AC
5110 GTCTAC
AocI 1504 CCTCAGG CC^T[ACGT]AGG
2512 CCTCAGG
Aor51HI 5318 AGCGCT AGC^GCT
6193 AGCGCT
Asp700I 2388 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3714 GAAGTGTTTC
BspHI 623 TCATGA T^CATGA
6518 TCATGA
BspLU11I 1782 ACATGT A^CATGT
1916 ACATGT
BsrDI 4453 CATTGC GCAATG(2/0)
7219 GCAATG
BssSI 4275 CACGAG CACGAG(-5/-1)
2222 CTCGTG
Bsu36I 1504 CCTCAGG CC^T[ACGT]AGG
2512 CCTCAGG
DraI 159 TTTAAA TTT^AAA
3253 TTTAAA
DraIII 5134 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
5160 CACTGTGTG
Eco47III 5318 AGCGCT AGC^GCT
6193 AGCGCT
Eco81I 1504 CCTCAGG CC^T[ACGT]AGG
2512 CCTCAGG
EcoNI 726 CCTAACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5574 CCTGCAGCAGG
EcoRV 937 GATATC GAT^ATC
6117 GATATC
MfeI 1016 CAATTG C^AATTG
1367 CAATTG
MunI 1016 CAATTG C^AATTG
1367 CAATTG
RcaI 623 TCATGA T^CATGA
6518 TCATGA
SfiI 292 GGCCTGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6129 GGCCCAGGCGGCC
SgrAI 1755 CACCGGTG C[GA]^CCGG[CT]G
5596 CGCCGGCG
XmnI 2388 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3714 GAAGTGTTTC
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# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspEI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI Eam1105I EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI