Restriction map for
pF1KB5272
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbsI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MluI MroI MslI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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ApaI 119 GGGCCC GGGCC^C
AvaI 122 CCCGGG C^[CT]CG[GA]G
BalI 209 TGGCCA TGG^CCA
BamHI 164 GGATCC G^GATCC
BanII 119 GGGCCC G[GA]GC[CT]^C
BclI 401 TGATCA T^GATCA
BsaI 598 GAGACC GGTCTC(1/5)
BsaJI 122 CCCGGG C^C[ACGT][ACGT]GG
BsoBI 122 CCCGGG C^[CT]CG[GA]G
Bsp1407I 697 TGTACA T^GTACA
BsrDI 351 CATTGC GCAATG(2/0)
BsrGI 697 TGTACA T^GTACA
BstAPI 61 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 707 GGATG GGATG(2/0)
EaeI 209 TGGCCA [CT]^GGCC[GA]
FauI 117 GCGGG CCCGC(4/6)
FbaI 401 TGATCA T^GATCA
FokI 707 GGATG GGATG(9/13)
MaeIII 490 GTCAC ^GT[ACGT]AC
MfeI 647 CAATTG C^AATTG
MluNI 209 TGGCCA TGG^CCA
MscI 209 TGGCCA TGG^CCA
MseI 295 TTAA T^TAA
MspA1I 169 CAGCTG C[AC]G^C[GT]G
MunI 647 CAATTG C^AATTG
PleI 451 GAGTC GAGTC(4/5)
PstI 49 CTGCAG CTGCA^G
PvuII 169 CAGCTG CAG^CTG
SapI 181 GAAGAGC GCTCTTC(1/4)
ScaI 530 AGTACT AGT^ACT
SfcI 49 CTGCAG C^T[GA][CT]AG
SmaI 122 CCCGGG CCC^GGG
SspBI 697 TGTACA T^GTACA
TaqI 20 TCGA T^CGA
TfiI 570 GATTC G^A[AT]TC
Tru9I 295 TTAA T^TAA
Tsp45I 490 GTCAC ^GT[GC]AC
Tsp509I 648 AATT ^AATT
TspRI 213 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 20 TCGA T^CGA
XmaCI 122 CCCGGG C^CCGGG
XmaI 122 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 531 GTAC GT^AC
698 GTAC
AlwNI 43 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
718 CAGACCCTG
AspHI 194 GTGCTC G[AT]GC[AT]^C
688 GAGCAC
AvaII 189 GGACC G^G[AT]CC
714 GGACC
BbvCI 133 GCTGAGG CCTCAGC(-5/-2)
139 GCTGAGG
BsiHKAI 194 GTGCTC G[AT]GC[AT]^C
688 GAGCAC
BsiYI 123 CCGGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
192 CCGTGCTCAGG
BslI 123 CCGGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
192 CCGTGCTCAGG
BstYI 38 AGATCC [GA]^GATC[CT]
164 GGATCC
HaeII 86 AGCGCC [GA]GCGC^[CT]
203 AGCGCC
HapII 123 CCGG C^CGG
288 CCGG
HinfI 451 GAGTC G^A[ACGT]TC
570 GATTC
HpaII 123 CCGG C^CGG
288 CCGG
MflI 38 AGATCC [GA]^GATC[CT]
164 GGATCC
MspI 123 CCGG C^CGG
288 CCGG
NlaIV 119 GGGCCC GG[ACGT]^[ACGT]CC
164 GGATCC
RsaI 531 GTAC GT^AC
698 GTAC
SfaNI 67 GATGC GCATC(5/9)
708 GATGC
XhoII 38 AGATCC [GA]^GATC[CT]
164 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BseRI
BsmAI Bsp1286I BssKI BstNI Cac8I
CelII CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI HaeIII HhaI
HinP1I HphI ItaI Ksp632I MboI
MboII MnlI MvaI MwoI NciI
NdeII Sau3AI Sau96I ScrFI SmlI
TseI