Restriction map for
pF1KE1015
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BseAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 45 ACGTGT A^C[GA][CT]GT
AgeI 560 ACCGGT A^CCGGT
Alw44I 231 GTGCAC G^TGCAC
ApaI 920 GGGCCC GGGCC^C
ApaLI 231 GTGCAC G^TGCAC
AspI 787 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 302 TGGCCA TGG^CCA
BbeI 894 GGCGCC GGCGC^C
BcgI 609 CGATGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 483 CACGTA [CT]AC^GT[GA]
BsaI 220 GAGACC GGTCTC(1/5)
BsgI 121 CTGCAC GTGCAG(16/14)
BsiEI 827 CGGCCG CG[GA][CT]^CG
BsmI 837 GAATGC GAATGC(1/-1)
BspMI 632 ACCTGC ACCTGC(4/8)
BsrDI 615 CATTGC GCAATG(2/0)
BsrFI 560 ACCGGT [GA]^CCGG[CT]
BssSI 164 CTCGTG CACGAG(-5/-1)
BstF5I 768 CATCC GGATG(2/0)
BstXI 866 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 895 GCGC GCG^C
Cfr10I 560 ACCGGT [GA]^CCGG[CT]
DrdI 784 GACGACAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 827 CGGCCG C^GGCCG
EclXI 827 CGGCCG C^GGCCG
Eco52I 827 CGGCCG C^GGCCG
EcoRV 7 GATATC GAT^ATC
FokI 768 CATCC GGATG(9/13)
HaeII 894 GGCGCC [GA]GCGC^[CT]
HhaI 895 GCGC GCG^C
HinP1I 895 GCGC G^CGC
HphI 903 GGTGA GGTGA(8/7)
KasI 894 GGCGCC G^GCGCC
MluNI 302 TGGCCA TGG^CCA
MscI 302 TGGCCA TGG^CCA
NarI 894 GGCGCC GG^CGCC
PflFI 787 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 560 ACCGGT A^CCGGT
PvuII 673 CAGCTG CAG^CTG
SapI 761 GCTCTTC GCTCTTC(1/4)
SfiI 819 GGCCCACGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 894 GGCGCC GGC^GCC
SmlI 625 CTCAAG C^T[CT][GA]AG
StuI 797 AGGCCT AGG^CCT
TaqI 731 TCGA T^CGA
Tth111I 787 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 731 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 415 GACGCC G[GA]^CG[CT]C
894 GGCGCC
BanI 139 GGCACC G^G[CT][GA]CC
894 GGCGCC
BsaHI 415 GACGCC G[GA]^CG[CT]C
894 GGCGCC
BsaWI 113 ACCGGA [AT]^CCGG[AT]
560 ACCGGT
BseRI 65 GAGGAG GAGGAG(10/8)
733 GAGGAG
BsmAI 220 GAGAC GTCTC(1/5)
311 GTCTC
BsmFI 91 GGGAC GGGAC(10/14)
657 GTCCC
BsrI 258 CCAGT ACTGG(1/-1)
714 CCAGT
DsaI 176 CCATGG C^C[GA][CT]GG
499 CCATGG
EaeI 302 TGGCCA [CT]^GGCC[GA]
827 CGGCCG
HgaI 117 GACGC GACGC(5/10)
415 GACGC
Hin1I 415 GACGCC G[GA]^CG[CT]C
894 GGCGCC
HinfI 20 GACTC G^A[ACGT]TC
309 GAGTC
MaeIII 128 GTGAC ^GT[ACGT]AC
269 GTGAC
MslI 321 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
453 CATGGAGATG
NcoI 176 CCATGG C^CATGG
499 CCATGG
NspI 279 GCATGT [GA]CATG^[CT]
579 GCATGT
PleI 20 GACTC GAGTC(4/5)
309 GAGTC
PstI 421 CTGCAG CTGCA^G
634 CTGCAG
SacI 70 GAGCTC GAGCT^C
181 GAGCTC
ScaI 602 AGTACT AGT^ACT
737 AGTACT
SfcI 421 CTGCAG C^T[GA][CT]AG
634 CTGCAG
Tsp45I 128 GTGAC ^GT[GC]AC
269 GTGAC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BamHI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstNI BstUI BstYI Cac8I Cfr13I
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HpaII ItaI Ksp632I MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
TspRI XhoII