Restriction map for
pF1KB9480
##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AflII AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AvrII BamHI BbrPI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsiWI BsmI
BspDI BspLU11I BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KpnI
KspI MamI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SspI SwaI Tru9I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 185 TCCGGA T^CCGGA
AcsI 1642 AAATTC [GA]^AATT[CT]
AflIII 1139 ACGTGT A^C[GA][CT]GT
AgeI 311 ACCGGT A^CCGGT
AhdI 2449 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 445 GTGCAC G^TGCAC
ApaLI 445 GTGCAC G^TGCAC
ApoI 1642 AAATTC [GA]^AATT[CT]
Asp700I 1128 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2449 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1221 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2031 CTCGGG C^[CT]CG[GA]G
BbeI 1696 GGCGCC GGCGC^C
BcgI 800 GCACAGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 929 TGATCA T^GATCA
BcnI 420 CCGGG CC^[GC]GG
BfaI 2116 CTAG C^TAG
BglI 1935 GCCCTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 185 TCCGGA T^CCGGA
BsiEI 1808 CGGCCG CG[GA][CT]^CG
BsmBI 322 CGTCTC CGTCTC(1/5)
BsoBI 2031 CTCGGG C^[CT]CG[GA]G
BspEI 185 TCCGGA T^CCGGA
BspHI 1901 TCATGA T^CATGA
BsrBI 874 GAGCGG CCGCTC(-3/-3)
BssHII 1801 GCGCGC G^CGCGC
BstAPI 99 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 1808 CGGCCG C^GGCCG
Eam1105I 2449 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1808 CGGCCG C^GGCCG
Eco52I 1808 CGGCCG C^GGCCG
EcoNI 183 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 929 TGATCA T^GATCA
KasI 1696 GGCGCC G^GCGCC
MaeI 2116 CTAG C^TAG
MroI 185 TCCGGA T^CCGGA
NaeI 2004 GCCGGC GCC^GGC
NarI 1696 GGCGCC GG^CGCC
NciI 420 CCGGG CC^[GC]GG
NgoMIV 2004 GCCGGC G^CCGGC
PflFI 1221 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2046 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 311 ACCGGT A^CCGGT
PpuMI 539 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1901 TCATGA T^CATGA
SacI 2370 GAGCTC GAGCT^C
SapI 2085 GCTCTTC GCTCTTC(1/4)
SbfI 96 CCTGCAGG CCTGCA^GG
ScaI 1001 AGTACT AGT^ACT
SfoI 1696 GGCGCC GGC^GCC
SphI 665 GCATGC GCATG^C
Sse8387I 96 CCTGCAGG CCTGCA^GG
Tsp509I 1643 AATT ^AATT
Tth111I 1221 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2046 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1128 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 1802 CGCG CG^CG
2058 CGCG
AviII 1757 TGCGCA TGC^GCA
2102 TGCGCA
BalI 17 TGGCCA TGG^CCA
948 TGGCCA
BanI 28 GGCACC G^G[CT][GA]CC
1696 GGCGCC
BbsI 1033 GAAGAC GAAGAC(2/6)
1888 GTCTTC
BbvCI 1724 CCTCAGC CCTCAGC(-5/-2)
1651 GCTGAGG
BpuAI 1033 GAAGAC GAAGAC(2/6)
1888 GTCTTC
BsaWI 185 TCCGGA [AT]^CCGG[AT]
311 ACCGGT
BseRI 1330 CTCCTC GAGGAG(10/8)
2410 GAGGAG
Bsp1407I 1778 TGTACA T^GTACA
1949 TGTACA
BsrFI 311 ACCGGT [GA]^CCGG[CT]
2004 GCCGGC
BsrGI 1778 TGTACA T^GTACA
1949 TGTACA
BstUI 1802 CGCG CG^CG
2058 CGCG
BstXI 125 CCACCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1391 CCAAGCCTGTGG
BstYI 416 AGATCC [GA]^GATC[CT]
2024 AGATCC
Cfr10I 311 ACCGGT [GA]^CCGG[CT]
2004 GCCGGC
DraII 101 AGGGCCT [GA]G^G[ACGT]CC[CT]
539 GGGACCT
EcoO109I 101 AGGGCCT [GA]G^G[ACGT]CC[CT]
539 GGGACCT
FspI 1757 TGCGCA TGC^GCA
2102 TGCGCA
HaeII 73 AGCGCC [GA]GCGC^[CT]
1696 GGCGCC
HincII 346 GTTGAC GT[CT]^[GA]AC
526 GTCAAC
HindII 346 GTTGAC GT[CT]^[GA]AC
526 GTCAAC
MflI 416 AGATCC [GA]^GATC[CT]
2024 AGATCC
MluNI 17 TGGCCA TGG^CCA
948 TGGCCA
MscI 17 TGGCCA TGG^CCA
948 TGGCCA
MspA1I 463 CAGCGG C[AC]G^C[GT]G
1623 CCGCTG
MvnI 1802 CGCG CG^CG
2058 CGCG
NcoI 1370 CCATGG C^CATGG
1745 CCATGG
NspI 665 GCATGC [GA]CATG^[CT]
1952 ACATGC
SexAI 1967 ACCTGGT A^CC[AT]GGT
2045 ACCAGGT
SspBI 1778 TGTACA T^GTACA
1949 TGTACA
StuI 1767 AGGCCT AGG^CCT
1841 AGGCCT
TfiI 737 GAATC G^A[AT]TC
2487 GATTC
XcmI 357 CCAAAGGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2178 CCACGTCTGCTTTGG
XhoII 416 AGATCC [GA]^GATC[CT]
2024 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanII
BbvI BmyI BpmI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I