Restriction map for
pF1KB9271
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI HgaI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NciI NcoI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfuI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
TseI Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 6 GGCGCC G[GA]^CG[CT]C
AfaI 99 GTAC GT^AC
AflII 492 CTTAAG C^TTAAG
AlwNI 193 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 635 GGGCCC GGGCC^C
AspHI 814 GAGCAC G[AT]GC[AT]^C
AvaI 917 CTCGAG C^[CT]CG[GA]G
AviII 235 TGCGCA TGC^GCA
BamHI 1580 GGATCC G^GATCC
BanII 635 GGGCCC G[GA]GC[CT]^C
BbeI 6 GGCGCC GGCGC^C
BbsI 34 GAAGAC GAAGAC(2/6)
BfaI 362 CTAG C^TAG
BfrI 492 CTTAAG C^TTAAG
BpuAI 34 GAAGAC GAAGAC(2/6)
BsaHI 6 GGCGCC G[GA]^CG[CT]C
BsaJI 1433 CCTTGG C^C[ACGT][ACGT]GG
BseRI 969 CTCCTC GAGGAG(10/8)
BsgI 1356 GTGCAG GTGCAG(16/14)
BsiHKAI 814 GAGCAC G[AT]GC[AT]^C
BsoBI 917 CTCGAG C^[CT]CG[GA]G
BsrFI 9 GCCGGC [GA]^CCGG[CT]
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
DraIII 1069 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 96 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1433 CCTTGG C^C[AT][AT]GG
Fnu4HI 4 GCGGC GC^[ACGT]GC
FspI 235 TGCGCA TGC^GCA
HaeII 6 GGCGCC [GA]GCGC^[CT]
Hin1I 6 GGCGCC G[GA]^CG[CT]C
HindIII 953 AAGCTT A^AGCTT
ItaI 4 GCGGC GC^[ACGT]GC
KasI 6 GGCGCC G^GCGCC
MaeI 362 CTAG C^TAG
MaeII 38 ACGT A^CGT
MspA1I 283 CAGCTG C[AC]G^C[GT]G
MwoI 4 GCGGCGCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 9 GCCGGC GCC^GGC
NarI 6 GGCGCC GG^CGCC
NgoMIV 9 GCCGGC G^CCGGC
NspI 598 ACATGC [GA]CATG^[CT]
PaeR7I 917 CTCGAG C^TCGAG
PstI 123 CTGCAG CTGCA^G
PvuII 283 CAGCTG CAG^CTG
RsaI 99 GTAC GT^AC
SbfI 122 CCTGCAGG CCTGCA^GG
SfoI 6 GGCGCC GGC^GCC
SgrAI 8 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 122 CCTGCAGG CCTGCA^GG
StyI 1433 CCTTGG C^C[AT][AT]GG
XhoI 917 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 386 AAATTC [GA]^AATT[CT]
1560 AAATTT
ApoI 386 AAATTC [GA]^AATT[CT]
1560 AAATTT
Asp700I 783 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1623 GAAAGCCTTC
BanI 6 GGCGCC G^G[CT][GA]CC
71 GGCACC
BsaI 118 GAGACC GGTCTC(1/5)
135 GGTCTC
BsaWI 832 ACCGGA [AT]^CCGG[AT]
1252 ACCGGA
BsmAI 118 GAGAC GTCTC(1/5)
136 GTCTC
BsrBI 882 CCGCTC CCGCTC(-3/-3)
1050 CCGCTC
BsrDI 686 GCAATG GCAATG(2/0)
1694 GCAATG
BstYI 331 AGATCC [GA]^GATC[CT]
1580 GGATCC
CfoI 7 GCGC GCG^C
236 GCGC
HhaI 7 GCGC GCG^C
236 GCGC
HinP1I 7 GCGC G^CGC
236 GCGC
MflI 331 AGATCC [GA]^GATC[CT]
1580 GGATCC
XhoII 331 AGATCC [GA]^GATC[CT]
1580 GGATCC
XmnI 783 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1623 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AvaII BmyI
BpmI BsiYI BslI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FokI HaeIII
HapII HinfI HpaII HphI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI NdeI NdeII
NlaIII NlaIV PleI PpuMI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I