Restriction map for
pF1KB9774
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AocI Aor51HI AscI AseI
AsnI Asp718I AspI AvrII BalI
BciVI BclI BfrI BlnI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 601 GTCGAC GT^[AC][GT]AC
AcsI 424 GAATTT [GA]^AATT[CT]
AflIII 1889 ACACGT A^C[GA][CT]GT
ApoI 424 GAATTT [GA]^AATT[CT]
Asp700I 1656 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1890 CACGTG CAC^GTG
BbvCI 28 CCTCAGC CCTCAGC(-5/-2)
BcgI 1571 GCAAGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1178 CTAG C^TAG
BglII 284 AGATCT A^GATCT
BlpI 754 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 754 GCTGAGC GC^T[ACGT]AGC
BsaAI 1890 CACGTG [CT]AC^GT[GA]
BsmAI 1068 GTCTC GTCTC(1/5)
BsmI 149 GAATGC GAATGC(1/-1)
BsrDI 606 CATTGC GCAATG(2/0)
BsrFI 120 GCCGGC [GA]^CCGG[CT]
BssSI 1554 CTCGTG CACGAG(-5/-1)
CelII 754 GCTGAGC GC^T[ACGT]AGC
Cfr10I 120 GCCGGC [GA]^CCGG[CT]
DraIII 888 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
KspI 1704 CCGCGG CCGC^GG
MaeI 1178 CTAG C^TAG
MaeII 1891 ACGT A^CGT
NaeI 120 GCCGGC GCC^GGC
NcoI 1126 CCATGG C^CATGG
NgoMIV 120 GCCGGC G^CCGGC
PflMI 45 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1890 CACGTG CAC^GTG
PmlI 1890 CACGTG CAC^GTG
PshAI 594 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1704 CCGCGG CCGC^GG
SalI 601 GTCGAC G^TCGAC
SapI 1656 GAAGAGC GCTCTTC(1/4)
TaqI 602 TCGA T^CGA
TfiI 940 GAATC G^A[AT]TC
TthHB8I 602 TCGA T^CGA
Van91I 45 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 311 CCATTCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1656 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 480 GTAC GT^AC
615 GTAC
AhdI 973 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1087 GACATTTTGTC
Alw44I 1811 GTGCAC G^TGCAC
1951 GTGCAC
ApaI 80 GGGCCC GGGCC^C
1968 GGGCCC
ApaLI 1811 GTGCAC G^TGCAC
1951 GTGCAC
AspEI 973 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1087 GACATTTTGTC
BamHI 819 GGATCC G^GATCC
1053 GGATCC
BglI 310 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1670 GCCACTCGGGC
BsgI 1528 CTGCAC GTGCAG(16/14)
1783 CTGCAC
BstAPI 1490 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1842 GCAGAACCTGC
BstXI 1120 CCATACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1295 CCATCGCGGTGG
EagI 19 CGGCCG C^GGCCG
416 CGGCCG
Eam1105I 973 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1087 GACATTTTGTC
EarI 799 GAAGAG CTCTTC(1/4)
1656 GAAGAG
EclXI 19 CGGCCG C^GGCCG
416 CGGCCG
Eco52I 19 CGGCCG C^GGCCG
416 CGGCCG
EcoNI 468 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
675 CCTTGTTAAGG
HincII 12 GTCAAC GT[CT]^[GA]AC
601 GTCGAC
HindII 12 GTCAAC GT[CT]^[GA]AC
601 GTCGAC
HphI 873 GGTGA GGTGA(8/7)
1197 GGTGA
Ksp632I 799 GAAGAG CTCTTC(1/4)
1656 GAAGAG
MaeIII 592 GTGAC ^GT[ACGT]AC
1181 GTGAC
MseI 680 TTAA T^TAA
989 TTAA
RsaI 480 GTAC GT^AC
615 GTAC
Tru9I 680 TTAA T^TAA
989 TTAA
Tsp45I 592 GTGAC ^GT[GC]AC
1181 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaI AvaII AviII
BanI BanII BbeI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TseI Tsp509I TspRI XhoII
XmaCI XmaI