Restriction map for
pF1KB0098
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AgeI AocI
Aor51HI AscI AseI AsnI AviII
AvrII BamHI BbvCI BclI BlnI
BlpI Bpu1102I BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoRI FbaI FspI
HgaI HindIII KspI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 173 GGTACC G^GTACC
AccI 2326 GTCTAC GT^[AC][GT]AC
AcyI 1482 GGCGCC G[GA]^CG[CT]C
AflII 1816 CTTAAG C^TTAAG
AflIII 1271 ACGTGT A^C[GA][CT]GT
AhdI 505 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1368 GTGCAC G^TGCAC
ApaLI 1368 GTGCAC G^TGCAC
Asp718I 173 GGTACC G^GTACC
AspEI 505 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 105 CCCGGG C^[CT]CG[GA]G
BbeI 1482 GGCGCC GGCGC^C
BbrPI 1270 CACGTG CAC^GTG
BcgI 2915 CGAGCATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 928 GGATAC GTATCC(6/5)
BfrI 1816 CTTAAG C^TTAAG
BglI 1319 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2817 AGATCT A^GATCT
BsaBI 831 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1482 GGCGCC G[GA]^CG[CT]C
BsaI 2898 GGTCTC GGTCTC(1/5)
BsaWI 1259 ACCGGA [AT]^CCGG[AT]
BsoBI 105 CCCGGG C^[CT]CG[GA]G
BspMI 2033 ACCTGC ACCTGC(4/8)
BsrFI 6 GCCGGC [GA]^CCGG[CT]
BstAPI 418 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 246 GGTGACC G^GT[ACGT]ACC
BstPI 246 GGTGACC G^GT[ACGT]ACC
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
DraI 1161 TTTAAA TTT^AAA
DrdI 2200 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 505 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 246 GGTGACC G^GT[ACGT]ACC
EcoRV 1263 GATATC GAT^ATC
EcoT22I 1607 ATGCAT ATGCA^T
FseI 5 GGCCGGCC GGCCGG^CC
HaeII 1482 GGCGCC [GA]GCGC^[CT]
Hin1I 1482 GGCGCC G[GA]^CG[CT]C
HpaI 712 GTTAAC GTT^AAC
KasI 1482 GGCGCC G^GCGCC
KpnI 173 GGTACC GGTAC^C
MamI 831 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 6 GCCGGC GCC^GGC
NarI 1482 GGCGCC GG^CGCC
NgoMIV 6 GCCGGC G^CCGGC
NsiI 1607 ATGCAT ATGCA^T
PmaCI 1270 CACGTG CAC^GTG
PmlI 1270 CACGTG CAC^GTG
PshAI 249 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 1482 GGCGCC GGC^GCC
SmaI 105 CCCGGG CCC^GGG
SphI 2918 GCATGC GCATG^C
SspI 1431 AATATT AAT^ATT
StuI 756 AGGCCT AGG^CCT
XcmI 1295 CCAAAGAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 105 CCCGGG C^CCGGG
XmaI 105 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 1496 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2626 CAGTTGCTG
ApaI 663 GGGCCC GGGCC^C
1408 GGGCCC
AspI 810 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2320 GACCGAGTC
BanI 173 GGTACC G^G[CT][GA]CC
1482 GGCGCC
BpmI 2508 CTGGAG CTGGAG(16/14)
2632 CTGGAG
BsaAI 1270 CACGTG [CT]AC^GT[GA]
1365 TACGTG
BsrDI 2274 CATTGC GCAATG(2/0)
2939 CATTGC
BstXI 1859 CCATGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2732 CCATCAGTGTGG
DsaI 2000 CCATGG C^C[GA][CT]GG
2442 CCATGG
HincII 679 GTCAAC GT[CT]^[GA]AC
712 GTTAAC
HindII 679 GTCAAC GT[CT]^[GA]AC
712 GTTAAC
NcoI 2000 CCATGG C^CATGG
2442 CCATGG
NspI 2186 GCATGT [GA]CATG^[CT]
2918 GCATGC
PflFI 810 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2320 GACCGAGTC
PvuII 1042 CAGCTG CAG^CTG
2849 CAGCTG
SmlI 1816 CTTAAG C^T[CT][GA]AG
1982 CTCAAG
Tth111I 810 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2320 GACCGAGTC
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# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI