Restriction map for
pF1KB3508
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 69 GAATTC [GA]^AATT[CT]
AfaI 294 GTAC GT^AC
AflIII 309 ACACGT A^C[GA][CT]GT
AlwNI 509 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 358 GGGCCC GGGCC^C
ApoI 69 GAATTC [GA]^AATT[CT]
BbsI 305 GAAGAC GAAGAC(2/6)
BciVI 313 GTATCC GTATCC(6/5)
BfaI 689 CTAG C^TAG
BglII 830 AGATCT A^GATCT
BlpI 807 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 807 GCTCAGC GC^T[ACGT]AGC
BpuAI 305 GAAGAC GAAGAC(2/6)
BsaAI 310 CACGTA [CT]AC^GT[GA]
BsaBI 714 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 466 GTGCAG GTGCAG(16/14)
BsmFI 286 GGGAC GGGAC(10/14)
Bsp1407I 293 TGTACA T^GTACA
BsrFI 377 ACCGGC [GA]^CCGG[CT]
BsrGI 293 TGTACA T^GTACA
BstAPI 792 GCATTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 803 GCCTGC GC[ACGT]^[ACGT]GC
CelII 807 GCTCAGC GC^T[ACGT]AGC
CfoI 801 GCGC GCG^C
Cfr10I 377 ACCGGC [GA]^CCGG[CT]
DraII 357 AGGGCCC [GA]G^G[ACGT]CC[CT]
EarI 251 CTCTTC CTCTTC(1/4)
EcoNI 324 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 357 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 69 GAATTC G^AATTC
EcoT14I 420 CCAAGG C^C[AT][AT]GG
HapII 378 CCGG C^CGG
HhaI 801 GCGC GCG^C
HinP1I 801 GCGC G^CGC
HpaII 378 CCGG C^CGG
Ksp632I 251 CTCTTC CTCTTC(1/4)
MaeI 689 CTAG C^TAG
MaeII 311 ACGT A^CGT
MamI 714 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 882 CCGCTG C[AC]G^C[GT]G
MspI 378 CCGG C^CGG
PflMI 375 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 522 GAGTC GAGTC(4/5)
RsaI 294 GTAC GT^AC
SfcI 649 CTACAG C^T[GA][CT]AG
SmlI 979 CTCAAG C^T[CT][GA]AG
SspBI 293 TGTACA T^GTACA
StyI 420 CCAAGG C^C[AT][AT]GG
Van91I 375 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 882 CCGC CCGC(-3/-1)
891 CCGC
AlwI 390 GATCC GGATC(4/5)
749 GGATC
AspHI 334 GAGCAC G[AT]GC[AT]^C
430 GAGCAC
AvaII 114 GGTCC G^G[AT]CC
287 GGACC
BanII 358 GGGCCC G[GA]GC[CT]^C
941 GAGCCC
BpmI 353 CTGGAG CTGGAG(16/14)
382 CTGGAG
BsaI 214 GAGACC GGTCTC(1/5)
682 GAGACC
BsaJI 117 CCAGGG C^C[ACGT][ACGT]GG
420 CCAAGG
BsiHKAI 334 GAGCAC G[AT]GC[AT]^C
430 GAGCAC
BsmI 593 GAATGC GAATGC(1/-1)
792 GCATTC
BstYI 389 AGATCC [GA]^GATC[CT]
830 AGATCT
EcoT22I 638 ATGCAT ATGCA^T
790 ATGCAT
HincII 205 GTCAAC GT[CT]^[GA]AC
343 GTCAAC
HindII 205 GTCAAC GT[CT]^[GA]AC
343 GTCAAC
HindIII 123 AAGCTT A^AGCTT
949 AAGCTT
HphI 156 GGTGA GGTGA(8/7)
73 TCACC
MflI 389 AGATCC [GA]^GATC[CT]
830 AGATCT
MseI 587 TTAA T^TAA
659 TTAA
NsiI 638 ATGCAT ATGCA^T
790 ATGCAT
StuI 449 AGGCCT AGG^CCT
859 AGGCCT
TfiI 171 GAATC G^A[AT]TC
568 GATTC
Tru9I 587 TTAA T^TAA
659 TTAA
Tsp509I 40 AATT ^AATT
70 AATT
TspRI 282 CAGTG CA[GC]TG[ACGT][ACGT]^
300 CACTG
XhoII 389 AGATCC [GA]^GATC[CT]
830 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI BbvI BmyI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TthHB8I