Restriction map for
pF1KB3654
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BssHII Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI FspI HgaI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NciI
NcoI NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SmaI SmlI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 1421 GGCGCC G[GA]^CG[CT]C
ApaI 702 GGGCCC GGGCC^C
AseI 606 ATTAAT AT^TAAT
AsnI 606 ATTAAT AT^TAAT
BalI 2838 TGGCCA TGG^CCA
BanI 1421 GGCGCC G^G[CT][GA]CC
BanII 702 GGGCCC G[GA]GC[CT]^C
BbeI 1421 GGCGCC GGCGC^C
BbvCI 541 CCTCAGC CCTCAGC(-5/-2)
BsaAI 2565 TACGTA [CT]AC^GT[GA]
BsaHI 1421 GGCGCC G[GA]^CG[CT]C
BsaWI 2269 ACCGGA [AT]^CCGG[AT]
BsrBI 2487 CCGCTC CCGCTC(-3/-3)
BsrDI 2459 GCAATG GCAATG(2/0)
BssSI 2436 CACGAG CACGAG(-5/-1)
BstBI 1727 TTCGAA TT^CGAA
BstXI 312 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1422 GCGC GCG^C
EaeI 2838 TGGCCA [CT]^GGCC[GA]
EcoT22I 1243 ATGCAT ATGCA^T
FauI 498 CCCGC CCCGC(4/6)
HaeII 1421 GGCGCC [GA]GCGC^[CT]
HhaI 1422 GCGC GCG^C
Hin1I 1421 GGCGCC G[GA]^CG[CT]C
HinP1I 1422 GCGC G^CGC
KasI 1421 GGCGCC G^GCGCC
MluNI 2838 TGGCCA TGG^CCA
MscI 2838 TGGCCA TGG^CCA
MspA1I 485 CAGCTG C[AC]G^C[GT]G
NaeI 182 GCCGGC GCC^GGC
NarI 1421 GGCGCC GG^CGCC
NdeI 2114 CATATG CA^TATG
NgoMIV 182 GCCGGC G^CCGGC
NsiI 1243 ATGCAT ATGCA^T
NspI 694 GCATGT [GA]CATG^[CT]
NspV 1727 TTCGAA TT^CGAA
PpuMI 878 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2728 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 606 ATTAAT AT^TAAT
PstI 358 CTGCAG CTGCA^G
PvuII 485 CAGCTG CAG^CTG
SfoI 1421 GGCGCC GGC^GCC
SfuI 1727 TTCGAA TT^CGAA
SgrAI 202 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 2565 TACGTA TAC^GTA
StuI 1401 AGGCCT AGG^CCT
XcmI 150 CCATGAGACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 499 CCGC CCGC(-3/-1)
2487 CCGC
AlwNI 238 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
265 CAGTTTCTG
BmyI 437 GTGCCC G[AGT]GC[ACT]^C
702 GGGCCC
BsgI 1579 CTGCAC GTGCAG(16/14)
1682 GTGCAG
Bsp1286I 437 GTGCCC G[AGT]GC[ACT]^C
702 GGGCCC
BsrFI 182 GCCGGC [GA]^CCGG[CT]
203 GCCGGT
Cfr10I 182 GCCGGC [GA]^CCGG[CT]
203 GCCGGT
EcoT14I 90 CCAAGG C^C[AT][AT]GG
230 CCAAGG
HindIII 566 AAGCTT A^AGCTT
1550 AAGCTT
MaeII 144 ACGT A^CGT
2566 ACGT
PflMI 423 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
639 CCAGGAGTTGG
SfcI 358 CTGCAG C^T[GA][CT]AG
1389 CTATAG
StyI 90 CCAAGG C^C[AT][AT]GG
230 CCAAGG
Van91I 423 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
639 CCAGGAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BamHI BbsI BbvI
BfaI BpmI BpuAI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmI
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI