Restriction map for
pF1KB0410
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BciVI BfrI
BlnI BsaAI BsaBI BsaHI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I KasI KpnI MamI MluI
MroI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SfoI SfuI
SgrAI SmlI SnaBI SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Aor51HI 130 AGCGCT AGC^GCT
BclI 1490 TGATCA T^GATCA
BglI 10 GCCGACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1567 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1567 GCTCAGC GC^T[ACGT]AGC
BsaWI 2312 TCCGGT [AT]^CCGG[AT]
BsmFI 806 GGGAC GGGAC(10/14)
BsrFI 165 GCCGGC [GA]^CCGG[CT]
BstAPI 1899 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1883 CCAAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1567 GCTCAGC GC^T[ACGT]AGC
Cfr10I 165 GCCGGC [GA]^CCGG[CT]
DraI 1641 TTTAAA TTT^AAA
DraIII 226 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 412 GACCCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 130 AGCGCT AGC^GCT
FbaI 1490 TGATCA T^GATCA
HaeII 130 AGCGCT [GA]GCGC^[CT]
HgaI 112 GACGC GACGC(5/10)
HpaI 1510 GTTAAC GTT^AAC
NaeI 165 GCCGGC GCC^GGC
NgoMIV 165 GCCGGC G^CCGGC
NspI 731 GCATGC [GA]CATG^[CT]
PflMI 297 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 738 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 268 GAGCTC GAGCT^C
SbfI 1905 CCTGCAGG CCTGCA^GG
SfiI 9 GGCCGACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1821 ACTAGT A^CTAGT
SphI 731 GCATGC GCATG^C
Sse8387I 1905 CCTGCAGG CCTGCA^GG
Van91I 297 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 44 enzymes) #
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AcsI 394 AAATTC [GA]^AATT[CT]
1022 AAATTC
Alw44I 283 GTGCAC G^TGCAC
1111 GTGCAC
AlwI 2404 GATCC GGATC(4/5)
2099 GGATC
AlwNI 298 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1570 CAGCTCCTG
AocI 457 CCTGAGG CC^T[ACGT]AGG
1740 CCTGAGG
ApaI 28 GGGCCC GGGCC^C
124 GGGCCC
ApaLI 283 GTGCAC G^TGCAC
1111 GTGCAC
ApoI 394 AAATTC [GA]^AATT[CT]
1022 AAATTC
BalI 1466 TGGCCA TGG^CCA
2298 TGGCCA
BanI 324 GGTGCC G^G[CT][GA]CC
536 GGTGCC
BbsI 1205 GTCTTC GAAGAC(2/6)
2461 GTCTTC
BcgI 39 CGACGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
966 GCACAATAATCG
BglII 629 AGATCT A^GATCT
2255 AGATCT
BpuAI 1205 GTCTTC GAAGAC(2/6)
2461 GTCTTC
BsaI 652 GAGACC GGTCTC(1/5)
506 GGTCTC
BsgI 2089 CTGCAC GTGCAG(16/14)
2177 CTGCAC
BsiEI 8 CGGCCG CG[GA][CT]^CG
167 CGGCCG
BsmI 799 GAATGC GAATGC(1/-1)
136 GCATTC
BsrBI 334 CCGCTC CCGCTC(-3/-3)
192 GAGCGG
BsrDI 2379 CATTGC GCAATG(2/0)
1844 GCAATG
Bsu36I 457 CCTGAGG CC^T[ACGT]AGG
1740 CCTGAGG
CfoI 49 GCGC GCG^C
131 GCGC
EagI 8 CGGCCG C^GGCCG
167 CGGCCG
EclXI 8 CGGCCG C^GGCCG
167 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
167 CGGCCG
Eco81I 457 CCTGAGG CC^T[ACGT]AGG
1740 CCTGAGG
HhaI 49 GCGC GCG^C
131 GCGC
HinP1I 49 GCGC G^CGC
131 GCGC
HincII 616 GTTGAC GT[CT]^[GA]AC
1510 GTTAAC
HindII 616 GTTGAC GT[CT]^[GA]AC
1510 GTTAAC
HindIII 1676 AAGCTT A^AGCTT
1729 AAGCTT
KspI 32 CCGCGG CCGC^GG
2127 CCGCGG
MfeI 914 CAATTG C^AATTG
1274 CAATTG
MluNI 1466 TGGCCA TGG^CCA
2298 TGGCCA
MscI 1466 TGGCCA TGG^CCA
2298 TGGCCA
MslI 255 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
318 CACCTCGGTG
MunI 914 CAATTG C^AATTG
1274 CAATTG
NcoI 512 CCATGG C^CATGG
557 CCATGG
PpuMI 299 AGGACCT [GA]G^G[AT]CC[CT]
410 AGGACCC
SacII 32 CCGCGG CCGC^GG
2127 CCGCGG
SexAI 302 ACCTGGT A^CC[AT]GGT
1523 ACCAGGT
StuI 1257 AGGCCT AGG^CCT
1902 AGGCCT
TaqI 975 TCGA T^CGA
2027 TCGA
TthHB8I 975 TCGA T^CGA
2027 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmaCI
XmaI