Restriction map for
pF1KA1560
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I CfoI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FseI FspI HaeII HhaI HinP1I
KasI KpnI MamI MluI MluNI
MroI MscI NaeI NarI NciI
NdeI NgoMIV NotI NruI PacI
PaeR7I PflFI PmaCI PmeI PmlI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1017 GTCTAC GT^[AC][GT]AC
AccII 304 CGCG CG^CG
AcyI 320 GACGCC G[GA]^CG[CT]C
AgeI 1248 ACCGGT A^CCGGT
AseI 1345 ATTAAT AT^TAAT
AsnI 1345 ATTAAT AT^TAAT
AvaI 980 CTCGGG C^[CT]CG[GA]G
BanI 1888 GGCACC G^G[CT][GA]CC
BciVI 245 GTATCC GTATCC(6/5)
BsaHI 320 GACGCC G[GA]^CG[CT]C
BsaI 2380 GAGACC GGTCTC(1/5)
BseRI 1474 CTCCTC GAGGAG(10/8)
BsmBI 655 GAGACG CGTCTC(1/5)
BsoBI 980 CTCGGG C^[CT]CG[GA]G
BssSI 1553 CTCGTG CACGAG(-5/-1)
BstBI 1699 TTCGAA TT^CGAA
BstUI 304 CGCG CG^CG
BstXI 804 CCATAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 144 TTTAAA TTT^AAA
DraII 1800 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1456 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1793 GACTGGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1856 CGGCCA [CT]^GGCC[GA]
EarI 727 CTCTTC CTCTTC(1/4)
EcoO109I 1800 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1996 GATATC GAT^ATC
EcoT22I 1600 ATGCAT ATGCA^T
HgaI 320 GACGC GACGC(5/10)
Hin1I 320 GACGCC G[GA]^CG[CT]C
HpaI 487 GTTAAC GTT^AAC
Ksp632I 727 CTCTTC CTCTTC(1/4)
KspI 303 CCGCGG CCGC^GG
MfeI 425 CAATTG C^AATTG
MunI 425 CAATTG C^AATTG
MvnI 304 CGCG CG^CG
NcoI 888 CCATGG C^CATGG
NheI 1426 GCTAGC G^CTAGC
NsiI 1600 ATGCAT ATGCA^T
NspV 1699 TTCGAA TT^CGAA
PflMI 181 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1248 ACCGGT A^CCGGT
PpuMI 1800 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 989 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1345 ATTAAT AT^TAAT
SacII 303 CCGCGG CCGC^GG
SfaNI 1315 GATGC GCATC(5/9)
SfuI 1699 TTCGAA TT^CGAA
SmlI 631 CTTGAG C^T[CT][GA]AG
SphI 1602 GCATGC GCATG^C
SspI 1223 AATATT AAT^ATT
StuI 2189 AGGCCT AGG^CCT
Van91I 181 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2454 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 949 ACACGT A^C[GA][CT]GT
1337 ACACGT
AlwI 692 GATCC GGATC(4/5)
801 GATCC
AlwNI 1612 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2240 CAGAACCTG
Asp700I 904 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2458 GAATATATTC
AvaII 1801 GGTCC G^G[AT]CC
2230 GGTCC
BclI 554 TGATCA T^GATCA
1826 TGATCA
BglII 133 AGATCT A^GATCT
932 AGATCT
BlpI 1405 GCTGAGC GC^T[ACGT]AGC
2014 GCTGAGC
BpmI 1270 CTGGAG CTGGAG(16/14)
2185 CTGGAG
Bpu1102I 1405 GCTGAGC GC^T[ACGT]AGC
2014 GCTGAGC
BsaWI 1248 ACCGGT [AT]^CCGG[AT]
1952 TCCGGT
BsgI 11 CTGCAC GTGCAG(16/14)
207 CTGCAC
BsmI 2340 GAATGC GAATGC(1/-1)
1961 GCATTC
BsrFI 546 ACCGGC [GA]^CCGG[CT]
1248 ACCGGT
CelII 1405 GCTGAGC GC^T[ACGT]AGC
2014 GCTGAGC
Cfr10I 546 ACCGGC [GA]^CCGG[CT]
1248 ACCGGT
DsaI 303 CCGCGG C^C[GA][CT]GG
888 CCATGG
FbaI 554 TGATCA T^GATCA
1826 TGATCA
HincII 487 GTTAAC GT[CT]^[GA]AC
626 GTCAAC
HindII 487 GTTAAC GT[CT]^[GA]AC
626 GTCAAC
HindIII 808 AAGCTT A^AGCTT
2035 AAGCTT
NspI 368 GCATGT [GA]CATG^[CT]
1602 GCATGC
PvuII 1612 CAGCTG CAG^CTG
1840 CAGCTG
XcmI 1451 CCACACACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1607 CCATACAGCTGCTGG
XmnI 904 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2458 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII