Restriction map for
pF1KB7338
##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BamHI BbrPI BbvCI
BfrI BglII BlnI BsaAI BsiWI
BsmI BspDI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoT22I
FseI HindIII KpnI MfeI MluI
MluNI MscI MunI NaeI NcoI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 2724 ACATGT A^C[GA][CT]GT
AgeI 1452 ACCGGT A^CCGGT
ApaI 2861 GGGCCC GGGCC^C
Asp700I 2551 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 309 TGCGCA TGC^GCA
BclI 683 TGATCA T^GATCA
BlpI 2667 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2667 GCTCAGC GC^T[ACGT]AGC
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmBI 1141 GAGACG CGTCTC(1/5)
BspLU11I 2724 ACATGT A^CATGT
BsrFI 1452 ACCGGT [GA]^CCGG[CT]
BstXI 2162 CCAGGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1124 AGATCC [GA]^GATC[CT]
CelII 2667 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1452 ACCGGT [GA]^CCGG[CT]
DraI 1837 TTTAAA TTT^AAA
EaeI 71 CGGCCG [CT]^GGCC[GA]
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
EcoNI 1225 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 683 TGATCA T^GATCA
FspI 309 TGCGCA TGC^GCA
HincII 988 GTTAAC GT[CT]^[GA]AC
HindII 988 GTTAAC GT[CT]^[GA]AC
HpaI 988 GTTAAC GTT^AAC
HphI 2004 TCACC GGTGA(8/7)
MflI 1124 AGATCC [GA]^GATC[CT]
NdeI 2048 CATATG CA^TATG
NotI 70 GCGGCCGC GC^GGCCGC
PflMI 395 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1452 ACCGGT A^CCGGT
PstI 2593 CTGCAG CTGCA^G
PvuII 3160 CAGCTG CAG^CTG
SapI 1668 GCTCTTC GCTCTTC(1/4)
SmlI 2245 CTTGAG C^T[CT][GA]AG
StuI 1845 AGGCCT AGG^CCT
Van91I 395 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1124 AGATCC [GA]^GATC[CT]
XmnI 2551 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 579 TCCGGA T^CCGGA
1342 TCCGGA
AlwNI 809 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1474 CAGCTTCTG
BbeI 39 GGCGCC GGCGC^C
228 GGCGCC
BbsI 1927 GAAGAC GAAGAC(2/6)
1699 GTCTTC
BcgI 161 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2264 GCATTAACTTCG
BciVI 802 GGATAC GTATCC(6/5)
1198 GGATAC
BfaI 1964 CTAG C^TAG
3049 CTAG
BglI 103 GCCTGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
152 GCCCCAAGGGC
BpuAI 1927 GAAGAC GAAGAC(2/6)
1699 GTCTTC
BseAI 579 TCCGGA T^CCGGA
1342 TCCGGA
BspEI 579 TCCGGA T^CCGGA
1342 TCCGGA
BspMI 508 ACCTGC ACCTGC(4/8)
2355 GCAGGT
KasI 39 GGCGCC G^GCGCC
228 GGCGCC
MaeI 1964 CTAG C^TAG
3049 CTAG
MroI 579 TCCGGA T^CCGGA
1342 TCCGGA
NarI 39 GGCGCC GG^CGCC
228 GGCGCC
NspI 1329 ACATGC [GA]CATG^[CT]
2724 ACATGT
PleI 1531 GACTC GAGTC(4/5)
2813 GACTC
SacI 1098 GAGCTC GAGCT^C
2665 GAGCTC
SfoI 39 GGCGCC GGC^GCC
228 GGCGCC
SspI 1863 AATATT AAT^ATT
2485 AATATT
XcmI 1122 CCAGATCCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2513 CCATCCTGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoO109I EcoRI EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI Ksp632I KspI
MaeII MaeIII MamI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI