Restriction map for pF1KB9655
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
ApaLI       AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BalI        BbrPI       
BbsI        BbvCI       BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        DraI        DraIII      
Eam1105I    EarI        Eco81I      EcoO65I     EcoRI       
EcoRV       FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     MfeI        
MluI        MluNI       MscI        MunI        NdeI        
NheI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SalI        
SapI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspBI       StuI        
SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AatII         229  GACGTC               GACGT^C
AccIII        867  TCCGGA               T^CCGGA
Aor51HI      1775  AGCGCT               AGC^GCT
AscI         1429  GGCGCGCC             GG^CGCGCC
AspI          262  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI         502  GGATAC               GTATCC(6/5)
BclI          671  TGATCA               T^GATCA
BsaWI         867  TCCGGA               [AT]^CCGG[AT]
BseAI         867  TCCGGA               T^CCGGA
BsmBI        1846  GAGACG               CGTCTC(1/5)
BspEI         867  TCCGGA               T^CCGGA
BstAPI       1251  GCAGATCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI          177  GACGAATCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1775  AGCGCT               AGC^GCT
EcoNI        1743  CCTGGGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       862  ATGCAT               ATGCA^T
FbaI          671  TGATCA               T^GATCA
KspI         1706  CCGCGG               CCGC^GG
MboII        1995  GAAGA                GAAGA(8/7)
MroI          867  TCCGGA               T^CCGGA
NcoI         1277  CCATGG               C^CATGG
NotI         1708  GCGGCCGC             GC^GGCCGC
NsiI          862  ATGCAT               ATGCA^T
PflFI         262  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         782  CCAATCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          557  GAGCTC               GAGCT^C
SacII        1706  CCGCGG               CCGC^GG
SbfI         1293  CCTGCAGG             CCTGCA^GG
ScaI         1655  AGTACT               AGT^ACT
SmlI         1960  CTCAAG               C^T[CT][GA]AG
Sse8387I     1293  CCTGCAGG             CCTGCA^GG
SspI          751  AATATT               AAT^ATT
Tth111I       262  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        782  CCAATCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI           46  AAATTT               [GA]^AATT[CT]
              646  AAATTT              
ApaI         1265  GGGCCC               GGGCC^C
             1353  GGGCCC              
ApoI           46  AAATTT               [GA]^AATT[CT]
              646  AAATTT              
AspHI          10  GAGCAC               G[AT]GC[AT]^C
              557  GAGCTC              
BamHI          85  GGATCC               G^GATCC
              777  GGATCC              
BcgI          291  CGATGGCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              843  GCAAGGAAATCG        
BfaI          425  CTAG                 C^TAG
             1049  CTAG                
BsaBI         669  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              778  GATCCCAATC          
BsaI          267  GGTCTC               GGTCTC(1/5)
              727  GGTCTC              
BsgI         1590  CTGCAC               GTGCAG(16/14)
             1066  GTGCAG              
BsiHKAI        10  GAGCAC               G[AT]GC[AT]^C
              557  GAGCTC              
BsrBI        1603  CCGCTC               CCGCTC(-3/-3)
             1957  CCGCTC              
BssHII       1430  GCGCGC               G^CGCGC
             1717  GCGCGC              
CpoI          390  CGGACCG              CG^G[AT]CCG
             1370  CGGACCG             
MaeI          425  CTAG                 C^TAG
             1049  CTAG                
MaeII         230  ACGT                 A^CGT
             1072  ACGT                
MamI          669  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              778  GATCCCAATC          
MslI          834  CACCAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1932  CACGCCCGTG          
NspI          695  GCATGT               [GA]CATG^[CT]
             1011  GCATGT              
PpuMI        1421  AGGACCC              [GA]G^G[AT]CC[CT]
             1820  AGGACCT             
PvuII           4  CAGCTG               CAG^CTG
             1836  CAGCTG              
RsrII         390  CGGACCG              CG^G[AT]CCG
             1370  CGGACCG             
SfaNI         897  GATGC                GCATC(5/9)
              864  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaI        AvaII       BanI        BanII       
BbeI        BbvI        BcnI        BglI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiEI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MaeIII      MboI        
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        SfoI        SmaI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmaCI       XmaI        
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