Restriction map for
pF1KB9655
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# NO CUT ENZYMES ( 99 enzymes) #
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Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaLI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BbrPI
BbsI BbvCI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI DraI DraIII
Eam1105I EarI Eco81I EcoO65I EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I MfeI
MluI MluNI MscI MunI NdeI
NheI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI StuI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 229 GACGTC GACGT^C
AccIII 867 TCCGGA T^CCGGA
Aor51HI 1775 AGCGCT AGC^GCT
AscI 1429 GGCGCGCC GG^CGCGCC
AspI 262 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 502 GGATAC GTATCC(6/5)
BclI 671 TGATCA T^GATCA
BsaWI 867 TCCGGA [AT]^CCGG[AT]
BseAI 867 TCCGGA T^CCGGA
BsmBI 1846 GAGACG CGTCTC(1/5)
BspEI 867 TCCGGA T^CCGGA
BstAPI 1251 GCAGATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 177 GACGAATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1775 AGCGCT AGC^GCT
EcoNI 1743 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 862 ATGCAT ATGCA^T
FbaI 671 TGATCA T^GATCA
KspI 1706 CCGCGG CCGC^GG
MboII 1995 GAAGA GAAGA(8/7)
MroI 867 TCCGGA T^CCGGA
NcoI 1277 CCATGG C^CATGG
NotI 1708 GCGGCCGC GC^GGCCGC
NsiI 862 ATGCAT ATGCA^T
PflFI 262 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 782 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 557 GAGCTC GAGCT^C
SacII 1706 CCGCGG CCGC^GG
SbfI 1293 CCTGCAGG CCTGCA^GG
ScaI 1655 AGTACT AGT^ACT
SmlI 1960 CTCAAG C^T[CT][GA]AG
Sse8387I 1293 CCTGCAGG CCTGCA^GG
SspI 751 AATATT AAT^ATT
Tth111I 262 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 782 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 46 AAATTT [GA]^AATT[CT]
646 AAATTT
ApaI 1265 GGGCCC GGGCC^C
1353 GGGCCC
ApoI 46 AAATTT [GA]^AATT[CT]
646 AAATTT
AspHI 10 GAGCAC G[AT]GC[AT]^C
557 GAGCTC
BamHI 85 GGATCC G^GATCC
777 GGATCC
BcgI 291 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
843 GCAAGGAAATCG
BfaI 425 CTAG C^TAG
1049 CTAG
BsaBI 669 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
778 GATCCCAATC
BsaI 267 GGTCTC GGTCTC(1/5)
727 GGTCTC
BsgI 1590 CTGCAC GTGCAG(16/14)
1066 GTGCAG
BsiHKAI 10 GAGCAC G[AT]GC[AT]^C
557 GAGCTC
BsrBI 1603 CCGCTC CCGCTC(-3/-3)
1957 CCGCTC
BssHII 1430 GCGCGC G^CGCGC
1717 GCGCGC
CpoI 390 CGGACCG CG^G[AT]CCG
1370 CGGACCG
MaeI 425 CTAG C^TAG
1049 CTAG
MaeII 230 ACGT A^CGT
1072 ACGT
MamI 669 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
778 GATCCCAATC
MslI 834 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1932 CACGCCCGTG
NspI 695 GCATGT [GA]CATG^[CT]
1011 GCATGT
PpuMI 1421 AGGACCC [GA]G^G[AT]CC[CT]
1820 AGGACCT
PvuII 4 CAGCTG CAG^CTG
1836 CAGCTG
RsrII 390 CGGACCG CG^G[AT]CCG
1370 CGGACCG
SfaNI 897 GATGC GCATC(5/9)
864 GCATC
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeIII MboI
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfcI SfoI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI