################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BbrPI BbsI BbvCI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI DraI DraIII Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MfeI MluI MluNI MscI MunI NdeI NheI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SalI SapI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 229 GACGTC GACGT^C AccIII 867 TCCGGA T^CCGGA Aor51HI 1775 AGCGCT AGC^GCT AscI 1429 GGCGCGCC GG^CGCGCC AspI 262 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 502 GGATAC GTATCC(6/5) BclI 671 TGATCA T^GATCA BsaWI 867 TCCGGA [AT]^CCGG[AT] BseAI 867 TCCGGA T^CCGGA BsmBI 1846 GAGACG CGTCTC(1/5) BspEI 867 TCCGGA T^CCGGA BstAPI 1251 GCAGATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 177 GACGAATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1775 AGCGCT AGC^GCT EcoNI 1743 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 862 ATGCAT ATGCA^T FbaI 671 TGATCA T^GATCA KspI 1706 CCGCGG CCGC^GG MboII 1995 GAAGA GAAGA(8/7) MroI 867 TCCGGA T^CCGGA NcoI 1277 CCATGG C^CATGG NotI 1708 GCGGCCGC GC^GGCCGC NsiI 862 ATGCAT ATGCA^T PflFI 262 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 782 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 557 GAGCTC GAGCT^C SacII 1706 CCGCGG CCGC^GG SbfI 1293 CCTGCAGG CCTGCA^GG ScaI 1655 AGTACT AGT^ACT SmlI 1960 CTCAAG C^T[CT][GA]AG Sse8387I 1293 CCTGCAGG CCTGCA^GG SspI 751 AATATT AAT^ATT Tth111I 262 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 782 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 46 AAATTT [GA]^AATT[CT] 646 AAATTT ApaI 1265 GGGCCC GGGCC^C 1353 GGGCCC ApoI 46 AAATTT [GA]^AATT[CT] 646 AAATTT AspHI 10 GAGCAC G[AT]GC[AT]^C 557 GAGCTC BamHI 85 GGATCC G^GATCC 777 GGATCC BcgI 291 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 843 GCAAGGAAATCG BfaI 425 CTAG C^TAG 1049 CTAG BsaBI 669 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 778 GATCCCAATC BsaI 267 GGTCTC GGTCTC(1/5) 727 GGTCTC BsgI 1590 CTGCAC GTGCAG(16/14) 1066 GTGCAG BsiHKAI 10 GAGCAC G[AT]GC[AT]^C 557 GAGCTC BsrBI 1603 CCGCTC CCGCTC(-3/-3) 1957 CCGCTC BssHII 1430 GCGCGC G^CGCGC 1717 GCGCGC CpoI 390 CGGACCG CG^G[AT]CCG 1370 CGGACCG MaeI 425 CTAG C^TAG 1049 CTAG MaeII 230 ACGT A^CGT 1072 ACGT MamI 669 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 778 GATCCCAATC MslI 834 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1932 CACGCCCGTG NspI 695 GCATGT [GA]CATG^[CT] 1011 GCATGT PpuMI 1421 AGGACCC [GA]G^G[AT]CC[CT] 1820 AGGACCT PvuII 4 CAGCTG CAG^CTG 1836 CAGCTG RsrII 390 CGGACCG CG^G[AT]CCG 1370 CGGACCG SfaNI 897 GATGC GCATC(5/9) 864 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeIII MboI MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI SfoI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI