Restriction map for
pF1KB6696
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AviII AvrII BbeI BbrPI BcgI
BfrI BlnI BpmI BsaAI BsaHI
BsaWI BseAI BsiWI BsmBI BsmI
BsoBI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I DraI DraII DraIII
DrdI Eco81I EcoNI EcoO109I EcoO65I
EcoRV FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI SacI
SacII SalI SbfI ScaI SfcI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AcsI 1286 GAATTC [GA]^AATT[CT]
AflIII 641 ACATGT A^C[GA][CT]GT
AhdI 369 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 196 AGCGCT AGC^GCT
ApoI 1286 GAATTC [GA]^AATT[CT]
AspEI 369 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 589 TGGCCA TGG^CCA
BamHI 767 GGATCC G^GATCC
BanI 163 GGTGCC G^G[CT][GA]CC
BanII 436 GGGCTC G[GA]GC[CT]^C
BbvCI 109 GCTGAGG CCTCAGC(-5/-2)
BciVI 1244 GTATCC GTATCC(6/5)
BclI 386 TGATCA T^GATCA
BglI 166 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 890 AGATCT A^GATCT
BlpI 10 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 10 GCTCAGC GC^T[ACGT]AGC
BsaBI 615 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1304 GAGACC GGTCTC(1/5)
BseRI 37 CTCCTC GAGGAG(10/8)
BsgI 666 CTGCAC GTGCAG(16/14)
Bsp1407I 841 TGTACA T^GTACA
BspDI 683 ATCGAT AT^CGAT
BspLU11I 641 ACATGT A^CATGT
BspMI 881 ACCTGC ACCTGC(4/8)
BsrFI 370 ACCGGC [GA]^CCGG[CT]
BsrGI 841 TGTACA T^GTACA
BstAPI 1366 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1016 TTCGAA TT^CGAA
CelII 10 GCTCAGC GC^T[ACGT]AGC
Cfr10I 370 ACCGGC [GA]^CCGG[CT]
ClaI 683 ATCGAT AT^CGAT
CpoI 321 CGGTCCG CG^G[AT]CCG
EagI 173 CGGCCG C^GGCCG
Eam1105I 369 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 173 CGGCCG C^GGCCG
Eco47III 196 AGCGCT AGC^GCT
Eco52I 173 CGGCCG C^GGCCG
EcoRI 1286 GAATTC G^AATTC
EcoT22I 1199 ATGCAT ATGCA^T
FbaI 386 TGATCA T^GATCA
HgaI 33 GACGC GACGC(5/10)
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
MamI 615 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 219 CAATTG C^AATTG
MluNI 589 TGGCCA TGG^CCA
MscI 589 TGGCCA TGG^CCA
MseI 1097 TTAA T^TAA
MunI 219 CAATTG C^AATTG
NcoI 185 CCATGG C^CATGG
NsiI 1199 ATGCAT ATGCA^T
NspV 1016 TTCGAA TT^CGAA
PleI 1071 GACTC GAGTC(4/5)
PvuII 1176 CAGCTG CAG^CTG
RsrII 321 CGGTCCG CG^G[AT]CCG
SapI 438 GCTCTTC GCTCTTC(1/4)
SexAI 865 ACCTGGT A^CC[AT]GGT
SfuI 1016 TTCGAA TT^CGAA
SphI 980 GCATGC GCATG^C
SspBI 841 TGTACA T^GTACA
StuI 713 AGGCCT AGG^CCT
TfiI 1011 GAATC G^A[AT]TC
Tru9I 1097 TTAA T^TAA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 79 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1367 CAGAAACTG
AspHI 139 GTGCTC G[AT]GC[AT]^C
925 GTGCTC
AvaII 19 GGTCC G^G[AT]CC
322 GGTCC
BbsI 489 GAAGAC GAAGAC(2/6)
788 GTCTTC
BcnI 171 CCCGG CC^[GC]GG
268 CCCGG
BpuAI 489 GAAGAC GAAGAC(2/6)
788 GTCTTC
BsiEI 173 CGGCCG CG[GA][CT]^CG
368 CGACCG
BsiHKAI 139 GTGCTC G[AT]GC[AT]^C
925 GTGCTC
BsrDI 690 CATTGC GCAATG(2/0)
1155 CATTGC
BstXI 291 CCACTTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1322 CCAATACAGTGG
BstYI 767 GGATCC [GA]^GATC[CT]
890 AGATCT
DsaI 185 CCATGG C^C[GA][CT]GG
1316 CCACGG
FauI 167 CCCGC CCCGC(4/6)
344 GCGGG
HaeII 114 GGCGCT [GA]GCGC^[CT]
196 AGCGCT
HinfI 1011 GAATC G^A[ACGT]TC
1071 GACTC
MaeII 1088 ACGT A^CGT
1126 ACGT
MaeIII 1108 GTTAC ^GT[ACGT]AC
1166 GTTAC
MflI 767 GGATCC [GA]^GATC[CT]
890 AGATCT
NciI 171 CCCGG CC^[GC]GG
268 CCCGG
NspI 641 ACATGT [GA]CATG^[CT]
980 GCATGC
SmlI 243 CTCAAG C^T[CT][GA]AG
967 CTCAAG
XhoII 767 GGATCC [GA]^GATC[CT]
890 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeI MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TseI Tsp509I TspRI TthHB8I XcmI