Restriction map for
pF1KE1962
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BanI BbeI BbrPI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 60 CGCG CG^CG
AclI 1315 AACGTT AA^CGTT
Asp700I 868 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 538 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1142 CTCGAG C^[CT]CG[GA]G
BalI 1148 TGGCCA TGG^CCA
BbsI 315 GAAGAC GAAGAC(2/6)
BbvCI 979 GCTGAGG CCTCAGC(-5/-2)
BcgI 1172 CGATGTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 135 GCCCCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 315 GAAGAC GAAGAC(2/6)
BsgI 1537 GTGCAG GTGCAG(16/14)
BsoBI 1142 CTCGAG C^[CT]CG[GA]G
BspHI 848 TCATGA T^CATGA
BsrFI 1390 GCCGGC [GA]^CCGG[CT]
BssSI 386 CTCGTG CACGAG(-5/-1)
BstEII 94 GGTAACC G^GT[ACGT]ACC
BstPI 94 GGTAACC G^GT[ACGT]ACC
BstUI 60 CGCG CG^CG
CfoI 61 GCGC GCG^C
Cfr10I 1390 GCCGGC [GA]^CCGG[CT]
DsaI 138 CCGTGG C^C[GA][CT]GG
EarI 1484 CTCTTC CTCTTC(1/4)
EcoO65I 94 GGTAACC G^GT[ACGT]ACC
EcoRI 203 GAATTC G^AATTC
HhaI 61 GCGC GCG^C
HinP1I 61 GCGC G^CGC
Ksp632I 1484 CTCTTC CTCTTC(1/4)
MluNI 1148 TGGCCA TGG^CCA
MscI 1148 TGGCCA TGG^CCA
MvnI 60 CGCG CG^CG
NaeI 1390 GCCGGC GCC^GGC
NgoMIV 1390 GCCGGC G^CCGGC
PaeR7I 1142 CTCGAG C^TCGAG
PflFI 538 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1315 AACGTT AA^CGTT
PvuII 365 CAGCTG CAG^CTG
RcaI 848 TCATGA T^CATGA
SapI 1483 GCTCTTC GCTCTTC(1/4)
SfiI 134 GGCCCCGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1142 CTCGAG C^T[CT][GA]AG
SspI 1271 AATATT AAT^ATT
Tth111I 538 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1142 CTCGAG C^TCGAG
XmnI 868 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 819 GTAC GT^AC
1497 GTAC
ApaI 133 GGGCCC GGGCC^C
142 GGGCCC
BanII 133 GGGCCC G[GA]GC[CT]^C
142 GGGCCC
BbvI 36 GCAGC GCAGC(8/12)
51 GCAGC
BcnI 12 CCGGG CC^[GC]GG
786 CCGGG
BsaBI 619 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
641 GATATGGATC
BsmI 1518 GAATGC GAATGC(1/-1)
1333 GCATTC
BsrDI 804 CATTGC GCAATG(2/0)
1386 CATTGC
BstXI 630 CCACATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1269 CCAATATTCTGG
EaeI 1148 TGGCCA [CT]^GGCC[GA]
1392 CGGCCA
EcoT14I 284 CCAAGG C^C[AT][AT]GG
899 CCAAGG
HphI 896 TCACC GGTGA(8/7)
1083 TCACC
MamI 619 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
641 GATATGGATC
MslI 631 CACATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1530 CACCTTTGTG
MspA1I 52 CAGCGG C[AC]G^C[GT]G
365 CAGCTG
NciI 12 CCGGG CC^[GC]GG
786 CCGGG
PflMI 899 CCAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1089 CCATCGCCTGG
PleI 318 GACTC GAGTC(4/5)
571 GAGTC
PpuMI 380 GGGACCC [GA]G^G[AT]CC[CT]
1098 GGGACCC
RsaI 819 GTAC GT^AC
1497 GTAC
SphI 222 GCATGC GCATG^C
1178 GCATGC
StyI 284 CCAAGG C^C[AT][AT]GG
899 CCAAGG
TseI 36 GCAGC G^C[AT]GC
51 GCAGC
Tsp45I 259 GTCAC ^GT[GC]AC
1163 GTGAC
Van91I 899 CCAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1089 CCATCGCCTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccIII AciI AcsI AluI AlwI
ApoI AspHI AvaII BamHI BmyI
BpmI BsaJI BsaWI BseAI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspEI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I Tsp509I TspRI
TthHB8I XhoII