Restriction map for
pF1KE1590
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AvrII
BcgI BciVI BfrI BglI BlnI
BsaBI BsiWI Bsp1407I BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoT22I
FseI HpaI KpnI KspI MamI
MfeI MluI MunI NcoI NheI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SwaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 1018 TCCGGA T^CCGGA
AlwNI 2191 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2110 GGGCCC GGGCC^C
AspI 361 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 695 TGCGCA TGC^GCA
BalI 885 TGGCCA TGG^CCA
BanI 1143 GGCGCC G^G[CT][GA]CC
BbeI 1143 GGCGCC GGCGC^C
BbrPI 982 CACGTG CAC^GTG
BclI 405 TGATCA T^GATCA
BglII 1504 AGATCT A^GATCT
BsaWI 1018 TCCGGA [AT]^CCGG[AT]
BseAI 1018 TCCGGA T^CCGGA
BsmBI 1891 GAGACG CGTCTC(1/5)
BspDI 3205 ATCGAT AT^CGAT
BspEI 1018 TCCGGA T^CCGGA
BsrBI 3403 GAGCGG CCGCTC(-3/-3)
ClaI 3205 ATCGAT AT^CGAT
DsaI 3494 CCACGG C^C[GA][CT]GG
EagI 534 CGGCCG C^GGCCG
EclXI 534 CGGCCG C^GGCCG
Eco52I 534 CGGCCG C^GGCCG
EcoRI 1215 GAATTC G^AATTC
FbaI 405 TGATCA T^GATCA
FspI 695 TGCGCA TGC^GCA
HindIII 3164 AAGCTT A^AGCTT
KasI 1143 GGCGCC G^GCGCC
MluNI 885 TGGCCA TGG^CCA
MroI 1018 TCCGGA T^CCGGA
MscI 885 TGGCCA TGG^CCA
NaeI 567 GCCGGC GCC^GGC
NarI 1143 GGCGCC GG^CGCC
NdeI 2707 CATATG CA^TATG
NgoMIV 567 GCCGGC G^CCGGC
NotI 533 GCGGCCGC GC^GGCCGC
NspI 2535 GCATGC [GA]CATG^[CT]
PaeR7I 2820 CTCGAG C^TCGAG
PflFI 361 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 982 CACGTG CAC^GTG
PmlI 982 CACGTG CAC^GTG
SbfI 1560 CCTGCAGG CCTGCA^GG
SfoI 1143 GGCGCC GGC^GCC
SphI 2535 GCATGC GCATG^C
Sse8387I 1560 CCTGCAGG CCTGCA^GG
Tth111I 361 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2646 TCTAGA T^CTAGA
XcmI 3092 CCAACCTTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2820 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccII 538 CGCG CG^CG
657 CGCG
AcyI 1143 GGCGCC G[GA]^CG[CT]C
1893 GACGCC
Alw44I 1059 GTGCAC G^TGCAC
3477 GTGCAC
ApaLI 1059 GTGCAC G^TGCAC
3477 GTGCAC
Asp700I 1211 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1291 GAAGAGGTTC
BamHI 255 GGATCC G^GATCC
3320 GGATCC
BbvCI 292 CCTCAGC CCTCAGC(-5/-2)
133 GCTGAGG
BlpI 2331 GCTTAGC GC^T[ACGT]AGC
2440 GCTGAGC
Bpu1102I 2331 GCTTAGC GC^T[ACGT]AGC
2440 GCTGAGC
BsaAI 982 CACGTG [CT]AC^GT[GA]
1048 TACGTG
BsaHI 1143 GGCGCC G[GA]^CG[CT]C
1893 GACGCC
BspMI 1827 ACCTGC ACCTGC(4/8)
3303 GCAGGT
BsrFI 371 ACCGGC [GA]^CCGG[CT]
567 GCCGGC
BssSI 2872 CACGAG CACGAG(-5/-1)
2150 CTCGTG
BstUI 538 CGCG CG^CG
657 CGCG
BstXI 672 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1574 CCATCAGTGTGG
CelII 2331 GCTTAGC GC^T[ACGT]AGC
2440 GCTGAGC
Cfr10I 371 ACCGGC [GA]^CCGG[CT]
567 GCCGGC
DraI 2728 TTTAAA TTT^AAA
2995 TTTAAA
DraIII 1454 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1876 CACTCAGTG
EcoRV 850 GATATC GAT^ATC
954 GATATC
HaeII 1143 GGCGCC [GA]GCGC^[CT]
2261 AGCGCC
HgaI 361 GACGC GACGC(5/10)
1893 GACGC
Hin1I 1143 GGCGCC G[GA]^CG[CT]C
1893 GACGCC
HincII 245 GTCAAC GT[CT]^[GA]AC
3067 GTTGAC
HindII 245 GTCAAC GT[CT]^[GA]AC
3067 GTTGAC
MvnI 538 CGCG CG^CG
657 CGCG
PflMI 2652 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3231 CCAAAGAATGG
PpuMI 1700 AGGACCT [GA]G^G[AT]CC[CT]
1837 GGGTCCC
SacI 1671 GAGCTC GAGCT^C
2155 GAGCTC
SspI 832 AATATT AAT^ATT
1435 AATATT
Van91I 2652 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3231 CCAAAGAATGG
XmnI 1211 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1291 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI