Restriction map for
pF1KE0299
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# NO CUT ENZYMES ( 48 enzymes) #
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Acc65I AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AviII AvrII BbeI BbrPI
BfrI BlnI BsaWI BseAI BsiWI
BspEI BssHII CpoI FseI FspI
KasI KpnI KspI MluI MroI
NarI NruI PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SfiI SfoI
SgrAI SnaBI SwaI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AatII 4874 GACGTC GACGT^C
AccII 105 CGCG CG^CG
Aor51HI 9689 AGCGCT AGC^GCT
ApaI 2882 GGGCCC GGGCC^C
BsaAI 5889 CACGTA [CT]AC^GT[GA]
Bsp1407I 10335 TGTACA T^GTACA
BspDI 8933 ATCGAT AT^CGAT
BsrBI 9823 GAGCGG CCGCTC(-3/-3)
BsrGI 10335 TGTACA T^GTACA
BstUI 105 CGCG CG^CG
ClaI 8933 ATCGAT AT^CGAT
Eco47III 9689 AGCGCT AGC^GCT
HaeII 9689 AGCGCT [GA]GCGC^[CT]
MvnI 105 CGCG CG^CG
NaeI 115 GCCGGC GCC^GGC
NgoMIV 115 GCCGGC G^CCGGC
NheI 76 GCTAGC G^CTAGC
NotI 52 GCGGCCGC GC^GGCCGC
PshAI 8385 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 15271 CCTGCAGG CCTGCA^GG
SmaI 13572 CCCGGG CCC^GGG
Sse8387I 15271 CCTGCAGG CCTGCA^GG
SspBI 10335 TGTACA T^GTACA
XmaCI 13572 CCCGGG C^CCGGG
XmaI 13572 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 308 GGCGTC G[GA]^CG[CT]C
4874 GACGTC
AhdI 7095 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9126 GACTACAGGTC
AspEI 7095 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9126 GACTACAGGTC
BanI 1296 GGCACC G^G[CT][GA]CC
2088 GGCACC
BglI 55 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1002 GCCCTCAGGGC
BsaHI 308 GGCGTC G[GA]^CG[CT]C
4874 GACGTC
BspLU11I 11501 ACATGT A^CATGT
14277 ACATGT
Bst1107I 2978 GTATAC GTA^TAC
11097 GTATAC
BstEII 2621 GGTCACC G^GT[ACGT]ACC
3564 GGTAACC
BstPI 2621 GGTCACC G^GT[ACGT]ACC
3564 GGTAACC
BstZ17I 2978 GTATAC GTA^TAC
11097 GTATAC
CfoI 187 GCGC GCG^C
9690 GCGC
DrdI 12255 GACACGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
13415 GACCACTCAGTC
Eam1105I 7095 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9126 GACTACAGGTC
EcoO65I 2621 GGTCACC G^GT[ACGT]ACC
3564 GGTAACC
HhaI 187 GCGC GCG^C
9690 GCGC
Hin1I 308 GGCGTC G[GA]^CG[CT]C
4874 GACGTC
HinP1I 187 GCGC G^CGC
9690 GCGC
HpaI 478 GTTAAC GTT^AAC
7326 GTTAAC
NdeI 8945 CATATG CA^TATG
13703 CATATG
PaeR7I 13235 CTCGAG C^TCGAG
14165 CTCGAG
SphI 5160 GCATGC GCATG^C
8490 GCATGC
XhoI 13235 CTCGAG C^TCGAG
14165 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BamHI BanII BbsI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstBI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI NspV
PflFI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScaI ScrFI SexAI
SfaNI SfcI SfuI SmlI SpeI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoII XmnI