Restriction map for
pF1KE0698
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AvrII BamHI BcgI BciVI
BclI BfrI BglI BlnI BsiEI
BsiHKAI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI HindIII HpaI KpnI KspI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 887 TCCGGA T^CCGGA
AcyI 787 GGCGCC G[GA]^CG[CT]C
AflIII 1182 ACGTGT A^C[GA][CT]GT
AocI 201 CCTTAGG CC^T[ACGT]AGG
AspI 2028 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 177 CTCGGG C^[CT]CG[GA]G
AviII 855 TGCGCA TGC^GCA
BbeI 787 GGCGCC GGCGC^C
BbrPI 1181 CACGTG CAC^GTG
BbvCI 1675 CCTCAGC CCTCAGC(-5/-2)
BcnI 1934 CCCGG CC^[GC]GG
BglII 1063 AGATCT A^GATCT
BlpI 970 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 970 GCTCAGC GC^T[ACGT]AGC
BsaHI 787 GGCGCC G[GA]^CG[CT]C
BsaI 1319 GAGACC GGTCTC(1/5)
BseAI 887 TCCGGA T^CCGGA
BsgI 907 CTGCAC GTGCAG(16/14)
BsoBI 177 CTCGGG C^[CT]CG[GA]G
BspEI 887 TCCGGA T^CCGGA
BspMI 905 ACCTGC ACCTGC(4/8)
BsrDI 341 CATTGC GCAATG(2/0)
Bsu36I 201 CCTTAGG CC^T[ACGT]AGG
CelII 970 GCTCAGC GC^T[ACGT]AGC
DraI 168 TTTAAA TTT^AAA
Eco81I 201 CCTTAGG CC^T[ACGT]AGG
EcoNI 1224 CCTCCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 855 TGCGCA TGC^GCA
HaeII 787 GGCGCC [GA]GCGC^[CT]
Hin1I 787 GGCGCC G[GA]^CG[CT]C
HincII 949 GTCAAC GT[CT]^[GA]AC
HindII 949 GTCAAC GT[CT]^[GA]AC
KasI 787 GGCGCC G^GCGCC
MfeI 943 CAATTG C^AATTG
MroI 887 TCCGGA T^CCGGA
MunI 943 CAATTG C^AATTG
NarI 787 GGCGCC GG^CGCC
NciI 1934 CCCGG CC^[GC]GG
NspI 282 GCATGC [GA]CATG^[CT]
PflFI 2028 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 954 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1181 CACGTG CAC^GTG
PmlI 1181 CACGTG CAC^GTG
SapI 2239 GAAGAGC GCTCTTC(1/4)
ScaI 664 AGTACT AGT^ACT
SfcI 452 CTACAG C^T[GA][CT]AG
SfoI 787 GGCGCC GGC^GCC
SphI 282 GCATGC GCATG^C
Tth111I 2028 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 954 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 1138 CGCG CG^CG
1716 CGCG
AseI 1480 ATTAAT AT^TAAT
2269 ATTAAT
AsnI 1480 ATTAAT AT^TAAT
2269 ATTAAT
BalI 614 TGGCCA TGG^CCA
807 TGGCCA
BanI 787 GGCGCC G^G[CT][GA]CC
1821 GGCACC
BanII 111 GAGCCC G[GA]GC[CT]^C
916 GGGCTC
BsaAI 430 TACGTG [CT]AC^GT[GA]
1181 CACGTG
BsaBI 475 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
595 GATATGAATC
BseRI 277 GAGGAG GAGGAG(10/8)
2164 GAGGAG
BsmI 570 GAATGC GAATGC(1/-1)
1356 GAATGC
BstUI 1138 CGCG CG^CG
1716 CGCG
BstYI 1063 AGATCT [GA]^GATC[CT]
1608 AGATCC
CfoI 788 GCGC GCG^C
856 GCGC
FauI 92 CCCGC CCCGC(4/6)
114 CCCGC
HhaI 788 GCGC GCG^C
856 GCGC
HinP1I 788 GCGC G^CGC
856 GCGC
MamI 475 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
595 GATATGAATC
MflI 1063 AGATCT [GA]^GATC[CT]
1608 AGATCC
MluNI 614 TGGCCA TGG^CCA
807 TGGCCA
MscI 614 TGGCCA TGG^CCA
807 TGGCCA
MvnI 1138 CGCG CG^CG
1716 CGCG
PleI 2028 GACTC GAGTC(4/5)
1906 GAGTC
PpuMI 1238 AGGACCC [GA]G^G[AT]CC[CT]
1374 GGGTCCT
PshBI 1480 ATTAAT AT^TAAT
2269 ATTAAT
SmlI 1535 CTCAAG C^T[CT][GA]AG
2098 CTCAAG
Tsp45I 433 GTGAC ^GT[GC]AC
490 GTCAC
XhoII 1063 AGATCT [GA]^GATC[CT]
1608 AGATCC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I