Restriction map for
pF1KB3168
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FseI FspI
KasI KpnI KspI MaeI MluI
MluNI MscI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI PvuII
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 1703 CGCG CG^CG
AclI 1487 AACGTT AA^CGTT
AcyI 1753 GACGCC G[GA]^CG[CT]C
AflIII 332 ACATGT A^C[GA][CT]GT
AlwNI 875 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 951 GGACC G^G[AT]CC
BamHI 1548 GGATCC G^GATCC
BanI 1987 GGCACC G^G[CT][GA]CC
BbvCI 1650 CCTCAGC CCTCAGC(-5/-2)
BciVI 2014 GGATAC GTATCC(6/5)
BclI 2301 TGATCA T^GATCA
BsaBI 927 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1753 GACGCC G[GA]^CG[CT]C
BspHI 2058 TCATGA T^CATGA
BspLU11I 332 ACATGT A^CATGT
BsrDI 2312 GCAATG GCAATG(2/0)
BsrFI 1828 GCCGGC [GA]^CCGG[CT]
BstEII 780 GGTGACC G^GT[ACGT]ACC
BstPI 780 GGTGACC G^GT[ACGT]ACC
BstUI 1703 CGCG CG^CG
BstXI 1737 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1828 GCCGGC [GA]^CCGG[CT]
DraIII 1872 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2077 GAAGAG CTCTTC(1/4)
EcoNI 15 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 780 GGTGACC G^GT[ACGT]ACC
EcoT22I 313 ATGCAT ATGCA^T
FbaI 2301 TGATCA T^GATCA
Hin1I 1753 GACGCC G[GA]^CG[CT]C
HincII 826 GTTAAC GT[CT]^[GA]AC
HindII 826 GTTAAC GT[CT]^[GA]AC
HindIII 271 AAGCTT A^AGCTT
HpaI 826 GTTAAC GTT^AAC
Ksp632I 2077 GAAGAG CTCTTC(1/4)
MaeII 1488 ACGT A^CGT
MamI 927 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 303 CAATTG C^AATTG
MspA1I 420 CCGCTG C[AC]G^C[GT]G
MunI 303 CAATTG C^AATTG
MvnI 1703 CGCG CG^CG
NaeI 1828 GCCGGC GCC^GGC
NgoMIV 1828 GCCGGC G^CCGGC
NsiI 313 ATGCAT ATGCA^T
PflMI 2220 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1487 AACGTT AA^CGTT
PstI 1605 CTGCAG CTGCA^G
RcaI 2058 TCATGA T^CATGA
SacI 2191 GAGCTC GAGCT^C
SapI 2077 GAAGAGC GCTCTTC(1/4)
SfaNI 2211 GCATC GCATC(5/9)
SphI 315 GCATGC GCATG^C
StuI 1637 AGGCCT AGG^CCT
Van91I 2220 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 266 TCCGGA T^CCGGA
1712 TCCGGA
AspHI 1218 GTGCTC G[AT]GC[AT]^C
2191 GAGCTC
BcgI 1752 CGACGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1295 GCATGGCGCTCG
BsaI 833 GAGACC GGTCTC(1/5)
1414 GAGACC
BsaWI 266 TCCGGA [AT]^CCGG[AT]
1712 TCCGGA
BseAI 266 TCCGGA T^CCGGA
1712 TCCGGA
BsiHKAI 1218 GTGCTC G[AT]GC[AT]^C
2191 GAGCTC
BspEI 266 TCCGGA T^CCGGA
1712 TCCGGA
BstAPI 1607 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2336 GCAACGAATGC
FauI 1725 CCCGC CCCGC(4/6)
2293 CCCGC
HgaI 1753 GACGC GACGC(5/10)
1942 GCGTC
MroI 266 TCCGGA T^CCGGA
1712 TCCGGA
NspI 315 GCATGC [GA]CATG^[CT]
332 ACATGT
ScaI 914 AGTACT AGT^ACT
1609 AGTACT
SmaI 1552 CCCGGG CCC^GGG
2150 CCCGGG
XcmI 1961 CCATTCCGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2216 CCAACCAGAGGATGG
XmaCI 1552 CCCGGG C^CCGGG
2150 CCCGGG
XmaI 1552 CCCGGG C^CCGGG
2150 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApaI ApoI Asp700I AvaI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI