Restriction map for
pF1KB9123
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII AvrII
BbeI BbrPI BclI BcnI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HapII HgaI Hin1I
HindIII HpaI HpaII KasI KpnI
MamI MfeI MluI MroI MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 32 CGCG CG^CG
AcsI 385 AAATTT [GA]^AATT[CT]
AfaI 1026 GTAC GT^AC
AflIII 639 ACATGT A^C[GA][CT]GT
ApoI 385 AAATTT [GA]^AATT[CT]
AseI 1410 ATTAAT AT^TAAT
AsnI 1410 ATTAAT AT^TAAT
Asp700I 699 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 117 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1356 TGGCCA TGG^CCA
BanI 1113 GGCACC G^G[CT][GA]CC
BbvCI 1055 CCTCAGC CCTCAGC(-5/-2)
BcgI 1269 GCAGAACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1331 GTATCC GTATCC(6/5)
BglII 220 AGATCT A^GATCT
BlpI 1249 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1249 GCTCAGC GC^T[ACGT]AGC
BspLU11I 639 ACATGT A^CATGT
BspMI 1380 ACCTGC ACCTGC(4/8)
BstBI 389 TTCGAA TT^CGAA
BstEII 39 GGTCACC G^GT[ACGT]ACC
BstPI 39 GGTCACC G^GT[ACGT]ACC
BstUI 32 CGCG CG^CG
BstXI 627 CCAGCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1249 GCTCAGC GC^T[ACGT]AGC
CfoI 923 GCGC GCG^C
EcoO65I 39 GGTCACC G^GT[ACGT]ACC
HaeII 922 GGCGCT [GA]GCGC^[CT]
HhaI 923 GCGC GCG^C
HinP1I 923 GCGC G^CGC
KspI 31 CCGCGG CCGC^GG
MaeII 47 ACGT A^CGT
MluNI 1356 TGGCCA TGG^CCA
MscI 1356 TGGCCA TGG^CCA
MslI 42 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 32 CGCG CG^CG
NcoI 473 CCATGG C^CATGG
NspI 639 ACATGT [GA]CATG^[CT]
NspV 389 TTCGAA TT^CGAA
PflFI 117 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1410 ATTAAT AT^TAAT
PstI 1205 CTGCAG CTGCA^G
PvuII 956 CAGCTG CAG^CTG
RsaI 1026 GTAC GT^AC
SacII 31 CCGCGG CCGC^GG
SalI 419 GTCGAC G^TCGAC
SfuI 389 TTCGAA TT^CGAA
SspI 237 AATATT AAT^ATT
Tth111I 117 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 699 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 419 GTCGAC GT^[AC][GT]AC
1365 GTCTAC
BamHI 651 GGATCC G^GATCC
684 GGATCC
BanII 3 GAGCTC G[GA]GC[CT]^C
947 GAGCTC
BbsI 1510 GAAGAC GAAGAC(2/6)
123 GTCTTC
BfaI 308 CTAG C^TAG
911 CTAG
BpmI 1040 CTCCAG CTGGAG(16/14)
332 CTGGAG
BpuAI 1510 GAAGAC GAAGAC(2/6)
123 GTCTTC
BsrI 1108 CCAGT ACTGG(1/-1)
1282 CCAGT
Cac8I 822 GCAAGC GC[ACGT]^[ACGT]GC
1169 GCCTGC
DsaI 31 CCGCGG C^C[GA][CT]GG
473 CCATGG
EaeI 1279 CGGCCA [CT]^GGCC[GA]
1356 TGGCCA
EcoT14I 473 CCATGG C^C[AT][AT]GG
596 CCAAGG
FauI 69 CCCGC CCCGC(4/6)
1161 CCCGC
HincII 419 GTCGAC GT[CT]^[GA]AC
535 GTCAAC
HindII 419 GTCGAC GT[CT]^[GA]AC
535 GTCAAC
HphI 41 TCACC GGTGA(8/7)
65 TCACC
MaeI 308 CTAG C^TAG
911 CTAG
MspA1I 31 CCGCGG C[AC]G^C[GT]G
956 CAGCTG
NlaIII 474 CATG CATG^
640 CATG
PleI 82 GAGTC GAGTC(4/5)
1363 GAGTC
PpuMI 52 GGGTCCC [GA]G^G[AT]CC[CT]
891 AGGTCCT
SacI 3 GAGCTC GAGCT^C
947 GAGCTC
SfaNI 1303 GATGC GCATC(5/9)
1422 GATGC
SfcI 479 CTACAG C^T[GA][CT]AG
1205 CTGCAG
StyI 473 CCATGG C^C[AT][AT]GG
596 CCAAGG
TfiI 699 GAATC G^A[AT]TC
719 GAATC
Tsp45I 40 GTCAC ^GT[GC]AC
115 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BmyI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIV Sau3AI Sau96I ScrFI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII