Restriction map for
pF1KB9297
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspLU11I BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I Fnu4HI FokI
FseI FspI Hin1I HincII HindII
HpaI ItaI KasI KpnI Ksp632I
KspI MfeI MluI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
TseI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 252 TCCGGA T^CCGGA
AflIII 620 ACACGT A^C[GA][CT]GT
AlwNI 304 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 321 GGGCCC GGGCC^C
AseI 792 ATTAAT AT^TAAT
AsnI 792 ATTAAT AT^TAAT
Asp700I 369 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 498 GAGCTC G[AT]GC[AT]^C
AvaII 360 GGACC G^G[AT]CC
BalI 89 TGGCCA TGG^CCA
BbsI 64 GTCTTC GAAGAC(2/6)
BclI 71 TGATCA T^GATCA
BcnI 324 CCCGG CC^[GC]GG
BfaI 881 CTAG C^TAG
BglII 650 AGATCT A^GATCT
BpuAI 64 GTCTTC GAAGAC(2/6)
BsaAI 621 CACGTA [CT]AC^GT[GA]
BsaWI 252 TCCGGA [AT]^CCGG[AT]
BseAI 252 TCCGGA T^CCGGA
BseRI 460 GAGGAG GAGGAG(10/8)
BsiHKAI 498 GAGCTC G[AT]GC[AT]^C
BsmAI 340 GTCTC GTCTC(1/5)
BspEI 252 TCCGGA T^CCGGA
BspHI 68 TCATGA T^CATGA
BspMI 534 GCAGGT ACCTGC(4/8)
BssSI 457 CACGAG CACGAG(-5/-1)
BstAPI 804 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 650 AGATCT [GA]^GATC[CT]
DraI 747 TTTAAA TTT^AAA
DraII 305 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 305 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 86 CCATGG C^C[AT][AT]GG
FbaI 71 TGATCA T^GATCA
HaeII 82 AGCGCC [GA]GCGC^[CT]
HgaI 891 GCGTC GACGC(5/10)
HindIII 757 AAGCTT A^AGCTT
HinfI 316 GACTC G^A[ACGT]TC
MaeI 881 CTAG C^TAG
MflI 650 AGATCT [GA]^GATC[CT]
MluNI 89 TGGCCA TGG^CCA
MroI 252 TCCGGA T^CCGGA
MscI 89 TGGCCA TGG^CCA
MspA1I 270 CAGCGG C[AC]G^C[GT]G
NciI 324 CCCGG CC^[GC]GG
NcoI 86 CCATGG C^CATGG
PleI 316 GACTC GAGTC(4/5)
PshBI 792 ATTAAT AT^TAAT
RcaI 68 TCATGA T^CATGA
SacI 498 GAGCTC GAGCT^C
SfcI 488 CTACAG C^T[GA][CT]AG
SmlI 223 CTCAAG C^T[CT][GA]AG
StyI 86 CCATGG C^C[AT][AT]GG
XhoII 650 AGATCT [GA]^GATC[CT]
XmnI 369 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 798 AAATTT [GA]^AATT[CT]
907 GAATTT
ApoI 798 AAATTT [GA]^AATT[CT]
907 GAATTT
BanII 321 GGGCCC G[GA]GC[CT]^C
498 GAGCTC
BmyI 321 GGGCCC G[AGT]GC[ACT]^C
498 GAGCTC
BpmI 19 CTGGAG CTGGAG(16/14)
310 CTGGAG
BsaBI 465 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
694 GATTACAATC
BsmFI 297 GTCCC GGGAC(10/14)
914 GTCCC
Bsp1286I 321 GGGCCC G[AGT]GC[ACT]^C
498 GAGCTC
Bsp1407I 35 TGTACA T^GTACA
594 TGTACA
BsrGI 35 TGTACA T^GTACA
594 TGTACA
BsrI 294 CCAGT ACTGG(1/-1)
818 CCAGT
CfoI 83 GCGC GCG^C
512 GCGC
DpnII 72 GATC ^GATC
651 GATC
DsaI 86 CCATGG C^C[GA][CT]GG
519 CCACGG
EaeI 89 TGGCCA [CT]^GGCC[GA]
566 CGGCCA
FauI 389 CCCGC CCCGC(4/6)
272 GCGGG
HapII 253 CCGG C^CGG
325 CCGG
HhaI 83 GCGC GCG^C
512 GCGC
HinP1I 83 GCGC G^CGC
512 GCGC
HpaII 253 CCGG C^CGG
325 CCGG
MaeII 395 ACGT A^CGT
622 ACGT
MamI 465 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
694 GATTACAATC
MboI 72 GATC ^GATC
651 GATC
MspI 253 CCGG C^CGG
325 CCGG
NdeII 72 GATC ^GATC
651 GATC
PflMI 303 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
635 CCACGCTCTGG
Sau3AI 72 GATC ^GATC
651 GATC
SspBI 35 TGTACA T^GTACA
594 TGTACA
Van91I 303 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
635 CCACGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AfaI AluI AvaI BsaJI
BsiYI BslI BsoBI BssKI BstNI
Cac8I Cfr13I DdeI EcoRII HaeIII
HphI MaeIII MboII MnlI MseI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI Tru9I Tsp45I
Tsp509I TspRI