Restriction map for
pF1KB5507
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BciVI BfrI BglI BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoT22I FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 1110 GTAC GT^AC
AflIII 1454 ACGTGT A^C[GA][CT]GT
Alw44I 458 GTGCAC G^TGCAC
ApaLI 458 GTGCAC G^TGCAC
AvaII 1131 GGACC G^G[AT]CC
BamHI 846 GGATCC G^GATCC
BbsI 1315 GAAGAC GAAGAC(2/6)
BcgI 1148 CGAGCAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 831 TGATCA T^GATCA
BglII 906 AGATCT A^GATCT
BlpI 66 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 66 GCTCAGC GC^T[ACGT]AGC
BpuAI 1315 GAAGAC GAAGAC(2/6)
BsgI 1552 CTGCAC GTGCAG(16/14)
BsmI 780 GAATGC GAATGC(1/-1)
BsrBI 1490 GAGCGG CCGCTC(-3/-3)
BsrDI 1497 CATTGC GCAATG(2/0)
BsrFI 1280 ACCGGC [GA]^CCGG[CT]
BstAPI 1536 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 66 GCTCAGC GC^T[ACGT]AGC
CfoI 774 GCGC GCG^C
Cfr10I 1280 ACCGGC [GA]^CCGG[CT]
DrdI 341 GACGTTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 95 CGGCCG C^GGCCG
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoNI 1580 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1400 GAATTC G^AATTC
FauI 439 CCCGC CCCGC(4/6)
FbaI 831 TGATCA T^GATCA
HhaI 774 GCGC GCG^C
HinP1I 774 GCGC G^CGC
KspI 37 CCGCGG CCGC^GG
NcoI 1167 CCATGG C^CATGG
NspI 1676 GCATGC [GA]CATG^[CT]
PaeR7I 880 CTCGAG C^TCGAG
PpuMI 1130 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 808 CAGCTG CAG^CTG
RsaI 1110 GTAC GT^AC
SacII 37 CCGCGG CCGC^GG
SbfI 1320 CCTGCAGG CCTGCA^GG
SexAI 1271 ACCAGGT A^CC[AT]GGT
SmlI 880 CTCGAG C^T[CT][GA]AG
SphI 1676 GCATGC GCATG^C
Sse8387I 1320 CCTGCAGG CCTGCA^GG
StuI 593 AGGCCT AGG^CCT
XhoI 880 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 38 CGCG CG^CG
773 CGCG
AspHI 458 GTGCAC G[AT]GC[AT]^C
994 GAGCAC
BanII 4 GAGCCC G[GA]GC[CT]^C
64 GGGCTC
BseRI 1358 GAGGAG GAGGAG(10/8)
1605 GAGGAG
BsiEI 34 CGACCG CG[GA][CT]^CG
95 CGGCCG
BsiHKAI 458 GTGCAC G[AT]GC[AT]^C
994 GAGCAC
BspMI 1319 ACCTGC ACCTGC(4/8)
419 GCAGGT
BssSI 287 CTCGTG CACGAG(-5/-1)
757 CTCGTG
BstEII 379 GGTGACC G^GT[ACGT]ACC
767 GGTTACC
BstPI 379 GGTGACC G^GT[ACGT]ACC
767 GGTTACC
BstUI 38 CGCG CG^CG
773 CGCG
DsaI 37 CCGCGG C^C[GA][CT]GG
1167 CCATGG
EaeI 95 CGGCCG [CT]^GGCC[GA]
1493 CGGCCA
EcoO65I 379 GGTGACC G^GT[ACGT]ACC
767 GGTTACC
EcoRV 925 GATATC GAT^ATC
1054 GATATC
EcoT14I 299 CCTTGG C^C[AT][AT]GG
1167 CCATGG
HincII 423 GTCAAC GT[CT]^[GA]AC
696 GTTGAC
HindII 423 GTCAAC GT[CT]^[GA]AC
696 GTTGAC
MspA1I 37 CCGCGG C[AC]G^C[GT]G
808 CAGCTG
MvnI 38 CGCG CG^CG
773 CGCG
PstI 1321 CTGCAG CTGCA^G
1534 CTGCAG
SapI 1736 GAAGAGC GCTCTTC(1/4)
823 GCTCTTC
StyI 299 CCTTGG C^C[AT][AT]GG
1167 CCATGG
TfiI 1075 GAATC G^A[AT]TC
1139 GAATC
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaI BbvI BcnI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII