################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AccIII AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvrII BamHI BbrPI BbsI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiHKAI BsiWI Bsp1407I BspDI BspEI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacI SacII SapI SfiI SfuI SmaI SnaBI SpeI SspBI SspI StyI SwaI TfiI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 1380 GTCGAC GT^[AC][GT]AC AclI 937 AACGTT AA^CGTT Asp700I 1438 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 715 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 810 TGCGCA TGC^GCA BalI 695 TGGCCA TGG^CCA BanI 981 GGCGCC G^G[CT][GA]CC BbeI 981 GGCGCC GGCGC^C BcgI 957 CGAGCAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1051 GGATAC GTATCC(6/5) BfaI 791 CTAG C^TAG BglI 211 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 800 AGATCT A^GATCT BsaWI 1067 ACCGGA [AT]^CCGG[AT] BsiEI 5 CGGCCG CG[GA][CT]^CG BsmBI 1367 GAGACG CGTCTC(1/5) BsmI 813 GCATTC GAATGC(1/-1) BspHI 1358 TCATGA T^CATGA BsrBI 102 CCGCTC CCGCTC(-3/-3) BssHII 173 GCGCGC G^CGCGC BstXI 992 CCAATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG FspI 810 TGCGCA TGC^GCA HaeII 981 GGCGCC [GA]GCGC^[CT] HgaI 1113 GACGC GACGC(5/10) HincII 1380 GTCGAC GT[CT]^[GA]AC HindII 1380 GTCGAC GT[CT]^[GA]AC HinfI 576 GACTC G^A[ACGT]TC KasI 981 GGCGCC G^GCGCC MaeI 791 CTAG C^TAG MluNI 695 TGGCCA TGG^CCA MscI 695 TGGCCA TGG^CCA NarI 981 GGCGCC GG^CGCC PflFI 715 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 576 GACTC GAGTC(4/5) PpuMI 612 GGGACCC [GA]G^G[AT]CC[CT] PshAI 576 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 937 AACGTT AA^CGTT RcaI 1358 TCATGA T^CATGA SalI 1380 GTCGAC G^TCGAC ScaI 1088 AGTACT AGT^ACT SexAI 716 ACCTGGT A^CC[AT]GGT SfoI 981 GGCGCC GGC^GCC SgrAI 210 CGCCGGCG C[GA]^CCGG[CT]G SphI 110 GCATGC GCATG^C StuI 440 AGGCCT AGG^CCT TspRI 1279 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 715 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1175 CCATCTACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1438 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 1132 GACGTC GACGT^C 1369 GACGTC AcsI 972 GAATTT [GA]^AATT[CT] 1034 AAATTT AlwI 24 GATCC GGATC(4/5) 785 GGATC ApoI 972 GAATTT [GA]^AATT[CT] 1034 AAATTT AvaI 595 CTCGGG C^[CT]CG[GA]G 955 CCCGAG AvaII 36 GGACC G^G[AT]CC 613 GGACC BbvCI 168 CCTCAGC CCTCAGC(-5/-2) 552 GCTGAGG BsoBI 595 CTCGGG C^[CT]CG[GA]G 955 CCCGAG BspLU11I 1106 ACATGT A^CATGT 1126 ACATGT BsrFI 136 GCCGGC [GA]^CCGG[CT] 211 GCCGGC BstAPI 153 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 354 GCAGCTGCTGC BstYI 23 AGATCC [GA]^GATC[CT] 800 AGATCT Cfr10I 136 GCCGGC [GA]^CCGG[CT] 211 GCCGGC DraIII 472 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1396 CACCGAGTG EaeI 5 CGGCCG [CT]^GGCC[GA] 695 TGGCCA MflI 23 AGATCC [GA]^GATC[CT] 800 AGATCT MseI 896 TTAA T^TAA 1319 TTAA NaeI 136 GCCGGC GCC^GGC 211 GCCGGC NgoMIV 136 GCCGGC G^CCGGC 211 GCCGGC PvuII 157 CAGCTG CAG^CTG 355 CAGCTG SbfI 333 CCTGCAGG CCTGCA^GG 432 CCTGCAGG SfaNI 964 GATGC GCATC(5/9) 1349 GCATC SmlI 1291 CTCAAG C^T[CT][GA]AG 1408 CTCAAG Sse8387I 333 CCTGCAGG CCTGCA^GG 432 CCTGCAGG TaqI 71 TCGA T^CGA 1381 TCGA Tru9I 896 TTAA T^TAA 1319 TTAA TthHB8I 71 TCGA T^CGA 1381 TCGA XhoII 23 AGATCC [GA]^GATC[CT] 800 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwNI ApaI BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PstI RsaI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I Tsp509I Van91I