Restriction map for
pF1KB6232
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsgI
BsiWI BsmBI BsmI BspHI BspLU11I
BspMI BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MfeI MflI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SacII SalI SapI
SbfI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tth111I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AflIII 630 ACGTGT A^C[GA][CT]GT
Aor51HI 933 AGCGCT AGC^GCT
BalI 1958 TGGCCA TGG^CCA
BsaAI 535 TACGTG [CT]AC^GT[GA]
BsaBI 445 GATATCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 917 GAGACC GGTCTC(1/5)
BsiEI 450 CGATCG CG[GA][CT]^CG
Bsp1407I 938 TGTACA T^GTACA
BspDI 448 ATCGAT AT^CGAT
BsrDI 1827 CATTGC GCAATG(2/0)
BsrGI 938 TGTACA T^GTACA
ClaI 448 ATCGAT AT^CGAT
DrdI 1725 GACCACGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 933 AGCGCT AGC^GCT
EcoRV 445 GATATC GAT^ATC
MamI 445 GATATCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1958 TGGCCA TGG^CCA
MscI 1958 TGGCCA TGG^CCA
MspA1I 1489 CAGCTG C[AC]G^C[GT]G
NaeI 992 GCCGGC GCC^GGC
NgoMIV 992 GCCGGC G^CCGGC
NspI 1142 GCATGC [GA]CATG^[CT]
PflMI 1544 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 868 CTGCAG CTGCA^G
PvuI 450 CGATCG CGAT^CG
PvuII 1489 CAGCTG CAG^CTG
ScaI 1094 AGTACT AGT^ACT
SexAI 693 ACCAGGT A^CC[AT]GGT
SfiI 1167 GGCCTTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1142 GCATGC GCATG^C
SspBI 938 TGTACA T^GTACA
Tsp509I 1249 AATT ^AATT
Van91I 1544 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 17 TCCGGA T^CCGGA
374 TCCGGA
Alw44I 645 GTGCAC G^TGCAC
1649 GTGCAC
AlwNI 257 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
1918 CAGGGCCTG
ApaLI 645 GTGCAC G^TGCAC
1649 GTGCAC
BbeI 64 GGCGCC GGCGC^C
1986 GGCGCC
BbvCI 24 GCTGAGG CCTCAGC(-5/-2)
2005 GCTGAGG
BfaI 439 CTAG C^TAG
460 CTAG
BseAI 17 TCCGGA T^CCGGA
374 TCCGGA
BseRI 760 GAGGAG GAGGAG(10/8)
1558 GAGGAG
BspEI 17 TCCGGA T^CCGGA
374 TCCGGA
BsrFI 992 GCCGGC [GA]^CCGG[CT]
1625 ACCGGC
BssSI 1570 CACGAG CACGAG(-5/-1)
1993 CACGAG
Cfr10I 992 GCCGGC [GA]^CCGG[CT]
1625 ACCGGC
EarI 1204 CTCTTC CTCTTC(1/4)
1834 CTCTTC
EcoNI 1203 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1781 CCTACGTCAGG
HincII 172 GTCAAC GT[CT]^[GA]AC
856 GTTGAC
HindII 172 GTCAAC GT[CT]^[GA]AC
856 GTTGAC
KasI 64 GGCGCC G^GCGCC
1986 GGCGCC
Ksp632I 1204 CTCTTC CTCTTC(1/4)
1834 CTCTTC
MaeI 439 CTAG C^TAG
460 CTAG
MroI 17 TCCGGA T^CCGGA
374 TCCGGA
MslI 358 CATGCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1666 CACCGCCATG
NarI 64 GGCGCC GG^CGCC
1986 GGCGCC
NcoI 1815 CCATGG C^CATGG
1865 CCATGG
PpuMI 1130 AGGACCC [GA]G^G[AT]CC[CT]
1598 GGGACCC
SacI 513 GAGCTC GAGCT^C
1320 GAGCTC
SfcI 684 CTACAG C^T[GA][CT]AG
868 CTGCAG
SfoI 64 GGCGCC GGC^GCC
1986 GGCGCC
SmaI 142 CCCGGG CCC^GGG
590 CCCGGG
StuI 729 AGGCCT AGG^CCT
1337 AGGCCT
TfiI 555 GAATC G^A[AT]TC
718 GAATC
XcmI 704 CCATGCGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1544 CCATGAGCTGGCTGG
XmaCI 142 CCCGGG C^CCGGG
590 CCCGGG
XmaI 142 CCCGGG C^CCGGG
590 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PaeR7I PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoI