Restriction map for
pF1KE0699
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# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI BsiEI BsiWI BsmBI BsrBI
BssHII Bst1107I BstZ17I CpoI EagI
Eam1105I EclXI Eco47III Eco52I FseI
KpnI KspI MluI NheI NotI
NruI PacI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SbfI
SfiI SgrAI SmaI SpeI Sse8387I
XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 9865 GACGTC GACGT^C
AviII 8717 TGCGCA TGC^GCA
AvrII 9855 CCTAGG C^CTAGG
BbeI 70 GGCGCC GGCGC^C
BbrPI 3673 CACGTG CAC^GTG
BcgI 630 GCAGTGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1579 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 9855 CCTAGG C^CTAGG
BspDI 5719 ATCGAT AT^CGAT
BspLU11I 7262 ACATGT A^CATGT
BsrFI 4535 GCCGGC [GA]^CCGG[CT]
Cfr10I 4535 GCCGGC [GA]^CCGG[CT]
ClaI 5719 ATCGAT AT^CGAT
DraIII 11849 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 12675 GACGGTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 8717 TGCGCA TGC^GCA
KasI 70 GGCGCC G^GCGCC
NaeI 4535 GCCGGC GCC^GGC
NarI 70 GGCGCC GG^CGCC
NgoMIV 4535 GCCGGC G^CCGGC
PaeR7I 3995 CTCGAG C^TCGAG
PmaCI 3673 CACGTG CAC^GTG
PmlI 3673 CACGTG CAC^GTG
SalI 5161 GTCGAC G^TCGAC
SapI 7816 GCTCTTC GCTCTTC(1/4)
SexAI 1238 ACCTGGT A^CC[AT]GGT
SfoI 70 GGCGCC GGC^GCC
SnaBI 12887 TACGTA TAC^GTA
SwaI 9071 ATTTAAAT ATTT^AAAT
XhoI 3995 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 18 CGCG CG^CG
1341 CGCG
AcyI 70 GGCGCC G[GA]^CG[CT]C
9865 GACGTC
AspI 2137 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3174 GACCTTGTC
BsaAI 3673 CACGTG [CT]AC^GT[GA]
12887 TACGTA
BsaHI 70 GGCGCC G[GA]^CG[CT]C
9865 GACGTC
Bsp1407I 4097 TGTACA T^GTACA
11126 TGTACA
BspHI 5829 TCATGA T^CATGA
13640 TCATGA
BsrGI 4097 TGTACA T^GTACA
11126 TGTACA
BstBI 4296 TTCGAA TT^CGAA
12696 TTCGAA
BstEII 1230 GGTCACC G^GT[ACGT]ACC
5473 GGTAACC
BstPI 1230 GGTCACC G^GT[ACGT]ACC
5473 GGTAACC
BstUI 18 CGCG CG^CG
1341 CGCG
EcoO65I 1230 GGTCACC G^GT[ACGT]ACC
5473 GGTAACC
EcoT22I 4367 ATGCAT ATGCA^T
9641 ATGCAT
HgaI 748 GACGC GACGC(5/10)
9405 GCGTC
Hin1I 70 GGCGCC G[GA]^CG[CT]C
9865 GACGTC
MvnI 18 CGCG CG^CG
1341 CGCG
NsiI 4367 ATGCAT ATGCA^T
9641 ATGCAT
NspV 4296 TTCGAA TT^CGAA
12696 TTCGAA
PflFI 2137 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3174 GACCTTGTC
RcaI 5829 TCATGA T^CATGA
13640 TCATGA
ScaI 6730 AGTACT AGT^ACT
10234 AGTACT
SfuI 4296 TTCGAA TT^CGAA
12696 TTCGAA
SspBI 4097 TGTACA T^GTACA
11126 TGTACA
StuI 1068 AGGCCT AGG^CCT
3016 AGGCCT
Tth111I 2137 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3174 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
AocI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BciVI BclI BcnI BfaI BfrI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI