Restriction map for
pF1KB8470
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# NO CUT ENZYMES ( 88 enzymes) #
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AccIII AclI AhdI ApaI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbeI BcgI BciVI BclI
BglI BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BseRI BsiWI BsmAI
BsmBI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KasI Ksp632I MamI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 2261 GGTACC G^GTACC
AgeI 1276 ACCGGT A^CCGGT
Alw44I 2017 GTGCAC G^TGCAC
AocI 1884 CCTCAGG CC^T[ACGT]AGG
Aor51HI 939 AGCGCT AGC^GCT
ApaLI 2017 GTGCAC G^TGCAC
Asp700I 396 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2261 GGTACC G^GTACC
AspI 1944 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2093 CCCGGG C^[CT]CG[GA]G
BanI 2261 GGTACC G^G[CT][GA]CC
BbrPI 2328 CACGTG CAC^GTG
BbsI 1713 GAAGAC GAAGAC(2/6)
BbvCI 1918 GCTGAGG CCTCAGC(-5/-2)
BpuAI 1713 GAAGAC GAAGAC(2/6)
BsaAI 2328 CACGTG [CT]AC^GT[GA]
BsgI 132 GTGCAG GTGCAG(16/14)
BsiEI 1278 CGGTCG CG[GA][CT]^CG
BsoBI 2093 CCCGGG C^[CT]CG[GA]G
BspLU11I 1002 ACATGT A^CATGT
BsrDI 1415 GCAATG GCAATG(2/0)
BsrFI 1276 ACCGGT [GA]^CCGG[CT]
BstEII 697 GGTAACC G^GT[ACGT]ACC
BstPI 697 GGTAACC G^GT[ACGT]ACC
BstXI 1542 CCAGTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1884 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1276 ACCGGT [GA]^CCGG[CT]
DraII 1868 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2011 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 381 GACGAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 939 AGCGCT AGC^GCT
Eco81I 1884 CCTCAGG CC^T[ACGT]AGG
EcoNI 8 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1868 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 697 GGTAACC G^GT[ACGT]ACC
HaeII 939 AGCGCT [GA]GCGC^[CT]
HincII 835 GTCAAC GT[CT]^[GA]AC
HindII 835 GTCAAC GT[CT]^[GA]AC
HindIII 263 AAGCTT A^AGCTT
KpnI 2261 GGTACC GGTAC^C
KspI 1982 CCGCGG CCGC^GG
NcoI 32 CCATGG C^CATGG
NdeI 715 CATATG CA^TATG
PflFI 1944 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2147 CCATTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1276 ACCGGT A^CCGGT
PmaCI 2328 CACGTG CAC^GTG
PmlI 2328 CACGTG CAC^GTG
PpuMI 1868 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1768 GAGCTC GAGCT^C
SacII 1982 CCGCGG CCGC^GG
ScaI 1544 AGTACT AGT^ACT
SexAI 701 ACCTGGT A^CC[AT]GGT
SmaI 2093 CCCGGG CCC^GGG
TaqI 875 TCGA T^CGA
Tth111I 1944 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 875 TCGA T^CGA
Van91I 2147 CCATTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2093 CCCGGG C^CCGGG
XmaI 2093 CCCGGG C^CCGGG
XmnI 396 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 1261 GACGTC GACGT^C
2203 GACGTC
AccI 1993 GTCTAC GT^[AC][GT]AC
2446 GTCTAC
AflII 747 CTTAAG C^TTAAG
1360 CTTAAG
AflIII 1002 ACATGT A^C[GA][CT]GT
2099 ACGTGT
AvaII 25 GGACC G^G[AT]CC
1869 GGACC
BcnI 2093 CCCGG CC^[GC]GG
2094 CCGGG
BfrI 747 CTTAAG C^TTAAG
1360 CTTAAG
BglII 788 AGATCT A^GATCT
1697 AGATCT
BsmI 1666 GAATGC GAATGC(1/-1)
905 GCATTC
Bsp1407I 2102 TGTACA T^GTACA
2177 TGTACA
BsrGI 2102 TGTACA T^GTACA
2177 TGTACA
BstAPI 1953 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2010 GCACTTCGTGC
CfoI 37 GCGC GCG^C
940 GCGC
EcoT14I 32 CCATGG C^C[AT][AT]GG
2432 CCAAGG
HhaI 37 GCGC GCG^C
940 GCGC
HinP1I 37 GCGC G^CGC
940 GCGC
MfeI 290 CAATTG C^AATTG
576 CAATTG
MunI 290 CAATTG C^AATTG
576 CAATTG
NciI 2093 CCCGG CC^[GC]GG
2094 CCGGG
NspI 451 ACATGC [GA]CATG^[CT]
1002 ACATGT
PvuII 1039 CAGCTG CAG^CTG
2266 CAGCTG
SspBI 2102 TGTACA T^GTACA
2177 TGTACA
SspI 547 AATATT AAT^ATT
966 AATATT
StyI 32 CCATGG C^C[AT][AT]GG
2432 CCAAGG
XcmI 937 CCAGCGCTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2036 CCAGGAACTGCCTGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
BalI BanII BbvI BfaI BmyI
BpmI BsaHI BsaJI BsaWI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII