Restriction map for
pF1KE2343
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BpuAI BsaBI BsaI BsiHKAI
BsmI BspDI BspHI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI DraI DraIII DrdI
Eam1105I EarI Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI Ksp632I
MamI MboII MfeI MseI MslI
MunI NcoI NdeI NheI NlaIII
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI SalI SapI SbfI
SexAI SfaNI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 58 GACGTC GACGT^C
Acc65I 494 GGTACC G^GTACC
AccI 1009 GTCTAC GT^[AC][GT]AC
AccIII 1951 TCCGGA T^CCGGA
AflIII 842 ACGCGT A^C[GA][CT]GT
Asp718I 494 GGTACC G^GTACC
AvrII 1894 CCTAGG C^CTAGG
BamHI 282 GGATCC G^GATCC
BbvCI 593 CCTCAGC CCTCAGC(-5/-2)
BlnI 1894 CCTAGG C^CTAGG
BsaAI 1660 TACGTG [CT]AC^GT[GA]
BsaWI 1951 TCCGGA [AT]^CCGG[AT]
BseAI 1951 TCCGGA T^CCGGA
BsiWI 845 CGTACG C^GTACG
BsmBI 60 CGTCTC CGTCTC(1/5)
Bsp1407I 605 TGTACA T^GTACA
BspEI 1951 TCCGGA T^CCGGA
BspMI 1530 GCAGGT ACCTGC(4/8)
BsrDI 437 GCAATG GCAATG(2/0)
BsrGI 605 TGTACA T^GTACA
BssSI 1064 CTCGTG CACGAG(-5/-1)
BstF5I 1176 GGATG GGATG(2/0)
CpoI 1276 CGGACCG CG^G[AT]CCG
EcoNI 1413 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 1176 GGATG GGATG(9/13)
HphI 1131 GGTGA GGTGA(8/7)
KpnI 494 GGTACC GGTAC^C
MluI 842 ACGCGT A^CGCGT
MroI 1951 TCCGGA T^CCGGA
NotI 1432 GCGGCCGC GC^GGCCGC
PflMI 87 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 1276 CGGACCG CG^G[AT]CCG
ScaI 197 AGTACT AGT^ACT
SgrAI 1636 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 605 TGTACA T^GTACA
StuI 339 AGGCCT AGG^CCT
Tsp45I 1132 GTGAC ^GT[GC]AC
Van91I 87 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 857 CCAAGGCCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 49 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1693 CAGATCCTG
Aor51HI 182 AGCGCT AGC^GCT
1314 AGCGCT
BfaI 1477 CTAG C^TAG
1895 CTAG
BsgI 1165 GTGCAG GTGCAG(16/14)
1759 GTGCAG
BsmAI 1093 GAGAC GTCTC(1/5)
61 GTCTC
BstAPI 437 GCAATGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1692 GCAGATCCTGC
BstYI 282 GGATCC [GA]^GATC[CT]
1694 AGATCC
DpnII 283 GATC ^GATC
1695 GATC
Eco47III 182 AGCGCT AGC^GCT
1314 AGCGCT
MaeI 1477 CTAG C^TAG
1895 CTAG
MaeII 59 ACGT A^CGT
1661 ACGT
MaeIII 509 GTTAC ^GT[ACGT]AC
1132 GTGAC
MboI 283 GATC ^GATC
1695 GATC
MflI 282 GGATCC [GA]^GATC[CT]
1694 AGATCC
NdeII 283 GATC ^GATC
1695 GATC
PleI 1095 GACTC GAGTC(4/5)
1918 GACTC
PpuMI 992 AGGTCCT [GA]G^G[AT]CC[CT]
1862 AGGACCT
PvuII 1342 CAGCTG CAG^CTG
1798 CAGCTG
Sau3AI 283 GATC ^GATC
1695 GATC
SmlI 988 CTCAAG C^T[CT][GA]AG
1198 CTCAAG
TfiI 214 GATTC G^A[AT]TC
778 GAATC
XhoII 282 GGATCC [GA]^GATC[CT]
1694 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssHII BssKI BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI KspI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIV PstI RsaI SacII Sau96I
ScrFI SfcI SfoI SmaI StyI
TseI TspRI XmaCI XmaI