Restriction map for
pF1KE2432
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvaI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI ScrFI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AlwNI 282 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 666 GGTCC G^G[AT]CC
BbsI 778 GTCTTC GAAGAC(2/6)
BbvCI 303 CCTCAGC CCTCAGC(-5/-2)
BbvI 627 GCAGC GCAGC(8/12)
BglII 119 AGATCT A^GATCT
BmyI 556 GTGCCC G[AGT]GC[ACT]^C
BpmI 159 CTCCAG CTGGAG(16/14)
BpuAI 778 GTCTTC GAAGAC(2/6)
BsaI 156 GGTCTC GGTCTC(1/5)
BsgI 509 GTGCAG GTGCAG(16/14)
Bsp1286I 556 GTGCCC G[AGT]GC[ACT]^C
BsrBI 100 GAGCGG CCGCTC(-3/-3)
BsrFI 271 ACCGGC [GA]^CCGG[CT]
BstF5I 831 CATCC GGATG(2/0)
Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC
Cfr10I 271 ACCGGC [GA]^CCGG[CT]
DraII 402 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 633 CCATGG C^C[GA][CT]GG
EaeI 273 CGGCCA [CT]^GGCC[GA]
EcoO109I 402 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 738 GATATC GAT^ATC
FauI 102 GCGGG CCCGC(4/6)
FokI 831 CATCC GGATG(9/13)
HapII 272 CCGG C^CGG
HincII 425 GTCAAC GT[CT]^[GA]AC
HindII 425 GTCAAC GT[CT]^[GA]AC
HindIII 677 AAGCTT A^AGCTT
HpaII 272 CCGG C^CGG
MslI 384 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 272 CCGG C^CGG
NcoI 633 CCATGG C^CATGG
NlaIV 402 GGGGCC GG[ACGT]^[ACGT]CC
PleI 759 GAGTC GAGTC(4/5)
TseI 627 GCAGC G^C[AT]GC
XcmI 29 CCAGTGTGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 60 AAATTT [GA]^AATT[CT]
90 AAATTC
AlwI 652 GATCC GGATC(4/5)
643 GGATC
ApoI 60 AAATTT [GA]^AATT[CT]
90 AAATTC
BfaI 299 CTAG C^TAG
544 CTAG
BsaJI 633 CCATGG C^C[ACGT][ACGT]GG
662 CCAAGG
BseRI 366 GAGGAG GAGGAG(10/8)
504 GAGGAG
BsiYI 95 CCATTGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCTGATTTGGG
BslI 95 CCATTGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCTGATTTGGG
BsmAI 111 GAGAC GTCTC(1/5)
157 GTCTC
Cfr13I 403 GGGCC G^G[ACGT]CC
666 GGTCC
DdeI 304 CTCAG C^T[ACGT]AG
552 CTTAG
EcoT14I 633 CCATGG C^C[AT][AT]GG
662 CCAAGG
Fnu4HI 627 GCAGC GC^[ACGT]GC
882 GCGGC
HaeIII 274 GGCC GG^CC
404 GGCC
HphI 390 GGTGA GGTGA(8/7)
900 GGTGA
ItaI 627 GCAGC GC^[ACGT]GC
882 GCGGC
MaeI 299 CTAG C^TAG
544 CTAG
MaeIII 268 GTAAC ^GT[ACGT]AC
535 GTAAC
MwoI 275 GCCATTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
396 GCTGTTGGGGC
Sau96I 403 GGGCC G^G[ACGT]CC
666 GGTCC
StyI 633 CCATGG C^C[AT][AT]GG
662 CCAAGG
TfiI 138 GATTC G^A[AT]TC
803 GATTC
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AfaI AluI BsrI BstYI
DpnII HinfI MboI MboII MflI
MnlI MseI NdeII NlaIII RsaI
Sau3AI SfaNI SmlI Tru9I Tsp509I
TspRI XhoII