Restriction map for
pF1KE2530
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# NO CUT ENZYMES ( 33 enzymes) #
##################################
AflII Aor51HI AscI AviII BbeI
BfrI BsiWI CpoI Eco47III EcoRV
FseI FspI HaeII KasI KspI
MluI NarI NheI NruI PacI
PmeI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SwaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 2187 GTATAC GT^[AC][GT]AC
AclI 2824 AACGTT AA^CGTT
AgeI 8839 ACCGGT A^CCGGT
AocI 6994 CCTCAGG CC^T[ACGT]AGG
ApaI 4880 GGGCCC GGGCC^C
AseI 1135 ATTAAT AT^TAAT
AsnI 1135 ATTAAT AT^TAAT
BbrPI 4638 CACGTG CAC^GTG
BciVI 4360 GGATAC GTATCC(6/5)
BlpI 3568 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3568 GCTGAGC GC^T[ACGT]AGC
BsiEI 70 CGGCCG CG[GA][CT]^CG
Bsp1407I 3268 TGTACA T^GTACA
BspDI 4299 ATCGAT AT^CGAT
BsrBI 710 CCGCTC CCGCTC(-3/-3)
BsrGI 3268 TGTACA T^GTACA
BssHII 1754 GCGCGC G^CGCGC
Bst1107I 2187 GTATAC GTA^TAC
BstBI 6464 TTCGAA TT^CGAA
BstEII 8666 GGTTACC G^GT[ACGT]ACC
BstPI 8666 GGTTACC G^GT[ACGT]ACC
BstZ17I 2187 GTATAC GTA^TAC
Bsu36I 6994 CCTCAGG CC^T[ACGT]AGG
CelII 3568 GCTGAGC GC^T[ACGT]AGC
ClaI 4299 ATCGAT AT^CGAT
DraI 5493 TTTAAA TTT^AAA
DrdI 799 GACCCCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 70 CGGCCG C^GGCCG
EclXI 70 CGGCCG C^GGCCG
Eco52I 70 CGGCCG C^GGCCG
Eco81I 6994 CCTCAGG CC^T[ACGT]AGG
EcoO65I 8666 GGTTACC G^GT[ACGT]ACC
NcoI 8078 CCATGG C^CATGG
NotI 69 GCGGCCGC GC^GGCCGC
NspV 6464 TTCGAA TT^CGAA
PinAI 8839 ACCGGT A^CCGGT
PmaCI 4638 CACGTG CAC^GTG
PmlI 4638 CACGTG CAC^GTG
PshBI 1135 ATTAAT AT^TAAT
Psp1406I 2824 AACGTT AA^CGTT
SapI 7123 GCTCTTC GCTCTTC(1/4)
ScaI 4511 AGTACT AGT^ACT
SfuI 6464 TTCGAA TT^CGAA
SspBI 3268 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 40 enzymes) #
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AatII 2302 GACGTC GACGT^C
6786 GACGTC
Acc65I 6686 GGTACC G^GTACC
7070 GGTACC
AccIII 40 TCCGGA T^CCGGA
3545 TCCGGA
AcyI 2302 GACGTC G[GA]^CG[CT]C
6786 GACGTC
AhdI 2638 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8238 GACACATGGTC
Asp718I 6686 GGTACC G^GTACC
7070 GGTACC
AspEI 2638 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8238 GACACATGGTC
AspI 4618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6220 GACAGCGTC
AvrII 2218 CCTAGG C^CTAGG
7720 CCTAGG
BalI 3888 TGGCCA TGG^CCA
8532 TGGCCA
BbvCI 1690 CCTCAGC CCTCAGC(-5/-2)
4873 CCTCAGC
BglI 47 GCCTCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3443 GCCGGCCTGGC
BglII 3678 AGATCT A^GATCT
5838 AGATCT
BlnI 2218 CCTAGG C^CTAGG
7720 CCTAGG
BsaHI 2302 GACGTC G[GA]^CG[CT]C
6786 GACGTC
BsaI 663 GAGACC GGTCTC(1/5)
7148 GAGACC
BseAI 40 TCCGGA T^CCGGA
3545 TCCGGA
BspEI 40 TCCGGA T^CCGGA
3545 TCCGGA
BspMI 1925 ACCTGC ACCTGC(4/8)
393 GCAGGT
BssSI 7145 CACGAG CACGAG(-5/-1)
4927 CTCGTG
Eam1105I 2638 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8238 GACACATGGTC
EcoT22I 3087 ATGCAT ATGCA^T
7043 ATGCAT
HgaI 6224 GCGTC GACGC(5/10)
8726 GCGTC
Hin1I 2302 GACGTC G[GA]^CG[CT]C
6786 GACGTC
HpaI 2107 GTTAAC GTT^AAC
2233 GTTAAC
KpnI 6686 GGTACC GGTAC^C
7070 GGTACC
MluNI 3888 TGGCCA TGG^CCA
8532 TGGCCA
MroI 40 TCCGGA T^CCGGA
3545 TCCGGA
MscI 3888 TGGCCA TGG^CCA
8532 TGGCCA
NaeI 1758 GCCGGC GCC^GGC
3443 GCCGGC
NgoMIV 1758 GCCGGC G^CCGGC
3443 GCCGGC
NsiI 3087 ATGCAT ATGCA^T
7043 ATGCAT
PaeR7I 3953 CTCGAG C^TCGAG
5114 CTCGAG
PflFI 4618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6220 GACAGCGTC
SmaI 5057 CCCGGG CCC^GGG
5282 CCCGGG
Tth111I 4618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6220 GACAGCGTC
XbaI 5196 TCTAGA T^CTAGA
6810 TCTAGA
XhoI 3953 CTCGAG C^TCGAG
5114 CTCGAG
XmaCI 5057 CCCGGG C^CCGGG
5282 CCCGGG
XmaI 5057 CCCGGG C^CCGGG
5282 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvI
BcgI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaBI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SphI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI