Restriction map for
pF1KE5841
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BbsI BcgI BfrI
BlnI BpuAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BsrGI
BssHII BstBI Bsu36I ClaI CpoI
DraI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FseI HincII HindII HpaI KpnI
Ksp632I KspI MfeI MluI MunI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SfuI SgrAI SnaBI SpeI
SspBI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccIII 4688 TCCGGA T^CCGGA
BbrPI 947 CACGTG CAC^GTG
BciVI 386 GTATCC GTATCC(6/5)
BglII 5375 AGATCT A^GATCT
BsaBI 976 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4688 TCCGGA [AT]^CCGG[AT]
BseAI 4688 TCCGGA T^CCGGA
BspEI 4688 TCCGGA T^CCGGA
BspLU11I 1326 ACATGT A^CATGT
Bst1107I 1084 GTATAC GTA^TAC
BstZ17I 1084 GTATAC GTA^TAC
DrdI 3095 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3572 ATGCAT ATGCA^T
HindIII 2897 AAGCTT A^AGCTT
MamI 976 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4688 TCCGGA T^CCGGA
MseI 3920 TTAA T^TAA
NdeI 2756 CATATG CA^TATG
NsiI 3572 ATGCAT ATGCA^T
PaeR7I 3835 CTCGAG C^TCGAG
PmaCI 947 CACGTG CAC^GTG
PmlI 947 CACGTG CAC^GTG
PshAI 1255 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 243 CCTGCAGG CCTGCA^GG
ScaI 806 AGTACT AGT^ACT
SexAI 4919 ACCAGGT A^CC[AT]GGT
SmaI 1762 CCCGGG CCC^GGG
Sse8387I 243 CCTGCAGG CCTGCA^GG
SspI 2840 AATATT AAT^ATT
StuI 2288 AGGCCT AGG^CCT
Tru9I 3920 TTAA T^TAA
XhoI 3835 CTCGAG C^TCGAG
XmaCI 1762 CCCGGG C^CCGGG
XmaI 1762 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 752 GTCTAC GT^[AC][GT]AC
1084 GTATAC
AccII 4304 CGCG CG^CG
5107 CGCG
Alw44I 875 GTGCAC G^TGCAC
916 GTGCAC
ApaLI 875 GTGCAC G^TGCAC
916 GTGCAC
Asp700I 258 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4557 GAAGGACTTC
AviII 2293 TGCGCA TGC^GCA
5339 TGCGCA
BclI 2691 TGATCA T^GATCA
5121 TGATCA
BsaI 3401 GAGACC GGTCTC(1/5)
1299 GGTCTC
BssSI 2503 CACGAG CACGAG(-5/-1)
3440 CTCGTG
BstEII 396 GGTCACC G^GT[ACGT]ACC
1755 GGTGACC
BstPI 396 GGTCACC G^GT[ACGT]ACC
1755 GGTGACC
BstUI 4304 CGCG CG^CG
5107 CGCG
EcoO65I 396 GGTCACC G^GT[ACGT]ACC
1755 GGTGACC
FbaI 2691 TGATCA T^GATCA
5121 TGATCA
FspI 2293 TGCGCA TGC^GCA
5339 TGCGCA
MvnI 4304 CGCG CG^CG
5107 CGCG
SfiI 205 GGCCTGAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1522 GGCCACTGGGGCC
XmnI 258 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4557 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SphI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII