Restriction map for
pF1KE3717
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# NO CUT ENZYMES ( 53 enzymes) #
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AccIII AclI AflIII AgeI Aor51HI
AscI AspI AviII AvrII BbrPI
BcgI BlnI BseAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BssHII
BssSI BstBI ClaI CpoI DraIII
DrdI Eco47III EcoRI FseI FspI
HindIII HpaI MluI MroI NruI
NspV PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfiI SfuI
Sse8387I SwaI Tth111I
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 1153 GGTACC G^GTACC
AhdI 6184 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3209 GTGCAC G^TGCAC
AocI 1894 CCTGAGG CC^T[ACGT]AGG
ApaLI 3209 GTGCAC G^TGCAC
AseI 1689 ATTAAT AT^TAAT
AsnI 1689 ATTAAT AT^TAAT
Asp700I 6410 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1153 GGTACC G^GTACC
AspEI 6184 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 7044 TGGCCA TGG^CCA
BamHI 1978 GGATCC G^GATCC
BglII 1289 AGATCT A^GATCT
BsaAI 1511 TACGTA [CT]AC^GT[GA]
BsmI 3722 GCATTC GAATGC(1/-1)
Bsp1407I 4836 TGTACA T^GTACA
BsrBI 2488 CCGCTC CCGCTC(-3/-3)
BsrGI 4836 TGTACA T^GTACA
Bst1107I 6303 GTATAC GTA^TAC
BstAPI 5596 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 6448 GGTAACC G^GT[ACGT]ACC
BstPI 6448 GGTAACC G^GT[ACGT]ACC
BstZ17I 6303 GTATAC GTA^TAC
Bsu36I 1894 CCTGAGG CC^T[ACGT]AGG
Eam1105I 6184 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1894 CCTGAGG CC^T[ACGT]AGG
EcoO65I 6448 GGTAACC G^GT[ACGT]ACC
EcoT22I 1374 ATGCAT ATGCA^T
KpnI 1153 GGTACC GGTAC^C
MluNI 7044 TGGCCA TGG^CCA
MscI 7044 TGGCCA TGG^CCA
NdeI 2027 CATATG CA^TATG
NheI 1825 GCTAGC G^CTAGC
NsiI 1374 ATGCAT ATGCA^T
PacI 1690 TTAATTAA TTAAT^TAA
PaeR7I 2000 CTCGAG C^TCGAG
PshBI 1689 ATTAAT AT^TAAT
SapI 613 GAAGAGC GCTCTTC(1/4)
SgrAI 536 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2655 CCCGGG CCC^GGG
SnaBI 1511 TACGTA TAC^GTA
SspBI 4836 TGTACA T^GTACA
StuI 3914 AGGCCT AGG^CCT
XcmI 4163 CCAGCACAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2000 CTCGAG C^TCGAG
XmaCI 2655 CCCGGG C^CCGGG
XmaI 2655 CCCGGG C^CCGGG
XmnI 6410 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AatII 912 GACGTC GACGT^C
6714 GACGTC
AflII 4017 CTTAAG C^TTAAG
6553 CTTAAG
BbeI 10 GGCGCC GGCGC^C
164 GGCGCC
BbvCI 2215 CCTCAGC CCTCAGC(-5/-2)
2794 GCTGAGG
BciVI 4341 GTATCC GTATCC(6/5)
4796 GTATCC
BfrI 4017 CTTAAG C^TTAAG
6553 CTTAAG
BsiEI 125 CGGCCG CG[GA][CT]^CG
203 CGGCCG
BsrDI 4026 CATTGC GCAATG(2/0)
3245 GCAATG
DraI 793 TTTAAA TTT^AAA
946 TTTAAA
EagI 125 CGGCCG C^GGCCG
203 CGGCCG
EclXI 125 CGGCCG C^GGCCG
203 CGGCCG
Eco52I 125 CGGCCG C^GGCCG
203 CGGCCG
EcoNI 4657 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5338 CCTTTTCCAGG
EcoRV 607 GATATC GAT^ATC
6196 GATATC
HincII 1207 GTTGAC GT[CT]^[GA]AC
1963 GTTGAC
HindII 1207 GTTGAC GT[CT]^[GA]AC
1963 GTTGAC
KasI 10 GGCGCC G^GCGCC
164 GGCGCC
KspI 122 CCGCGG CCGC^GG
323 CCGCGG
MfeI 5395 CAATTG C^AATTG
6765 CAATTG
MunI 5395 CAATTG C^AATTG
6765 CAATTG
NaeI 60 GCCGGC GCC^GGC
537 GCCGGC
NarI 10 GGCGCC GG^CGCC
164 GGCGCC
NgoMIV 60 GCCGGC G^CCGGC
537 GCCGGC
NotI 124 GCGGCCGC GC^GGCCGC
202 GCGGCCGC
NspI 1232 GCATGC [GA]CATG^[CT]
5016 GCATGC
PflMI 174 CCACACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7053 CCAACCTGTGG
PshAI 1102 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1966 GACAGTGGTC
SacI 5009 GAGCTC GAGCT^C
6108 GAGCTC
SacII 122 CCGCGG CCGC^GG
323 CCGCGG
SfoI 10 GGCGCC GGC^GCC
164 GGCGCC
SpeI 5043 ACTAGT A^CTAGT
5542 ACTAGT
SphI 1232 GCATGC GCATG^C
5016 GCATGC
SspI 835 AATATT AAT^ATT
2875 AATATT
Van91I 174 CCACACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7053 CCAACCTGTGG
XbaI 4962 TCTAGA T^CTAGA
5376 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII